Core D - Immunopathology

核心 D - 免疫病理学

• 批准号: 10239109
• 负责人: GEORGE F MURPHY
• 金额: $ 16.04万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10239109
• 关键词: Affect; Algorithmic Analysis; Antibodies; Archives; Autoimmune Diseases; Autoimmunity; BCL2 gene; Biological Specimen Banks; Body Weight decreased; CASP3 gene; CD8B1 gene; Cell physiology; Cells; Chronic; Classification; Cleaved cell; Clinical; Communities; Complement; Computer software; Data; Databases; Detection; Development; Epitopes; Evaluation; FOXP3 gene; Feedback; Formalin; Funding; Future; Genes; Goals; Histologic; Histology; ITGAM gene; ITGAX gene; Image; Image Analysis; Immunity; Immunofluorescence Immunologic; Immunologics; Individual; Investigation; Label; Learning; Lesion; Letters; Libraries; Machine Learning; Maintenance; Malignant Neoplasms; Mediator of activation protein; Molecular; Morphology; Mus; Mutant Strains Mice; NCAM1 gene; PTPRC gene; Paraffin Embedding; Pathology; Pathway interactions; Periodicity; Positioning Attribute; Protocols documentation; Research Personnel; Resolution; Sampling; Scanning; Severities; Site; Slide; Stains; Standardization; T-Lymphocyte; Technology; Tissue Sample; Tissues; Tumor Immunity; Update; Virus Diseases; Visualization; Woman; Work; antiviral immunity; base; biological specimen archives; chronic infection; congenic; deep learning; design; gene product; immunopathology; meetings; multiplexed imaging; prevent; programmed cell death protein 1; programs; prospective; quantitative imaging; receptor; success; synergism

CORE SUMMARY The overall goal of this PPG application is to develop a mechanistic understanding of the cellular and molecular basis for PD1 and LAG3 interactions that regulate T cell function in autoimmunity, cancer and chronic viral infections. Core D will provide sophisticated and relevant technologies and expertise to PPG investigators for understanding how the interplay between PD1 and LAG3 affects cellular interactions and relevant molecular pathways in intact experimental tissues from mice with autoimmune disease (Project 1), cancer (Project 2) and chronic viral infection (Project 3). The Aims of this Core are: AIM 1: To provide basic expertise and technologies for detailed immunopathologic tissue analysis; AIM 2: To apply high- resolution quantitative, correlative immunopathology using highly-multiplexed cyclic immunofluorescence (t- CyCIF); and AIM 3: To maintain an annotated biospecimen bank/archive for future studies. These data will interface with data generated by Core C and PPG projects in order to develop protocols to visualize new molecules, as well as metrics generated from conventional histology (e.g., qualitative assessment and grading of lesion severity) and clinical parameters (e.g. survival and weight loss). Core D will interact extensively with all three Projects, with Core A for oversight and administrative support, and Core C by developing new staining protocols for cellular and histologic investigation of key genes and their products that Core C determines are regulated by PD1 and LAG3.

核心摘要 该PPG应用的总体目标是开发对细胞和组织的机械理解。 PD 1和LAG 3相互作用的分子基础，调节自身免疫，癌症和慢性疾病中的T细胞功能 病毒感染核心D将为PPG研究者提供先进的相关技术和专业知识 了解PD 1和LAG 3之间的相互作用如何影响细胞相互作用 自身免疫性疾病小鼠完整实验组织中相关分子通路的研究 （项目1）、癌症（项目2）和慢性病毒感染（项目3）。本核心的目标是：目标1： 为详细的免疫病理组织分析提供基本的专业知识和技术;目标2：应用高 分辨率定量，相关免疫病理学使用高度多重循环免疫荧光（t- CyCIF）;和AIM 3：维护注释的生物标本库/档案，以供未来研究使用。这些数据将 与Core C和PPG项目生成的数据接口，以便制定方案， 分子以及由常规组织学产生的度量（例如，定性评价与分级 病变严重程度）和临床参数（例如存活率和体重减轻）。核心D将与 所有三个项目，核心A用于监督和行政支助，核心C用于开发新的染色剂， 核心C决定的关键基因及其产物的细胞和组织学研究方案是 由PD 1和LAG 3调节。