Miniaturized head-mounted device for pan-cortical electro-optical activity monitoring

项目摘要 本申请是根据生物工程研究资助的R 01机制提交的。这 一项申请提出开发一种小型化的头戴式设备， 光学成像和皮层脑电图（ECoG）记录，以实现整体的多模式活动映射 自由行为时的大脑皮层该提案建立在我们实验室的技术发展基础上-透明 聚合物头骨，提供光学访问估计800，000至1，000，000神经元跨越90平方毫米的 小鼠背侧皮质的表面。在AIM 1中，我们将开发头戴式成像系统， 自由活动和行为小鼠整个背侧皮层的中尺度活动映射。在AIM 2中，我们将 集成多层喷墨打印皮层脑电图（ECoG）的工程透明聚合物颅骨 电极阵列，允许同时光学成像和皮层表面场电位记录。我们将 集成到AIM 1开发的头戴式设备中的工程师定制接口电子设备， 在自由活动的小鼠中同步ECoG记录和皮质宽成像。在AIM 3中，我们将利用 在目标1和2中开发的设备，用于研究在新对象期间皮层活动模式的动态 识别和探索任务。这些发展将从根本上实现新的实验 在老鼠身上的研究，揭示了对神经元计算的新见解，这些神经元计算是感官知觉的基础， 行动和认知过程。

PROJECT SUMMARY This application is submitted in response to the Bioengineering Research Grant’s R01 mechanism. This application proposes to develop a miniaturized head-mounted device that seamlessly integrate wide-field optical imaging and electrocorticogram (ECoG) recordings to achieve multimodal activity mapping of the whole cortex during free behavior. The proposal builds on technological developments in our lab – transparent polymer skulls that provide optical access to an estimated 800,000 to 1,000,000 neurons across 90 mm2 of the surface of the dorsal cortex of the mouse. In AIM 1 we will develop head-mounted imaging systems for mesoscale activity mapping of the whole dorsal cortex in freely moving and behaving mice. In AIM 2, we will engineer transparent polymer skulls integrated with multilayer inkjet-printed electrocorticogram (ECoG) electrode arrays that allows simultaneous optical imaging and cortical surface field potential recordings. We will engineer custom interface electronics integrated into the head-mounted device developed in AIM 1 to enable simultaneous ECoG recordings and cortex wide imaging in freely moving mice. In AIM 3 we will utilize the devices developed in Aims 1 and 2, to study dynamics of cortical activity patterns during novel object recognition and exploration tasks. These developments will enable fundamentally new kinds of experimental studies in the mouse and reveal new insights into the neuronal computations that underlie sensory perception, action and cognitive processes.