RAMAN AND HYPER RAYLEIGH SPECTROSCOPY OF NANOSCALE BIOLOGICAL INTERFACE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have demonstrated for the first time that the hyper¿Rayleigh Scattering (HRS), which has emerged over the past decade as a powerful method to determine the microscopic non-linear optical (NLO) properties of species in solution, could achieve the ultra-sensitive detection of single base-pair mismatch in oligonucleotide strands without any covalent tagging of dyes. We have also reported 2 laser induced fluorescence (LIF) studies of ss-DNA hybridization based on fluorescence quenching by gold nanoparticles through a distant dependent process. We have shown that the fluorescence of ss-DNA increases by a factor of 100 when it binds to a complementary DNA while the addition of single-base mismatch DNA had no effect on the fluorescence efficiency.