High-throughput Latent HIV Reservoir Assay Using Microwell Arrays

该项目的目标是设计一个高通量检测平台，可用于可重复地量化从接受高效抗逆转录病毒治疗的个体中分离的静息CD 4+记忆T细胞中具有复制能力的潜伏HIV库的大小变化。 包括通过证明与定量病毒生长试验（QVOA）和/或功能性非诱导HIV前病毒（使用从接受优化抗逆转录病毒治疗的病毒抑制HIV+个体中分离的细胞）的相关性来验证试验的研究。

The goal of this project is to design a high-throughput assay platform that can be used to reproducibly quantify changes in the size of the replication-competent latent HIV reservoir in resting CD4+ memory T cells isolated from individuals on highly effective antiretroviral therapy. Included are studies for validating the assay by demonstrating correlation with quantitative viral outgrowth assays (QVOA) and/or functional non-induced HIV proviruses using cells isolated from virally suppressed HIV+ individuals on optimized antiretroviral therapy.