Purchase of a CD Spectropolarimeter

购买 CD 分光旋光计

• 批准号: 7792474
• 负责人: DEBORAH S. WUTTKE
• 金额: $ 19.45万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2011-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792474
• 关键词: Arts; Binding; Biology; Biomedical Research; Biophysics; Biotechnology; Circular Dichroism; Colorado; Equipment; Funding; Humpback Dolphins; Image; Membrane Proteins; Metals; Molecular; Neurons; Pathogenesis; Peptides; Pharmacologic Substance; Property; RNA; Research; Research Personnel; Resources; Science; Structure; Thermodynamics; Universities; Yin; base; design; in vivo; insight; instrument; instrumentation; programs; sensor; telomere; therapeutic protein; therapeutic target; tool

DESCRIPTION (provided by applicant): A group of researchers at the University of Colorado, Boulder, are requesting a state-of-the-art circular dichroism spectropolarimeter (CD) for biomolecular studies. CD is a key biophysical tool for the characterization of secondary and tertiary structure of biomolecules, and can provide insights into structure, as well as folding and binding thermodynamics. There is currently no functioning CD instrumentation on the Boulder campus, and this lack of resources has impeded progress in several NIH-funded research programs. These programs span a wide range of biomolecular science, including the study of therapeutic proteins and their aggregation properties (Carpenter, Randolph), membrane proteins and peptide therapeutics targeting neurons (Falke, Yin), bacterial pathogenesis (Sousa), telomere biology (Wuttke), the design of metal sensors for in vivo imaging (Palmer) and RNA bioscience (Batey and Pardi). Furthermore, the University of Colorado at Boulder has well-established programs in Molecular Biophysics and Pharmaceutical Biotechnology, and we anticipate the user base will grow once the instrumentation is established. A modern CD instrument is urgently needed at CU-Boulder, and acquisition of this equipment will have a broad impact across a wide range of biomedical research programs.

描述（由申请人提供）：位于博尔德的科罗拉多大学的一组研究人员正在申请一种用于生物分子研究的最先进的圆二色谱分光偏振仪（CD）。CD是表征生物分子二级和三级结构的关键生物物理工具，可以提供结构以及折叠和结合热力学的见解。目前在博尔德校区没有运行的CD仪器，这种资源的缺乏阻碍了NIH资助的几个研究项目的进展。这些项目涵盖了广泛的生物分子科学，包括治疗性蛋白质及其聚集特性的研究（Carpenter，兰多夫），靶向神经元的膜蛋白和肽治疗（Falke，Yin），细菌发病机制（Sousa），端粒生物学（Wuttke），体内成像金属传感器的设计（Palmer）和RNA生物科学（Batey和Pardi）。此外，位于博尔德的科罗拉多大学在分子生物物理学和药物生物技术方面有完善的计划，我们预计一旦仪器建立，用户群将增长。一个现代化的CD仪器是迫切需要在CU博尔德，并获得这种设备将在广泛的生物医学研究计划产生广泛的影响。

项目成果

Long-range energy transfer in self-assembled quantum dot-DNA cascades.

• DOI: 10.1039/c5nr04778a
• 发表时间: 2015-11
• 期刊: Nanoscale
• 影响因子: 6.7
• 作者: Samuel M Goodman;A. Siu;Vivek Singh;P. Nagpal

Reconstitution of human shelterin complexes reveals unexpected stoichiometry and dual pathways to enhance telomerase processivity.

• DOI: 10.1038/s41467-017-01313-w
• 发表时间: 2017-10-20
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Lim CJ;Zaug AJ;Kim HJ;Cech TR
• 通讯作者: Cech TR