3D Printed Multifunctional Brain Windows for Simultaneous Optical Imaging and Electrophysiology

项目总结 该提案响应了PAR-18-870题为“大脑计划：开发优化和验证 神经科学研究的新工具和新技术(STTR)。该项目是一个快速跟踪STTR与一个 第一阶段和第二阶段。小企业应用环球动力公司(AUD)正在与 明尼苏达大学(明尼苏达大学)数千万个神经元的活动模式--都被组织成电路 分布在多个大脑区域的大脑调节着我们与外部世界的互动。机械师 理解感官知觉、动作、情感和认知的神经元基础需要 测量这些神经元回路的活动，以单细胞分辨率跨越几毫米，并以多个 时间尺度，时间尺度。我们建议设计并商业化传播透明聚合物 同时允许宽视场光学神经传感和高时间分辨率电学的头骨 整个哺乳动物大脑皮质的录音。我们将在实验室开发的关键技术的基础上再接再厉。 具体地说，我们的合作伙伴开发了一种设计和制造透明头骨(大脑)的方法 Windows)，它允许对整个背侧皮质的结构和功能进行亚细胞分辨率成像 行为不端的老鼠。在这项建议的第一阶段，我们将首先利用三维打印方法来 使这些透明头骨具有8个通道的透明皮层脑电记录 电极(目标1)。这些设备将用于同时进行中尺度钙成像和 经社理事会记录(目标2)。在这项建议的第二阶段，我们会完善3D打印方法，以实现 超高密度、透明的128通道μ脑电地形图阵列(目标1)，用于同时进行全皮质脑电地形图和 细胞分辨率钙成像(AIM 2)。最后，我们将设计不同版本的技术，以允许 体表皮层脑电和全皮质光学成像同步细胞外记录实现平台 这使得真正的3D大脑活动地图(Aim 3)成为可能。

PROJECT SUMMARY This proposal responds to PAR-18-870 titled “BRAIN Initiative: Development Optimization, and Validation of Novel Tools and Technologies for Neuroscience Research (STTR).” This project is a Fast Track STTR with a phase I and phase II. The small business, Applied Universal Dynamics Corp. (AUD) is collaborating with the University of Minnesota (U of MN). The activity patterns of tens of millions of neurons – all organized into circuits distributed across multiple brain regions mediate our interaction with the outside world. A mechanistic understanding of the neuronal underpinnings of sensory perception, action, emotion, and cognition requires measuring activities of these neuronal circuits at single cell resolution across several millimeters, and at multiple temporal scales temporal scales. We propose to engineer and commercially disseminate transparent polymer skulls that simultaneously allow wide-field optical neural sensing along with high temporal resolution electrical recordings from the whole mammalian cortex. We will build on key technologies developed in in our laboratories. Specifically, our collaborative has developed a methodology to design and fabricate transparent skulls (Brain Windows) that allow sub-cellular resolution imaging of structure and function of the whole dorsal cortex in behaving mice. In PHASE I of this proposal, we will first utilize 3-dimensional printing methodologies to functionalize these transparent skulls with 8 channels of transparent electrocorticogram (ECoG) recording electrodes (Aim 1). These devices will be utilized to perform simultaneous mesoscale calcium imaging and ECoG recordings (Aim 2). In PHASE II of this proposal, we will refine the 3D printing methodology to realize ultra-high density, transparent 128 channel μECoG arrays (Aim 1) for simultaneous whole cortex ECoG and cellular resolution calcium imaging (Aim 2). Finally, we will engineer versions of the technology that allow simultaneous extracellular recordings with surface ECoG and whole cortex optical imaging to realize a platform that allows true 3D brain activity mapping (Aim 3).