Subcortical Neural Coding and Dynamics

皮层下神经编码和动力学

• 批准号: 10247572
• 负责人: Ilana Witten
• 金额: $ 40.44万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-28 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10247572
• 关键词: Address; Anatomy; Area; Behavior; Biophysics; Brain; Calcium; Calcium Signaling; Cannulas; Cerebellum; Code; Cognition; Collaborations; Complex; Computer Models; Corpus striatum structure; DRD2 gene; Data; Decision Making; Dendrites; Dopamine; Dorsal; Electrodes; Hippocampus (Brain); Image; Implant; Individual; Maps; Measures; Mind; Modeling; Neocortex; Neurons; Output; Pharmacology; Photons; Population; Process; Prosencephalon; Purkinje Cells; Pyramidal Cells; Ramp; Research Project Grants; Resolution; Rewards; Rodent; Sensory; Short-Term Memory; Signal Transduction; Silicon; Site; Substantia nigra structure; Testing; Time; Training; Ventral Tegmental Area; Viral; base; cognitive ability; cognitive process; cognitive task; dopaminergic neuron; experimental study; granule cell; insight; lens; millisecond; neural circuit; neural correlate; neuromechanism; new technology; novel; relating to nervous system; temporal measurement; two-photon; virtual; way finding

Project Summary: Project 3, Neural Coding and Dynamics—Subcortical Regions Working memory, the ability to temporarily hold multiple pieces of information in mind for manipulation, is central to virtually all cognitive abilities. This multi-component research project aims to comprehensively dissect the neural circuit mechanisms of this ability across multiple brain areas. The individual parts of the project cohere conceptually, in part, because they all involve rodents trained to perform a type of decision-making task that is based on the gradual accumulation of sensory evidence and thus relies on working memory. Although most previous characterization of neural correlates of working memory and decision making has focused on cortical regions, there is growing appreciation that subcortical regions contribute to these processes as well. Thus, this project focuses on characterization of the neural dynamics underlying working memory and decision-making in a network of subcortical regions. Specifically, cellular-resolution two- and three-photon calcium imaging will be used to characterize neural coding and dynamics in dopamine neurons in the ventral tegmental area and substantial nigra, granule cells and Purkinje cells of the cerebellum, medium spiny neurons in the striatum, and pyramidal cells in the hippocampus. This data will be supplemented with multi-electrode recordings, which capture fast neural activity that calcium imaging cannot. The experiments will combine these two data types for the first time in the setting of a working memory and decision-making task. The results from this project, together with those from another component that investigates similar measures in neocortex, are expected to provide an unprecedented amount of data that will give new insight into the processing of task-relevant information during a cognitive task across a wide variety of cortical and subcortical areas. Another component will combine these results with temporally and spatially specific inactivation data from the other two components to build and constrain a biophysically realistic, multi-region computational model of the behavior.

项目摘要：项目3，神经编码和动力学-皮质下区域 工作记忆，即在脑海中暂时记住多个信息以进行操作的能力，是 几乎是所有认知能力的核心。这一多组分研究项目旨在全面 剖析这种能力在多个大脑区域的神经回路机制。的各个部分 项目概念上的一致性，部分是因为它们都涉及到训练成执行一种类型的 基于感官证据的逐渐积累的决策任务，因此依赖于 工作记忆。尽管大多数先前对工作记忆与神经功能的关系的描述 决策的重点是皮质区域，人们越来越认识到皮质下区域 也为这些进程做出贡献。因此，本项目的重点是神经动力学的表征 大脑皮质下区域网络中潜在的工作记忆和决策。具体来说， 细胞分辨率双光子和三光子钙成像将用于表征神经编码和 腹侧被盖区和实质黑质、颗粒细胞和浦肯野的多巴胺能神经元的动态变化 小脑细胞，纹状体中的中等刺神经元，以及海马区的锥体细胞。这 数据将由多电极记录补充，这些记录捕捉到比钙更快的神经活动 成像则不能。实验将首次将这两种数据类型组合在 工作记忆和决策任务。这个项目的结果，以及另一个项目的结果 在大脑皮层研究类似措施的组件，预计将提供前所未有的 对认知过程中任务相关信息的处理提供新见解的数据量 任务涉及广泛的皮质和皮质下区域。另一个组件将合并这些结果 利用来自其他两个组件的时间和空间特定的停用数据来构建和约束 一种生物物理上真实的、多区域的行为计算模型。