A multiphoton microscope for translational and basic biomedical research

描述(由申请人提供)：这项提案寻求资金购买奥林巴斯FV1000MPE多光子显微镜，作为威尔康奈尔医学院核心设施的主要仪器。核心设施为翻译和基础医学研究人员提供服务。除了威尔·康奈尔大学的研究人员外，特殊外科医院的研究人员也将使用该设施。此外，该仪器将作为与伊萨卡康奈尔大学科学家合作的大型项目的主要仪器，这些科学家正在开发用于人类患者护理的新方法，包括多光子内窥镜检查。这个合作项目也是位于威尔康奈尔大学的临床和翻译科学中心项目的一个组成部分。该设备的最大用户将是一个由外科医生、病理学家、显微镜医生、工程师和物理学家组成的合作小组，他们正在检查未染色的新鲜人体活检样本，以创建通过自发荧光和二次谐波产生看到的正常和病变人体组织的图谱。初步数据表明，这种图像可以为某些类型的癌症提供可与传统组织病理学相媲美的诊断信息。来自胶原蛋白的SHG还将用于检查人体骨活检样本，以研究骨质疏松症。几项研究将在活体动物中进行成像，其中几个项目将受益于基于FV1000MPE的协同互动。例如，三名研究人员将检查大脑中的事件，其中两人合作研究阿尔茨海默病淀粉样斑块的形成和降解。两个项目将检查小鼠的巨噬细胞和树突状细胞，并将研究细胞迁移等特性。FV1000MPE非常适合这组调查人员的需求。它拥有一流的光学系统，最适合多光子显微镜和相对有效的组织深层成像。它很容易使用，这将允许对具有不同背景的调查人员进行快速培训。由于微探针物镜的直径很小，因此对动物成像非常有利，而且对于检查形状不规则的手术标本也非常有用，因为在送去进行病理处理之前，这些标本不能被切割或改变。

DESCRIPTION (provided by applicant): This proposal seeks funds to purchase an Olympus FV1000MPE multiphoton microscope to be used as the main instrument in a core facility at the Weill Cornell Medical College. The core facility serves translational and basic medical science investigators. In addition to investigators at Weill Cornell, researchers from the Hospital for Special Surgery will be users of the facility. Additionally, this instrument will serve as the major instrument for a large collaborative project with scientists at Cornell University in Ithaca who are developing novel methods, including multiphoton endoscopy, for use in human patient care. This collaborative project is also a component of the Clinical and Translational Science Center Project based at Weill Cornell. The largest user of the facility will be a collaborative group of surgeons, pathologists, microscopists, engineers, and physicists who are examining unstained fresh human biopsy samples to create an atlas of normal and diseased human tissue as seen by autofluorescence and second harmonic generation. Preliminary data suggest that such images can provide diagnostic information comparable to conventional histopathology for certain types of cancers. SHG from collagen will also be used for examining human bone biopsy specimens for studies of osteoporosis. Several studies will carry out imaging in living animals, and several of these projects will benefit from synergistic interactions based on the use of the FV1000MPE. For example, three investigators will examine events in the brain, and two of these collaborate on studies of the formation and degradation of Alzheimer's disease amyloid plaques. Two projects will examine macrophages and dendritic cells in mice and will investigate properties such as cell migration. The FV1000MPE is very well suited to serve the needs of this group of investigators. It has superb optics that are optimized for multiphoton microscopy and for relatively effective imaging deep into tissue. It is easy to use, which will allow rapid training of investigators with diverse backgrounds. The microprobe objectives are very advantageous for imaging in animals because of their small diameter, and these will also be very useful for examination of irregularly shaped surgical specimens, which cannot be cut or altered before being sent for pathology processing.