Synthetic Chemical Biology

合成化学生物学

• 批准号: 10245047
• 负责人: BLAKE PETERSON
• 金额: $ 16.16万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-15 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10245047
• 关键词: Address; Animal Model; Anti-Infective Agents; Biochemistry; Biological; Biological Assay; Biology; Biomedical Research; Biotin; Cell Culture Techniques; Cell model; Cells; Cellular Assay; Centers of Research Excellence; Chemicals; Chemistry; Client; Communicable Diseases; Communities; Core Facility; Custom; DNA Sequencing Facility; Disease Pathway; Equipment; Evaluation; Faculty; Foundations; Funding; Generations; Genes; Goals; Grant; Human Resources; Imaging Device; In Vitro; Infrastructure; Kansas; Label; Lead; Methods; Microfabrication; Microfluidics; Microscopy; Mission; Modeling; Molecular; Molecular Analysis; Molecular Probes; National Institute of General Medical Sciences; Organic Chemistry; Peptides; Pharmaceutical Chemistry; Phase; Property; Research Personnel; Resolution; Resources; Rodent; Services; Structure; Tracer; Universities; Work; Zebrafish; biological systems; cellular imaging; chemical synthesis; design; drug discovery; experience; fluorescence imaging; fluorophore; genome sequencing; high resolution imaging; high throughput screening; improved; in vivo; novel; operation; outreach; peptidomimetics; programs; small molecule; success

PROJECT SUMMARY The Synthetic Chemical Biology Core (SCBC) was established in July 2016 from the merger of the Center for Molecular Analysis of Disease Pathways (CMADP) Molecular Probes Core (MPC) with the newly established Chemical Biology of Infectious Disease (CBID) Medicinal Chemistry Core (MCC). The synthetic capabilities of the SCBC will fulfill the specific aims of both COBRE centers. The mission for the SCBC in Phase II is to provide the junior faculty affiliated with CMADP, as well as other investigators in the state of Kansas, with novel molecular probes of biological systems. The SCBC will also offer the capability to evaluate these probes in vitro in whole- cell bioassays, and in vivo in zebrafish, an increasingly important model organism for biomedical research. The SCBC will also fulfill CBID’s missions concerning anti-infective drug discovery. These projects will be funded through the CBID. The SCBC Director is responsible for coordinating all SCBC Core lab activities to both COBRE centers. The SCBC will offer expert design of molecular probes and synthesis of both small molecules and peptides, with an emphasis on the generation of fluorescent and other tagged molecules. SCBC will also offer bioassays of molecular probes, including whole cell assays and assays using zebrafish, to bridge a gap that currently exists between very high resolution imaging of cells in culture and low resolution imaging of fluorescent tracers in rodents. The newly established SCBC would function synergistically with other CMADP-funded Core facilities, including the Genome Sequencing Core, to identify genes that impact functions of probes in vivo, and the Microfabrication and Microfluidics Core, to develop devices for imaging and chemical analysis. This integration is designed to empower faculty to use chemical biology approaches to investigate biological problems. The SCBC provides investigators with access to highly qualified personnel with experience in synthetic organic chemistry, biochemistry, microscopy, and approaches for optimization of biological properties of synthetic molecules. The Center has two Co-Core Leaders from the Department of Medicinal Chemistry at KU. Prof. Blake Peterson will continue to be the Leader of the CMADP’s part of the SCBC. Prof. Tom Prisinzano is the Leader of the CBID part of the SCBC. The Director of SCBC is Dr. Chamani Perera. The specific aims that will be addressed by the SCBC for this Phase II proposal are: (1) to continue to integrate resources from the proposed COBRE Center for Molecular Analysis of Disease Pathways Phase II with the existing COBRE Center for Chemical Biology of Infectious Disease Phase I to further establish the new SCBC, gain economies of scale, and serve faculty affiliated with the COBRE CMADP; (2) design and synthesize molecular probes of biological systems for faculty investigators; (3) enable faculty clients to conduct bioassays in cell culture and zebrafish models; and (4) provide outreach and administrative support necessary to manage Core activities, and offer services to the larger biomedical research community in the state of Kansas.

项目摘要 合成化学生物学核心（SCBC）于2016年7月从合并中心合并 与新建立的疾病途径（CMADP）分子探针核心（MPC）的分子分析 传染病（CBID）药物化学核心（MCC）的化学生物学。合成功能 SCBC将满足两个山核桃中心的具体目标。第二阶段SCBC的任务是提供 初级教职员工与CMADP以及堪萨斯州的其他研究人员，具有新颖的分子 生物系统的探针。 SCBC还将提供整体评估这些探针的能力 细胞生物测定和斑马鱼的体内，这是一种越来越重要的生物医学研究模型。 SCBC还将履行有关抗感染药物发现的CBID任务。这些项目将被资助 通过CBID。 SCBC主管负责协调所有SCBC核心实验室活动 中心。 SCBC将提供分子问题的专家设计以及小分子的合成 肽，重点是荧光和其他标记分子的产生。 SCBC也将提供 分子问题的生物测定，包括使用斑马鱼的全细胞测定和测定，以弥合差距 目前存在于培养细胞的非常高的分辨率成像与荧光的低分辨率成像之间 啮齿动物的示踪剂。新建立的SCBC将与其他CMADP资助的核心协同起作用 设施，包括基因组测序核心，以识别影响体内问题功能的基因，以及 微结构和微流体核心，开发用于成像和化学分析的设备。这 整合旨在授权教师使用化学生物学方法来研究生物学 问题。 SCBC为调查人员提供了具有合成有机物经验的高素质人员 化学，生物化学，显微镜和合成生物学特性的方法 分子。该中心有两个来自KU药物化学系的联合核心领导人。布雷克教授 彼得森将继续成为CMADP SCBC中的领导者。汤姆·普里斯汀扎诺教授是领导者 SCBC的CBID部分。 SCBC的主任是Chamani Perera博士。具体目标 SCBC针对此第二阶段提案的介绍是：（1）继续整合提案中的资源 疾病途径分子分析的毛线II期分子分析中心与现有的毛关系中心 感染疾病的化学生物学一阶段，以进一步建立新的SCBC，增长规模经济， 并为CMADP的教职员工服务； （2）设计和合成生物学的分子问题 教师调查员的系统； （3）使教师能够在细胞培养和斑马鱼中进行生物测定 模型； （4）提供管理核心活动所必需的外展和行政支持，并提供 为堪萨斯州大型生物医学研究界提供服务。