Radical SAM Enzymes: Molecular Mechanisms of Radical Initiation

自由基 SAM 酶：自由基引发的分子机制

• 批准号: 10132741
• 负责人: Joan B Broderick
• 金额: $ 35.55万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10132741
• 关键词: Antibiotics; Biological; Biology; Carbon; Chemicals; Complex; DNA Repair; Disease; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Enzymes; Freezing; Goals; Human; Infection; Iron; Life; Metals; Microbe; Molecular; Organometallic Chemistry; Pathway interactions; Pharmacologic Substance; Process; Property; Reaction; Research; Structure; Sulfur; Work; X-Ray Crystallography; beneficial microorganism; catalyst; chemical reaction; cobamamide; cofactor; computer studies; insight; member; novel; pathogenic microbe; photolysis

Radical SAM is one of the largest enzyme superfamilies known, with its members catalyzing a remarkable diversity of reactions in all domains of life. Radical SAM enzymes are involved in the synthesis of essential cofactors and antibiotics, repair of DNA damage, and the assembly of complex biological metal clusters, among many other reactions. The presence of radical SAM enzymes in humans as well as in both beneficial and pathogenic microbes lends high significance to understanding their fundamental properties and mechanisms. The proposed research will develop a critical understanding of radical SAM mechanisms, in particular the radical initiation process common to all enzymes in this large and diverse superfamily. Research efforts will focus on trapping radical intermediates using rapid freeze- quench, cryoreduction, annealing, and photolysis approaches. These intermediates will then be characterized by using spectroscopic approaches such as electron paramagnetic resonance and electron- nuclear double resonance, and structural approaches such as X-ray crystallography. Computational studies will provide insights into stability/reactivity and reaction pathways. Much of the work will build on the recent discovery of a novel organometallic intermediate containing a direct bond between an iron of the iron-sulfur cluster and a carbon of an adenosyl moiety, a structure reminiscent of coenzyme B12. The results will provide an understanding of this fundamental and ubiquitous reaction in biology, and its surprising involvement of organometallic chemistry.

自由基SAM是已知的最大的酶超家族之一，其成员催化a