PATHOLOGY PEER REVIEW AND PATHOLOGY SUPPORT FOR THE DNTP AND DIR AT NIEHS" CHARLES RIVER LABORATORIES (CRL)

该主题合同的建立是为了提供广泛的常规和专业病理学服务，以支持根据国家毒理学计划（NTP）的合同进行的研究，以及通过这些研究产生的病理标本的补充研究。该合同还为NIEHS和其他NIEHS合同研究的内部调查人员进行的研究提供关键病理学（包括分子病理学）支持。具体而言，本合同支持以下服务：1)尸检和内部尸检协助；2)组织学（组织修剪和处理、载玻片制备、染色）；3)组织病理学评价/解释；4)应用专门的定性和定量形态学程序（免疫组织化学、形态计量学、细胞增殖、细胞凋亡、电子显微镜）；5)细胞和分子生物学新技术的适应、发展、改进和应用，包括（但不限于）基因分型、DNA测序、原位杂交、DNA和RNA分离和扩增、实时、定量和原位PCR，以及这些技术应用方面的培训；6)病理同行评审（包括病理数据评审[PDR]、病理标本审核[APS]、病理评估质量评估和病理工作组协调[QA/PWG]）以及这些活动的技术支持；7)病理数据录入；8)病理数据或技术的展示或发表，或对这些活动的支持；9)现场病理支持，10)实验动物药物组的行政支持。在过去一年中，国家毒理学计划和NIEHS就该合同完成了以下工作：四个PWGs；2个组织学项目，3个免疫毒性研究的初步病理评估，19个内部NIEHS项目的病理评估，13个海报，期刊出版物或其他出版物的准备。此外，NTP和NIEHS还有许多其他项目正在进行中。为实验动物组执行任务的支持人员已在报告年度全职工作。

The subject contract was established to provide a broad range of routine and specialized pathology services in support of studies conducted under contract by the National Toxicology Program (NTP) and for supplemental studies on pathology specimens generated through these studies. The contract also provides critical pathology (including molecular pathology) support for studies conducted by in-house investigators at NIEHS and other NIEHS contract studies. Specifically, the following services are supported by this contract: 1) necropsy and in-house necropsy assistance; 2) histology (tissue trimming and processing, slide preparation, and staining); 3) histopathological evaluation/interpretation; 4) application of specialized qualitative and quantitative morphological procedures (immunohistochemistry, morphometrics, cell proliferation, apoptosis, electron microscopy); 5) adaptation, development, refinement, application of new techniques in cellular and molecular biology including (but not limited to) genotyping, DNA sequencing, in situ hybridization, DNA and RNA isolation and amplification, and real time, quantitative, and in situ PCR, as well as training in the application of these techniques; 6) Pathology Peer Review (including Pathology Data Review [PDR], Audit of Pathology Specimens [APS], Quality Assessment of pathology evaluations and Pathology Working Group Coordination [QA/PWG]) and technical support for these activities; 7) pathology data entry; 8) presentation or publication of pathology data or techniques, or support for these activities; 9) on-site pathology support, and 10) administrative support for the laboratory animal medicine group. The following work was completed for the National Toxicology Program and the NIEHS on this contract in the past year: four PWGs; 2 histology projects, primary pathology evaluations for 3 immunotoxicity studies, pathological evaluation of 19 studies for in-house NIEHS projects, and preparation of 13 posters, journal publications, or other publications. In addition, there are many other projects for the NTP and NIEHS that are in progress. The support person performing tasks for the laboratory animal group has worked full-time for the year being reported.