Animals and Instrumentation Core

动物和仪器核心

• 批准号: 10094226
• 负责人: Michael W. Brands
• 金额: $ 29.34万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10094226
• 关键词: Acute; Animal Model; Animals; Apoptosis Inhibitor; Blood Pressure; Blood flow; Blood specimen; Cardiac Output; Catheters; Chronic; Coupled; Coupling; Data; Data Collection; Data Science; Drug Delivery Systems; Electrolyte Balance; Elements; Equilibrium; Essential Hypertension; Excretory function; Experimental Models; Female; Goals; Hydrochlorothiazide; Hypertension; Inbred SHR Rats; Inbred WKY Rats; Inflammatory; Ketones; Link; Measurement; Measures; Metabolic; Molecular; Monitor; Mycotoxins; Natriuresis; Necrosis; Pattern; Play; Positioning Attribute; Protocols documentation; Quality Control; Randomized; Rattus; Regulation; Renal Blood Flow; Renal function; Research Personnel; Reserpine; Resistance; Role; Services; Sodium; Structure; Tail; Telemetry; Time; Tissue Sample; Vascular Diseases; Vascular resistance; animal data; artery infusion; aspartylglutamate; blood flow measurement; data acquisition; design; experimental study; flexibility; hemodynamics; human model; hypertension treatment; inhibitor/antagonist; instrumentation; kidney dysfunction; male; novel strategies; novel therapeutics; pressure; programs; renal artery; surgical service

ANIMALS AND INSTRUMENTATION CORE SUMMARY CB, BRANDS The SHR experimental model of human essential hypertension is being used in Projects 1, 2, and 3 to study the mechanisms for DAMP-induced hypertension and vascular and renal dysfunction. Accurate blood pressure measurement is a requirement. The unique expertise of the Core B investigators adds a range of capabilities that move the Animal Core well beyond a simple blood pressure-measuring service. Incorporating electrolyte balance measurements adds depth and richness to interpreting blood pressure, through information such as the pressure natriuresis relationship and transients in blood pressure and sodium excretion at initiation or termination of treatment. Coupling chronic blood flow measurement with blood pressure reveals changes in vascular resistance, which is the actual effector in the regulation of pressure and flow. Flexibility is important because blood pressure will be measured over acute and chronic time frames, will be needed for very large groups of animals, and may need to be accompanied by repeated arterial blood samples in the same animal over a period of days to weeks. Core B is structured to provide the highest degree of quality control and consistency in animal data used across all Projects. The overall goal of Core B is to provide the expertise, materials, and labor to measure and interpret arterial pressure, blood flow, vascular resistance, renal function, and metabolic balance chronically in rats, and to provide the platform for developing new approaches for delivery of experimental compounds or measuring systemic or regional hemodynamics as needed. In addition, Core B will be positioned together with Core C to effectively centralize and coordinate activities that are critical elements of several experiments. One key mechanism for accomplishing this is through the Core B Hub Experiment in Aim 1. That experiment will provide blood and tissue samples to all 3 Projects in coordination with Core C. The Hub Experiment was designed so that Core B would serve as a hard link between the 3 Projects: The chronic blood pressure data and tissue samples will be used by all Projects. A key feature of this approach is that these basic data used by all the projects will come from the exact same animals. In addition to the direct benefits on data collection, analysis, and interpretation, this function of Core B inherently facilitates networking and exchange of ideas and information between all components of the Program Project, over and above what they already are doing directly between projects. Thus, through this experiment and other activities, Core B as a whole indeed serves as a hub that is critical to all 3 "spokes" (Projects) in this PPG.

动物和仪器仪表核心概述CB，品牌 人类原发性高血压的自发性高血压实验模型正在项目1、2和3中用于研究 湿性高血压和血管、肾功能障碍的机制。准确的血压 测量是一项要求。Core B调查员的独特专业知识增加了一系列能力 这使Animal Core远远超出了简单的血压测量服务。加入电解液 平衡测量通过以下信息增加了血压解释的深度和丰富性 高血压患者入院时血压和钠排泄的瞬变及其与血压的关系 终止治疗。将慢性血流量测量与血压相结合揭示了 血管阻力，它是调节压力和流量的实际效应器。灵活性很重要 由于血压将在急性和慢性时间范围内测量，因此将需要非常大的 动物群体，可能需要在同一动物身上反复采集动脉血样本 从几天到几周的时间。核心B的结构旨在提供最高程度的质量控制和 在所有项目中使用动物数据的一致性。 核心B的总体目标是提供专业知识、材料和人力来测量和解释 大鼠的动脉压、血流量、血管阻力、肾功能和代谢平衡 提供平台以开发提供实验化合物或测量的新方法 根据需要进行全身或局部血流动力学检查。此外，核心B将与核心C一起定位为 有效地集中和协调作为几个实验的关键要素的活动。一键 实现这一点的机制是通过目标1中的Core B Hub实验。该实验将提供 血液和组织样本到所有3个项目与Core C协调。Hub实验是这样设计的 核心B将作为3个项目之间的硬链接：慢性血压数据和组织 所有项目都将使用样本。这种方法的一个关键特征是，所有 项目将来自完全相同的动物。除了在数据收集、分析、 和解释，核心B的这一功能本质上促进了联网和思想交流，并 计划项目的所有组件之间的信息，以及他们已经在做的事情 直接在项目之间。因此，通过这项实验和其他活动，核心B作为一个整体确实起到了 作为一个枢纽，这是关键的所有3个“辐条”(项目)在这个PPG。