Core 2 - Crystallography

核心 2 - 晶体学

• 批准号: 10245109
• 负责人: JANET L. SMITH
• 金额: $ 36.25万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-17 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10245109
• 关键词: Cell Line; Complex; Crystallization; Crystallography; Development; Electron Microscopy; Genetic Transcription; HIV; In Vitro; Mediating; Methods; Michigan; Mission; Molecular Biology; Molecular Chaperones; National Institute of Diabetes and Digestive and Kidney Diseases; Phase; Plasmids; Preparation; Process; Production; Proteins; Protocols documentation; RNA; RNA chemical synthesis; Research Personnel; Resolution; Structure; Testing; United States National Institutes of Health; Universities; Validation; Virginia; design; design and construction; improved; macromolecule; member; novel; protein complex; repository; research study; vector

Core 2: Crystallography - Summary The CRNA Crystallography Core will collaborate with CRNA investigators to solve crystal structures of RNA-protein complexes, RNAs and proteins relevant to HIV RNA- mediated processes. The Core also provides essential expertise and materials that extend far beyond the crystallography mission and support many macromolecule preparation and analysis aspects of CRNA research. The Core team, led by Smith and Stuckey, offers collaborative expertise in molecular biology tailored for RNAs and RNA- protein complexes, including construct design, expression testing, and development of purification protocols. The resulting vectors, expression plasmids and protocols as well as purified RNAs, RNA-protein complexes and proteins are provided to collaborating investigators in the CRNA, with the Core lab at the University of Michigan acting as a repository for materials and protocols. Two new members of the Crystallography Core team, CRNA investigator Pornillos (U Virginia) and Significant Contributor Zhang (NIDDK, NIH), bring exceptional expertise in the design, production and validation of RNAs and RNA-protein complexes, particularly for crystallization. In CRNA 2.0, the Core will develop novel methods for expression of RNAs, formation of RNA-protein complexes by co-expression, and design of chaperones to aid RNA crystallization. The Core will also establish an in vitro RNA synthesis capability at Michigan. In support of its crystallography mission, the Core will develop cutting-edge methods to aid RNA crystallization, to improve the diffraction quality of crystals, to solve the phase problem for RNA-containing crystals, to improve crystallographic refinement for RNA-containing structures, and to facilitate the combination of crystallography and electron microscopy.

核心2：晶体学-总结 CRNA晶体学核心将与CRNA研究人员合作， RNA-蛋白质复合物、RNA和与HIV RNA相关的蛋白质的晶体结构- 中介过程。核心还提供必要的专业知识和材料， 远远超出了晶体学的使命，并支持许多大分子 CRNA研究的制备和分析方面。由史密斯领导的核心团队， 斯塔基，提供合作的专业知识，在分子生物学量身定制的RNA和RNA- 蛋白质复合物，包括构建设计，表达测试和开发 纯化方案。所得载体、表达质粒和方案以及 作为纯化的RNA、RNA-蛋白质复合物和蛋白质， CRNA的研究人员，密歇根大学的核心实验室作为一个 材料和协议的存储库。晶体学核心的两个新成员 团队，CRNA研究员Pornillos（弗吉尼亚大学）和重要贡献者Zhang （NIDDK，NIH），在设计，生产和验证方面提供卓越的专业知识， RNA和RNA-蛋白质复合物，特别是用于结晶。在CRNA 2.0中，核心 将开发新的方法来表达RNA，形成RNA-蛋白质复合物 通过共表达和设计分子伴侣来帮助RNA结晶。芯会 还在密歇根州建立了体外RNA合成能力。为支持其 作为晶体学使命，核心将开发尖端方法来帮助RNA 结晶，改善晶体衍射质量，解决相位问题 对于含RNA的晶体，以改善含RNA的晶体的晶体学细化， 结构，并促进晶体学和电子显微镜的结合。