RAMAN AND HYPER RAYLEIGH SPECTROSCOPY OF NANOSCALE BIOLOGICAL INTERFACE

纳米级生物界面的拉曼和超瑞利光谱

• 批准号: 7336111
• 负责人: Paresh Chandra Ray
• 金额: $ 1.58万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336111
• 关键词: RAMAN; HYPER; RAYLEIGH; SPECTROSCOPY; NANOSCALE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have demonstrated for the first time that the hyper¿Rayleigh Scattering (HRS), which has emerged over the past decade as a powerful method to determine the microscopic non-linear optical (NLO) properties of species in solution, could achieve the ultra-sensitive detection of single base-pair mismatch in oligonucleotide strands without any covalent tagging of dyes. We have also reported 2 laser induced fluorescence (LIF) studies of ss-DNA hybridization based on fluorescence quenching by gold nanoparticles through a distant dependent process. We have shown that the fluorescence of ss-DNA increases by a factor of 100 when it binds to a complementary DNA while the addition of single-base mismatch DNA had no effect on the fluorescence efficiency.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们首次证明了超瑞利散射（HRS），这已经出现在过去的十年中作为一个强大的方法来确定微观非线性光学（NLO）性质的物种在溶液中，可以实现超灵敏检测单碱基对错配的寡核苷酸链没有任何共价标记的染料。我们还报道了两个激光诱导荧光（LIF）研究ss-DNA杂交的基础上荧光猝灭的金纳米粒子通过一个遥远的依赖过程。我们已经表明，当ss-DNA与互补DNA结合时，其荧光增加100倍，而添加单碱基错配DNA对荧光效率没有影响。