Multifunctional Nanoplatforms-Based Capture and identification of Circulating Tumor Biomarkers for Early Diagnosis of Lung Cancer

基于多功能纳米平台的循环肿瘤生物标志物的捕获和鉴定用于肺癌的早期诊断

• 批准号: 10256728
• 负责人: Paresh Chandra Ray
• 金额: $ 18.87万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-08 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10256728
• 关键词: Affect; African American; American Cancer Society; Antibodies; Bacteria; Biological Assay; Blood; Blood Screening; Blood specimen; CD81 gene; CTAG1 gene; Cancer Detection; Cancer Etiology; Cancer Patient; Cancer cell line; Carbon; Cells; Centers for Disease Control and Prevention (U.S.); Cessation of life; Clinical; Detection; Development; Devices; Diagnosis; Diagnostic; Disease; Early Diagnosis; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor Receptor; Epithelial; Ethnic group; Flow Cytometry; Fluorescence; Genes; Goals; Image; Imaging Techniques; Incidence; Magnetism; Malignant Neoplasms; Malignant neoplasm of lung; Mediator of activation protein; Medical center; Mesenchymal; Methods; Mississippi; National Cancer Institute; Neoplasm Circulating Cells; Neoplasm Metastasis; Outcome; Pathologist; Patients; Pharmaceutical Preparations; Population; Population Heterogeneity; Process; Proteins; Race; Reporting; Research; Testing; Time; Tumor Markers; Tumor-Derived; United States; Universities; Viola; Vision; Whole Blood; Work; base; blood-based biomarker; cancer biomarkers; cancer cell; cancer diagnosis; circulating biomarkers; clinical application; clinically relevant; design; detection assay; disease diagnosis; ethnic diversity; exosome; experimental study; graphene; health disparity; healthy volunteer; improved; lung cancer cell; mortality; nanomagnetic; nanoparticle; nanotechnology platform; novel; peripheral blood; racial diversity

Significant disparities in incidence and mortality rates for lung cancer are reported among ethnically and racially diverse population. African Americans suffer from lung cancer more than any other population. High mortality rate in lung cancer is due to being diagnosed at a later stage, where treatment options are limited. Our long-term goal is to lay the groundwork for developing novel nanoplatform based device which will provide a more accurate determination of the circulating cancer biomarkers from lung cancer patients in blood screening facilities. The objective of this proposal is to design magnetic-fluorescence multifunctional nanoplatform based device to capture, isolate and detect circulating tumor cells (CTCs) and tumor derived exosomes from blood samples in lung cancer patients and to establish correlations of these findings with survival. Our central hypothesis is that bio-conjugated multicolor fluorescence carbon dots attached magnetic nano-platform can be used to capture multiple types of CTCs and circulating exosomes from blood samples, and as a facile multicolor fluorescence probe for accurate identification of circulating biomarkers. The central hypothesis will be tested by pursuing two specific aims: 1) Developing fluorescence- magnetic nanoplatform for capture, and identification of multiple types of CTCs from lung cancer patient blood and 2) Capture and identification of tumor derived exosomes using nanoplatform and demonstration of clinical applications. The proposed research is significant, because with high sensitivity and real-time detection possibility, CDs attached magnetic nano-platform will be highly promising for detection and identification of several subpopulation types of CTCs and exosomes. The proximate expected outcome of this work is improving the capability of identifying multiple clinically relevant blood based biomarkers in blood screening facilities.

据报道，肺癌的发病率和死亡率存在显著差异 在不同民族和种族的人口中。非裔美国人患肺 癌症比其他任何人都多。肺癌的高死亡率是由于 在治疗选择有限的后期阶段被诊断出来。我们的长期 目标是为开发基于纳米平台的新型器件奠定基础， 将提供更准确的循环癌症生物标志物的测定， 肺癌患者在血液筛查设施。这项建议的目的是 设计基于磁荧光多功能纳米平台的装置， 分离和检测来自于以下细胞循环肿瘤细胞（CTC）和肿瘤来源的外来体 肺癌患者的血液样本，并建立这些发现的相关性 与生存。我们的中心假设是生物共轭荧光 碳量子点附着的磁性纳米平台可用于捕获多种类型 CTC和循环外泌体的血液样本，并作为一种简单的方法， 用于准确识别循环生物标志物的荧光探针。中央 将通过追求两个具体目标来测试假设：1）开发荧光- 用于捕获和鉴定多种类型的CTC的磁性纳米平台， 肺癌患者血液和2）捕获和鉴定肿瘤来源的 外泌体使用纳米平台和临床应用的演示。的 由于具有高灵敏度和实时性， 检测的可能性，光盘附着的磁性纳米平台将是非常有前途的 用于检测和鉴定CTC的几种亚群类型， 外来体这项工作的最接近的预期结果是提高能力 在血液筛查中识别多种临床相关的基于血液的生物标志物 设施