Discovery of cGAS Inhibitors for Interferon-Driven Autoimmune Diseases

发现用于治疗干扰素驱动的自身免疫性疾病的 cGAS 抑制剂

• 批准号: 10258171
• 负责人: Robert G Lowery
• 金额: $ 98.88万
• 依托单位: BELLBROOK LABS, LLC
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-12 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10258171
• 关键词: Active Sites; African American; Animal Model; Apoenzymes; Autoantibodies; Autoimmune; Autoimmune Diseases; Autoimmunity; B-Lymphocytes; Binding; Biochemical; Biological Assay; Biological Availability; Blood; Brain; Cause of Death; Cell Cycle; Cell Death; Cell Line; Cell Membrane Permeability; Cells; Cessation of life; Chronic; Clinical; Clinical Trials; Collaborations; Complex; Crystallization; Cutaneous; Cyclic Nucleotides; DNA; Data; Detection; Dinucleoside Phosphates; Drug Targeting; Enzymes; Exposure to; Gene Expression; Guanosine Triphosphate; Head; Heart; Hispanics; Human; Immune; Immune response; Infiltration; Inflammatory; Innate Immune Response; Interferon Type I; Interferon-alpha; Interferon-beta; Interferons; Investigation; Kidney; Knock-out; Lead; Ligands; Lung; Lupus; Medical; Modeling; Molecular; Molecular Conformation; Molecular Weight; Monitor; Mus; Nucleic Acids; Oral; Oral Administration; Organ; Pain; Pathology; Pathway interactions; Patients; Periodicity; Pharmaceutical Chemistry; Pharmaceutical Preparations; Phase; Phase I Clinical Trials; Photosensitivity; Physiological; Production; Property; Proteins; Reporting; Research; Rheumatoid Arthritis; Rheumatology; Safety; Second Messenger Systems; Ships; Signal Induction; Signal Transduction; Skin; Small Business Innovation Research Grant; Stimulator of Interferon Genes; Structure; Sunlight; Symptoms; Systemic Lupus Erythematosus; T-Cell Activation; Testing; Therapeutic; Therapeutic Intervention; Time; Tissues; UV induced; Universities; Washington; Woman; Work; animal efficacy; autocrine; belimumab; common symptom; curative treatments; cytokine; dimer; drug candidate; drug development; ds-DNA; efficacy evaluation; efficacy study; improved; in vivo; inhibitor/antagonist; innovation; lead optimization; microbial; monocyte; monomer; mortality; mouse model; paracrine; pathogenic microbe; premature; receptor; residence; response; scaffold; sensor; small molecule

Summary We are developing small molecule antagonists for cyclic GAMP synthase (cGAS) to identify a candidate drug molecule for lupus. Systemic lupus erythematosus (SLE), or simply lupus, is the second most common autoimmune disease next to rheumatoid arthritis; there are at least 300,000 patients in the U.S. alone and well over a million globally. The unmet medical need is enormous: lupus patients suffer from a 67% increase in mortality rate with damage to major organs in 50% of cases; e.g., heart, lung, kidneys, and brain; lupus was the 5th leading cause of death among young African American and Hispanic women in the U.S. from 2011-2015. There are no curative treatments for lupus, and only one drug (Benlysta) has been approved in the last 50 years. Lupus pathology is driven by type I interferons (IFNs), and the immune sensor, cyclic GAMP synthase (cGAS), is the trigger for type I IFN induction. DNA from dying cells binds to catalytically inactive cGAS to form an activated complex, triggering production of a unique cyclic nucleotide second messenger, cyclic GAMP (cGAMP). cGAMP binds to the STING protein to induce expression of type I IFNs, with autocrine and paracrine effects that lead to activation of T- and B-cells and auto-antibody production, precipitating a vicious cycle of cell death and autoimmunity. Using an innovative HTS assay developed under a separate SBIR, we discovered two promising cGAS antagonist chemotypes (40783 and 50101) that function via distinct mechanisms and have made substantial progress on increasing their biochemical and cellular potency while maintaining ADME properties predictive of good oral bioavailability. Our structural data indicate that the 40783 chemotype has allosteric binding properties and may stabilize an inactive cGAS conformation, properties which we will leverage in Phase II to develop a highly selective lead molecule with a long residence time. The 50101 chemotype appears to bind specifically to a hypersensitized cGAS-Mn-DNA complex, which could lead to an improved therapeutic window. In Phase II we propose to: 1) further optimize the potency, selectivity and ADME properties of the two chemotypes and 2) test their efficacy in an innovative model for UV-induced photosensitivity that replicates key aspects of SLE pathology and aligns closely with our clinical strategy. The animal efficacy studies will be performed in collaboration with Keith Elkon, Head of Rheumatology at University of Washington, Seattle, who developed the mouse photosensitivity model and has pioneered research on the involvement of the cGAS/STING pathway in lupus. Most investigational lupus drugs target the downstream effects of type I IFNs, a strategy that is akin to plugging holes in a sinking ship. The development of drugs that target cGAS, the upstream molecular trigger for nucleic-acid driven type I IFN production could revolutionize the treatment of lupus along with a growing list of cGAS- driven autoimmune and inflammatory conditions.

总结 我们正在开发环GAMP合成酶（cGAS）的小分子拮抗剂，以确定候选药物 狼疮的分子系统性红斑狼疮（SLE），或简称狼疮，是第二常见的 自身免疫性疾病仅次于类风湿性关节炎;仅在美国就有至少30万患者， 全球超过一百万。未得到满足的医疗需求是巨大的：狼疮患者的患病率增加了67%， 50%的病例中主要器官受损的死亡率;例如，心脏、肺、肾和大脑;狼疮是 2011年至2015年，美国年轻的非洲裔美国人和西班牙裔妇女的第五大死亡原因。 狼疮没有治愈性治疗方法，在过去的50年里只有一种药物（Benlysta）被批准。 狼疮病理是由I型干扰素（IFN）和免疫传感器环GAMP合酶驱动的 （cGAS）是I型IFN诱导的触发物。来自垂死细胞的DNA与无催化活性的cGAS结合， 一种激活的复合物，触发独特的环核苷酸第二信使环GAMP的产生 （cGAMP）。cGAMP与STING蛋白结合以诱导I型IFN的表达，具有自分泌和旁分泌作用。 导致T细胞和B细胞活化和自身抗体产生的作用，促使细胞恶性循环， 死亡和自身免疫 使用在单独SBIR下开发的创新HTS测定，我们发现了两种有前途的cGAS 拮抗剂化学型（40783和50101）通过不同的机制发挥作用，并取得了实质性进展 增加其生化和细胞效力，同时保持ADME特性，预测良好的口服给药效果。 生物利用度我们的结构数据表明，40783化学型具有变构结合特性， 稳定非活性cGAS构象，我们将在第二阶段利用这些性质来开发高度 具有长停留时间的选择性铅分子。50101化学型似乎特异性结合于 高致敏cGAS-Mn-DNA复合物，这可能导致改善的治疗窗口。在第二阶段，我们 建议：1）进一步优化两种化学型的效力、选择性和ADME性质，2）测试它们的 在UV诱导的光敏性的创新模型中的有效性，该模型复制了SLE病理学的关键方面， 这与我们的临床策略密切相关。动物有效性研究将与基思埃尔康合作进行， 西雅图的华盛顿大学的流变学负责人，他开发了小鼠光敏性模型， 开创了cGAS/STING通路参与狼疮的研究。 大多数研究性狼疮药物靶向I型干扰素的下游效应，这是一种类似于 把沉船上的洞堵上靶向cGAS的药物的开发，cGAS是癌症的上游分子触发剂。 核酸驱动的I型IFN产生可以彻底改变狼疮的治疗，沿着越来越多的cGAS- 导致自身免疫和炎症