Macrophage-Lipoprotein Interactions
Macrophage-Lipoprotein Interactions
批准号:
10584618
负责人:
Frederick R. Maxfield
金额:
$42.38万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
未结题
起止时间:
2009-07-01 至 2026-03-31
关键词:
3-DimensionalAGFG1 geneAcid LipaseActinsAnimal ModelArterial Fatty StreakAtherosclerosisBiochemistryBiological AssayBiological ProcessBlood VesselsBone MarrowCell Culture TechniquesCellsCellular biologyCholesterolCholesterol EstersConfocal MicroscopyDataDepositionDevelopmentElectron MicroscopyEnzymesEventExocytosisExtracellular MatrixFluorescence MicroscopyFoam CellsFunctional disorderGenerationsGoalsHumanHydrolaseHydrolysisImmune systemInflammasomeInflammatoryInflammatory ResponseIonsKnockout MiceLipidsLipoproteinsLow-Density LipoproteinsLysosomesMacrophageMacrophage ActivationMethodsMicroscopyModelingMolecularMorphologyMusOpticsPIK3CG genePhagocytesPolymersProcessProductionProtein Kinase CProteinsPublishingRNA InterferenceReportingResolutionRisk AssessmentRoleSNAP receptorScanning Electron MicroscopySignal PathwaySignal TransductionSignaling MoleculeSiteSmall Interfering RNAStructureSynapsesTREM2 geneTherapeutic InterventionTransplantationWorkcrosslinkextracellularimprovedin vivoknock-downmicroscopic imagingmouse modelnovelnovel therapeutic interventionnovel therapeuticsoptical imagingphospholipase C gammaresponsesealtherapy developmentthree dimensional structure
中文摘要
与细胞外基质紧密交联的脂蛋白聚集体是主要类型
动脉粥样硬化病变中的脂蛋白。这些聚集体中的大部分胆固醇是未酯化的,但它
一直不清楚细胞外滞留和聚集的低密度脂蛋白核心中的胆固醇酯是如何
水解性--特别是因为据报道涉及溶酶体水解酶。我们最近的研究
证明了保留和聚集的核心中胆固醇酯的一种新的水解机理
巨噬细胞(M)在聚集的部分周围创建紧密密封的隔室
低密度脂蛋白。然后,它们使这些隔室酸化,并将溶酶体酶分泌到其中,产生溶酶体
Synapse。已有研究表明,溶酶体酸性脂肪酶在胞外对胆固醇酯的降解,在
被称为消化外噬的过程,导致细胞外产生未酯化的胆固醇,以及
这种胆固醇进入细胞的运输导致泡沫细胞的形成。人体内高浓度的胆固醇
在初步研究中观察到聚集的低密度脂蛋白导致细胞外胆固醇的形成
晶体,可引起M的炎症反应。这项提议的首要假设是
这种降解脂蛋白的机制与传统的吞噬机制有显著的不同
或内吞机制,这些差异在脑血管疾病的病理生理学中有重要的影响。
动脉硬化。此外,了解这一过程可能会导致改善治疗干预。
第一个目标的工作将描述消化外噬的分子机制。这将是
包括溶酶体胞吐所需的Rab和SNARE蛋白的研究。通过Tlr4发送信号,
MyD88、PI3-激酶、Akt、Syk、Vav、CDC42等分子在消化过程中起重要作用
外噬作用,以及其他信号分子的作用将被探索。
在聚集的低密度脂蛋白中产生高浓度的胆固醇,在目标2中形成
将检查胆固醇晶体和由此引起的M-的炎症激活。
第三个目标的工作将使用光学成像和三维电子显微镜(FIB-SEM)来检查
小鼠动脉粥样硬化模型中溶酶体突触的三维结构。形成溶酶体突触,
胆固醇晶体与滞留和聚集的低密度脂蛋白以及炎症激活的关系将是
用光学显微镜对不同的小鼠动脉粥样硬化模型进行了研究。
更好地了解动脉粥样硬化病变中发生的细胞和分子事件可以导致
更好的风险评估和潜在的新疗法。
英文摘要
Aggregates of lipoproteins that are tightly crosslinked to the extracellular matrix are the major type of
lipoprotein in atherosclerotic lesions. The majority of the cholesterol in these aggregates is unesterified, but it
has been unclear how the cholesteryl esters in the core of retained and aggregated extracellular LDL are
hydrolyzed – especially because a lysosomal hydrolase has been reported to be involved. Our recent studies
demonstrate a novel mechanism for the hydrolysis of cholesteryl esters in the core of retained and aggregated
LDL in which macrophages (M) create tightly sealed compartments that surround portions of the aggregated
LDL. They then acidify these compartments and secrete lysosomal enzymes into them, creating a lysosomal
synapse. It has been shown that the extracellular hydrolysis of cholesteryl esters by lysosomal acid lipase, in a
process called digestive exophagy, leads to production of unesterified cholesterol outside the cell, and
transport of this cholesterol into the cell leads to foam cell formation. The high concentrations of cholesterol in
the aggregated LDL were observed in a preliminary study to lead to the formation of extracellular cholesterol
crystals, which can cause inflammatory responses in M . The overarching hypothesis of this proposal is that
this mechanism for degrading lipoproteins has significant differences as compared to conventional phagocytic
or endocytic mechanisms and that these differences have important consequences in the pathophysiology of
atherosclerosis. Furthermore, understanding this process may lead to improved therapeutic interventions.
Work in the first aim will characterize the molecular mechanisms of digestive exophagy. This will
include a study of the Rab and SNARE proteins that are required for lysosomal exocytosis. Signaling by Tlr4,
Myd88, PI3-kinase, Akt, Syk, Vav, Cdc42, and other molecules has been shown to be important for digestive
exophagy, and the roles of additional signaling molecules will be explored.
High concentrations of cholesterol are generated in aggregated LDL, and in Aim 2 formation of
cholesterol crystals and resulting inflammatory activation of M will be examined.
Work in the third aim will use optical imaging and 3-D electron microscopy (FIB-SEM) to examine the
3D structures of lysosomal synapses in a mouse atherosclerosis model. Formation of lysosomal synapses,
association of cholesterol crystals with retained and aggregated LDL, and inflammatory activation will be
studied in various mouse models of atherosclerosis by optical microscopy.
Better understanding of the cellular and molecular events occurring in atherosclerotic lesions can lead
to better risk assessments and potentially new therapies.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
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批准号:9333438
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A JEM 1400 Electron Microscope for a Core Facility
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批准号:7793743
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A multiphoton microscope for translational and basic biomedical research
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批准号:7842170
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Macrophage-lipoprotein interactions
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批准号:7650897
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资助金额:$42.25万
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财政年份:2009
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负责人:Frederick R. Maxfield
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Macrophage-lipoprotein Interactions
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批准号:8185032
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资助金额:$42.25万
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财政年份:2009
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负责人:Frederick R. Maxfield
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依托单位:
Macrophage-lipoprotein Interactions
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批准号:9384099
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资助金额:$43.59万
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财政年份:2009
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负责人:Frederick R. Maxfield
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批准号:10117551
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资助金额:$36.42万
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负责人:Frederick R. Maxfield
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批准号:7860560
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资助金额:$42.25万
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负责人:Frederick R. Maxfield
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批准号:8299476
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资助金额:$42.25万
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负责人:Frederick R. Maxfield
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Macrophage-Lipoprotein Interactions
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批准号:10444272
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Plasma membrane cholesterol and monocyte /macrophage function
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财政年份:2007
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依托单位:
Intraneuronal Abeta accumulation: mechanism of pathogenesis
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批准号:7835702
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依托单位:
ESR STUDY OF BIOPHYSICAL EFFECTS OF CHOLESTEROL ON ER-PROTEIN FUNCTION
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批准号:7183054
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资助金额:$0.46万
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负责人:Frederick R. Maxfield
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依托单位:
EFFECT OF CHOLESTEROL ENRICHMENT ON ENDOPLASMIC RETICULUM MEMBRANES
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批准号:7183041
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资助金额:$0.46万
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负责人:Frederick R. Maxfield
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依托单位: