A multiphoton microscope for translational and basic biomedical research

用于转化和基础生物医学研究的多光子显微镜

• 批准号: 7842170
• 负责人: Frederick R. Maxfield
• 金额: $ 63.83万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2011-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7842170
• 关键词: Alzheimer' s Disease; Animals; Atlases; Biomedical Research; Biopsy Specimen; Brain; Caliber; Center for Translational Science Activities; Clinical; Collagen; Core Facility; Data; Dendritic Cells; Diagnostic; Endoscopy; Engineering; Event; Funding; Generations; Histopathology; Human; Image; Life; Medical; Methods; Microscope; Microscopy; Mus; Operative Surgical Procedures; Optics; Osteoporosis; Pathologist; Pathology processes; Patient Care; Property; Research Personnel; Science; Scientist; Senile Plaques; Shapes; Special Hospitals; Specimen; Surgeon; Tissues; Universities; base; bone; cancer type; cell motility; human tissue; instrument; investigator training; macrophage; medical schools; novel; second harmonic

DESCRIPTION (provided by applicant): This proposal seeks funds to purchase an Olympus FV1000MPE multiphoton microscope to be used as the main instrument in a core facility at the Weill Cornell Medical College. The core facility serves translational and basic medical science investigators. In addition to investigators at Weill Cornell, researchers from the Hospital for Special Surgery will be users of the facility. Additionally, this instrument will serve as the major instrument for a large collaborative project with scientists at Cornell University in Ithaca who are developing novel methods, including multiphoton endoscopy, for use in human patient care. This collaborative project is also a component of the Clinical and Translational Science Center Project based at Weill Cornell. The largest user of the facility will be a collaborative group of surgeons, pathologists, microscopists, engineers, and physicists who are examining unstained fresh human biopsy samples to create an atlas of normal and diseased human tissue as seen by autofluorescence and second harmonic generation. Preliminary data suggest that such images can provide diagnostic information comparable to conventional histopathology for certain types of cancers. SHG from collagen will also be used for examining human bone biopsy specimens for studies of osteoporosis. Several studies will carry out imaging in living animals, and several of these projects will benefit from synergistic interactions based on the use of the FV1000MPE. For example, three investigators will examine events in the brain, and two of these collaborate on studies of the formation and degradation of Alzheimer's disease amyloid plaques. Two projects will examine macrophages and dendritic cells in mice and will investigate properties such as cell migration. The FV1000MPE is very well suited to serve the needs of this group of investigators. It has superb optics that are optimized for multiphoton microscopy and for relatively effective imaging deep into tissue. It is easy to use, which will allow rapid training of investigators with diverse backgrounds. The microprobe objectives are very advantageous for imaging in animals because of their small diameter, and these will also be very useful for examination of irregularly shaped surgical specimens, which cannot be cut or altered before being sent for pathology processing.

描述（由申请人提供）：本提案寻求资金购买奥林巴斯FV1000MPE多光子显微镜，用作威尔康奈尔医学院核心设施的主要仪器。核心设施为翻译和基础医学科学研究人员提供服务。除了威尔康奈尔大学的研究人员外，特殊外科医院的研究人员也将成为该设施的用户。此外，该仪器将作为与伊萨卡康奈尔大学科学家合作的大型合作项目的主要仪器，该项目正在开发用于人类患者护理的新方法，包括多光子内窥镜检查。这个合作项目也是位于威尔康奈尔大学的临床和转化科学中心项目的一个组成部分。该设施的最大用户将是一个由外科医生、病理学家、显微镜学家、工程师和物理学家组成的合作小组，他们正在检查未染色的新鲜人体活检样本，以创建通过自体荧光和二次谐波产生所看到的正常和患病人体组织的图谱。初步数据表明，对于某些类型的癌症，这种图像可以提供与传统组织病理学相当的诊断信息。来自胶原的SHG也将用于检查人骨活检标本，用于骨质疏松症研究。几项研究将在活体动物中进行成像，其中几个项目将受益于基于FV1000MPE使用的协同作用。例如，三名研究人员将检查大脑中的事件，其中两人合作研究阿尔茨海默病淀粉样蛋白斑块的形成和降解。两个项目将检查小鼠的巨噬细胞和树突细胞，并将研究细胞迁移等特性。FV1000MPE非常适合满足这类研究者的需求。它具有卓越的光学性能，可用于多光子显微镜和组织深处的相对有效成像。它易于使用，这将使具有不同背景的调查人员能够迅速接受培训。微探针物镜由于其直径小，对于动物成像非常有利，并且这些对于检查不规则形状的手术标本也非常有用，这些标本在被送去进行病理处理之前不能被切割或改变。