Core 2 - Crystallography

核心2：结晶学-摘要 CRNA晶体学核心将与CRNA调查人员合作解决 与HIV相关的RNA-蛋白质复合体、RNA和蛋白质的晶体结构 调解的过程。核心还提供基本的专业知识和材料， 远远超出了结晶学的任务，并支持许多大分子 CRNA研究的准备和分析方面。核心团队，由史密斯和 Stuckey提供为RNA和RNA量身定做的分子生物学方面的协作专业知识- 蛋白质复合体，包括结构设计、表达测试和开发 净化方案。所得到的载体、表达质粒和方案 作为纯化的RNA，RNA-蛋白质复合体和蛋白质被提供给合作 CRNA的研究人员，密歇根大学的核心实验室担任 材料和协议的储存库。结晶学核心的两个新成员 团队、CRNA研究员Pornillos(弗吉尼亚大学)和重要贡献者张 (NIDDK，NIH)，带来了在设计、生产和验证方面的非凡专业知识 RNA和RNA-蛋白质复合体，特别是用于结晶。在CRNA 2.0中，核心 将开发新的RNA表达方法，形成RNA-蛋白质复合体 通过共表达和设计伴侣蛋白来帮助RNA结晶。核心意志 密歇根大学也建立了体外RNA合成能力。以支持智能交通运输系统 结晶学任务，核心将开发尖端方法来帮助RNA 晶化，提高晶体的衍射质量，解决晶相问题 对于含RNA晶体，提高含RNA晶体的结晶精确度 结构，并促进结晶学和电子显微镜的结合。

Core 2: Crystallography - Summary The CRNA Crystallography Core will collaborate with CRNA investigators to solve crystal structures of RNA-protein complexes, RNAs and proteins relevant to HIV RNA- mediated processes. The Core also provides essential expertise and materials that extend far beyond the crystallography mission and support many macromolecule preparation and analysis aspects of CRNA research. The Core team, led by Smith and Stuckey, offers collaborative expertise in molecular biology tailored for RNAs and RNA- protein complexes, including construct design, expression testing, and development of purification protocols. The resulting vectors, expression plasmids and protocols as well as purified RNAs, RNA-protein complexes and proteins are provided to collaborating investigators in the CRNA, with the Core lab at the University of Michigan acting as a repository for materials and protocols. Two new members of the Crystallography Core team, CRNA investigator Pornillos (U Virginia) and Significant Contributor Zhang (NIDDK, NIH), bring exceptional expertise in the design, production and validation of RNAs and RNA-protein complexes, particularly for crystallization. In CRNA 2.0, the Core will develop novel methods for expression of RNAs, formation of RNA-protein complexes by co-expression, and design of chaperones to aid RNA crystallization. The Core will also establish an in vitro RNA synthesis capability at Michigan. In support of its crystallography mission, the Core will develop cutting-edge methods to aid RNA crystallization, to improve the diffraction quality of crystals, to solve the phase problem for RNA-containing crystals, to improve crystallographic refinement for RNA-containing structures, and to facilitate the combination of crystallography and electron microscopy.