PATHOLOGY PEER REVIEW AND PATHOLOGY SUPPORT FOR THE DNTP AND DIR AT NIEHS" CHARLES RIVER LABORATORIES (CRL)

NIEHS 查尔斯河实验室 (CRL) 的 DNTP 和 DIR 的病理学同行评审和病理学支持

• 批准号: 10241886
• 负责人: JAMES MORRISON
• 金额: $ 254.86万
• 依托单位: CHARLES RIVER LABORATORIES INTNTL, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-24 至 2021-08-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10241886
• 关键词: Adverse effects; Apoptosis; Autopsy; Cell Proliferation; Chemicals; Consult; Contracts; DNA; DNA sequencing; Data; Development; Electron Microscopy; Ensure; Environmental Exposure; Evaluation; Exposure to; Genotype; Goals; Government; Hazard Identification; Histology; Immunohistochemistry; In Situ; In Situ Hybridization; Journals; Laboratories; Laboratory Animal Medicine; Laboratory Animals; Malignant Neoplasms; Manuscripts; Molecular Biology; Molecular and Cellular Biology; Morphology; National Institute of Environmental Health Sciences; National Toxicology Program; Pathologic; Pathology; Peer Review; Persons; Preparation; Procedures; Public Health; Publications; RNA; Reporting; Research Design; Research Personnel; Rivers; Services; Site; Slide; Specimen; Stains; Support Contracts; Techniques; Time; Tissues; Toxic effect; Training; Work; base; carcinogenicity; developmental toxicity; disorder prevention; immunotoxicity; molecular pathology; posters; reproductive toxicity; research study; working group

The subject contract was established to provide a broad range of routine and specialized pathology services in support of studies conducted under contract by the National Toxicology Program (NTP) and for supplemental studies on pathology specimens generated through these studies. The contract also provides critical pathology (including molecular pathology) support for studies conducted by in-house investigators at NIEHS and other NIEHS contract studies. Specifically, the following services are supported by this contract: 1) necropsy and in-house necropsy assistance; 2) histology (tissue trimming and processing, slide preparation, and staining); 3) histopathological evaluation/interpretation; 4) application of specialized qualitative and quantitative morphological procedures (immunohistochemistry, morphometrics, cell proliferation, apoptosis, electron microscopy); 5) adaptation, development, refinement, application of new techniques in cellular and molecular biology including (but not limited to) genotyping, DNA sequencing, in situ hybridization, DNA and RNA isolation and amplification, and real time, quantitative, and in situ PCR, as well as training in the application of these techniques; 6) Pathology Peer Review (including Pathology Data Review [PDR], Audit of Pathology Specimens [APS], Quality Assessment of pathology evaluations and Pathology Working Group Coordination [QA/PWG]) and technical support for these activities; 7) pathology data entry; 8) presentation or publication of pathology data or techniques, or support for these activities; 9) on-site pathology support, and 10) administrative support for the laboratory animal medicine group. The following work was completed for the National Toxicology Program and the NIEHS on this contract in the past year: four PWGs; 2 histology projects, primary pathology evaluations for 3 immunotoxicity studies, pathological evaluation of 19 studies for in-house NIEHS projects, and preparation of 13 posters, journal publications, or other publications. In addition, there are many other projects for the NTP and NIEHS that are in progress. The support person performing tasks for the laboratory animal group has worked full-time for the year being reported.

签订主题合同的目的是提供广泛的常规和专业病理学服务，以支持国家毒理学计划 (NTP) 根据合同进行的研究以及对通过这些研究产生的病理标本的补充研究。该合同还为 NIEHS 内部研究人员进行的研究和其他 NIEHS 合同研究提供关键病理学（包括分子病理学）支持。具体来说，本合同支持以下服务： 1) 尸检和内部尸检协助； 2) 组织学（组织修剪和处理、玻片制备和染色）； 3）组织病理学评估/解释； 4）应用专门的定性和定量形态学程序（免疫组织化学、形态计量学、细胞增殖、凋亡、电子显微镜）； 5) 细胞和分子生物学新技术的适应、开发、完善和应用，包括（但不限于）基因分型、DNA测序、原位杂交、DNA和RNA分离和扩增，以及实时、定量和原位PCR，以及这些技术应用的培训； 6) 病理学同行评审（包括病理学数据评审[PDR]、病理标本审核[APS]、病理学评估质量评估和病理学工作组协调[QA/PWG]）以及对这些活动的技术支持； 7) 病理数据录入； 8) 病理数据或技术的呈现或出版，或对这些活动的支持； 9) 现场病理学支持，以及 10) 实验动物医学组的行政支持。去年，国家毒理学计划和 NIEHS 就该合同完成了以下工作：四个 PWG； 2 个组织学项目、3 项免疫毒性研究的主要病理学评估、内部 NIEHS 项目的 19 项研究的病理学评估，以及准备 13 份海报、期刊出版物或其他出版物。此外，NTP 和 NIEHS 还有许多其他项目正在进行中。为实验动物组执行任务的支持人员在报告的年份中一直全职工作。