Core C: Product Analysis Core

摘要：WNY风味烟草产品研究中心(CROFT)专注于 烟草制品中使用的调味料的毒性，特别关注电子尼古丁输送系统 (结束)。调味剂是用于终端产品、水烟的再灌装溶液的重要组成部分 还有调味雪茄。为此，Croft内的大多数项目都需要关于以下内容和/或排放量的数据 产品中的调味品。产品分析核心(PAC)将及时提供有关化学成分的数据 将在Croft内测试的产品，并将通知项目有关相对受欢迎的产品和产品 特定产品和口味的变化。PAC核心的基本目标是集中生产 与产品功能相关的数据，然后可以为Croft项目提供支持，帮助改进研究 效率。我们计划实现以下三个高度整合的具体目标： 目标1：确定使用的调味料及其在末端灌装溶液、水烟产品中的浓度 还有调味雪茄。我们提出了一种用于香料化学物质鉴定和定量的方法。 烟草制品采用GC-MS和GC-Q-TOF-MS方法。烟草产品的检测也将涉及 识别致癌、致突变、呼吸道过敏原、致敏剂和 刺激物。我们还将确定调味品的产品间和产品内的可变性，并确定产品内和产品内的可变性 调味品的品牌间差异。 目标2：确定末端气雾剂和水管产生的烟雾的香料产量和吸入剂量 烟草产品和调味雪茄。在目标1中确定了烟草产品中的调味品后，我们将 使用吸烟机测量实验室环境中产生的气雾剂、烟雾或蒸气中的调味品产量。 我们还将测试生成的气雾剂的潜在反应和调味品的热分解产物。在……里面 为了确保符合GLP标准，所有测试都将在完全符合的质量体系中进行。 目标3：使用二次数据分析监测加香烟草产品市场份额的趋势。此外 为了监测产品本身的特性，我们将利用市场扫描仪数据和路径数据库 确定烟头和其他烟草产品以及最畅销的香料烟头中最常见的味道 品牌。监管科学成果：除了提供项目1-4的相关数据外，我们还将 开发一种评估香料毒理学风险的方法，该方法考虑到香料的类型和 烟草制品和气溶胶/烟雾/蒸气中调味剂的浓度，识别任何 潜在的热击穿和反应产物，以及呼吸风险评估。这一方法将 有助于识别可能使化合物不适合吸入的任何特定吸入问题的证据 在烟草制品中使用。帐目委员会所产生的结果可协助制订适当的产品标准。 并对烟草产品中的调味品实施强有力的质量管理体系。

SUMMARY: The WNY Center for Research on Flavored Tobacco Products (CRoFT) is focused on the toxicity of flavorings used in tobacco products, with a particular focus on electronic nicotine delivery systems (ENDS). Flavoring chemicals are an essential part of refill solutions used in ENDS products, waterpipe tobacco and flavored cigars. To this end, most projects within CRoFT require data on the contents and/or emissions of flavorings in products. The Product Analysis Core (PAC) will provide timely data on the chemical constituents of products to be tested within the CRoFT and will inform projects about the relative popularity and product variation of particular products and flavors. The underlying goal of the PAC core is to centralize the production of data related to product features that can then feed the CRoFT projects, helping to improve research efficiency. We plan the following three highly integrated Specific Aims: AIM 1: Determine flavorings used and their concentrations in ENDS refill solutions, waterpipe tobacco products and flavored cigars. We propose an approach to the identification and quantitation of flavoring chemicals in tobacco products using GC-MS and GC-Q-TOF-MS methods. The testing of tobacco product will also involve identifying flavoring ingredients that are carcinogenic, mutagenic, respiratory allergens, sensitizers and irritants. We also will determine inter-product and intra-product variability in flavorings and determine intra- and inter-brand variability in flavorings. AIM 2: Determine flavoring yields and inhaled doses from ENDS aerosol and smoke generated from waterpipe tobacco products and flavored cigars. After identification of flavorings in tobacco products in Aim 1, we will measure flavoring yields in aerosol, smoke or vapor generated in laboratory settings using smoking machines. We will also test generated aerosols for potential reaction and thermal breakdown products of flavorings. In order to ensure compliance to the GLP standards, all tests will be done in a fully compliant quality system. AIM 3: Monitoring trends in flavored tobacco product market share using secondary data analysis. In addition to monitoring characteristics of products themselves, we will utilize market scanner data and PATH database to identify the most common flavors in ENDS and other tobacco products and top-selling flavored ENDS brands. Outcomes for Regulatory Science: In addition to providing relevant data for Projects 1-4, we will develop an approach for toxicological risk assessment of flavors that takes into account the type and concentration of flavoring chemicals in the tobacco products and aerosol/smoke/vapor, identification of any potential thermal breakdown and reaction products, and respiratory risk assessment. This approach will contribute to identifying any evidence of inhalation-specific issues that might make a compound unsuitable for use in tobacco products. Results generated by the PAC can help to develop appropriate product standards and implement robust quality management systems for flavorings in tobacco products.