Core 4: Animal Breeding (UAB) and Exposure Core (MRIGLOBAL)

核心 4：动物育种 (UAB) 和暴露核心 (MRIGLOBAL)

• 批准号: 10249112
• 负责人: Mohammad Athar
• 金额: $ 57.52万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10249112
• 关键词: Animals; Antidotes; Arsenicals; Attenuated; Back; Bar Codes; Body Fluids; Centers of Research Excellence; Chemical Agents; Chemical Warfare Agents; Chemicals; Cutaneous; Data; Data Collection; Evaluation; Exposure to; Flowcharts; Funding; Grant; Housing; In Vitro; Kidney; Laboratories; Liquid substance; Lung; Modeling; Molecular Target; Nitrogen; Oxides; Procedures; Protocols documentation; Running; Safety; Sampling; Schedule; Scientist; Services; Ships; Skin; Techniques; Temperature; Testing; Time; Tissue Harvesting; Tissues; Toxic effect; Visit; Work; animal breeding; animal colony; biobank; chemical threat; drug development; drug discovery; experience; experimental study; healing; in vivo; novel; operation; programs; prototype; renal damage; screening; skin damage; skin lesion; success; toxic industrial chemical

The major objectives of the Animal and Exposure Core-4 of the UAB Research Center of Excellence in Arsenicals are to support all scientific Projects and Drug Discovery and Development Core-3, as the success of the studies proposed within this Center application depend on the availability of sufficient animals in a timely manner, as well as the timely coordination with MRIGlobal for animal exposure to arsenicals. MRIGlobal has more than 60 years of corporate experience in management and operations of chemical agent facilities and associated projects. We bring to CACT-funded programs, proficiencies in small and large animal chemical agent models, test and evaluation, safe handling of chemical warfare agent (CWA) compounds, compounds with extreme toxicity, toxic industrial chemicals (TIC), and emerging chemical threats. Core-4 will generate a colony of animals of the desired size by assessing the requirement of each Project in the beginning of each year. Animals will be transported to MRIGlobal on a predefined time schedule. Once the animals have been exposed to arsenicals and their initial gross data pertaining to skin damage recorded, the animals will be euthanized by the staff at MRIGlobal and the harvested tissues and body fluids will be transported back to UAB. In addition, MRIGlobal will synthesize and store the arsenicals used for these animal studies for which they have facilities which approved by appropriate agencies. Core-4 will establish a biorepository on the UAB campus in which to store the returned tissue and body fluid samples as well as other samples generated directly by the Projects. Samples will be barcoded and banked in our laboratory. In addition, Core-4 will conduct the in vivo screening of agents that are synthesized by Drug Discovery and Development Core-3 and found to be highly effective in attenuating molecular targets in vitro. Accordingly, three specific aims of this core include: 1: To breed and ship animals for arsenicals exposure at MRIGlobal under approved animal protocols of UAB and MRIGlobal; 2: Coordinate with MRIGlobal for animal exposure to arsenicals, initial gross skin data collection, and return shipment to UAB of samples or healed animals for proper banking and distribution or further toxicity assessment, respectively; and 3: To perform in vivo screening of novel agents developed in Drug Discovery and Development Core-3.

UAB研究中心的动物和暴露核心4的主要目标 富裕砷的卓越将支持所有科学项目和药物发现以及 开发Core-3，作为该中心应用程序中提出的研究的成功 取决于及时的及时及时的可用性 与Mriglobal的协调，以动物暴露于砷中。 Mriglobal拥有60多个 在化学代理设施的管理和运营方面的公司经验多年 相关项目。我们带来了由CACT资助的计划，小动物和大型动物的经验 化学代理模型，测试和评估，化学战剂的安全处理（CWA） 化合物，具有极端毒性的化合物，有毒工业化学品（TIC）和新兴的化合物 化学威胁。 Core-4将通过评估来产生所需大小的动物菌落 每年年初的每个项目的要求。动物将被运送到 Mriglobal在预定义的时间表上。一旦动物暴露于砷 以及它们最初的总数据与记录的皮肤损伤有关，这些动物将被安乐死 Mriglobal和收获的组织和体液的工作人员将被运回UAB。 此外，Mriglobal将合成并存储用于这些动物研究的砷 他们拥有适当机构批准的设施。 Core-4将建立一个 UAB校园上的生物座位，在该校园中存储返回的组织和体液样品作为 以及其他项目直接生成的样本。样品将进行条形码和库存 在我们的实验室。此外，Core-4将进行体内筛选，对 通过药物发现和开发核心3合成，发现在 在体外衰减分子靶标。因此，该核心的三个具体目标包括：1： 根据批准的动物方案，在Mriglobal繁殖动物和船舶动物 UAB和Mriglobal; 2：与Mriglobal协调动物接触砷，初始 收集皮肤数据，然后将运送到UAB样品或治愈动物的货物中 银行和分配或进一步的毒性评估；和3：在体内表演 对药物发现和发育中开发的新型药物的筛查。