Development of a handheld PEST-pen device for the rapid detection of organophosphate insecticides on food and clothing

开发手持式 PEST 笔装置,用于快速检测食品和衣物上的有机磷杀虫剂

基本信息

  • 批准号:
    10259769
  • 负责人:
  • 金额:
    $ 51.51万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-03-01 至 2024-08-31
  • 项目状态:
    已结题

项目摘要

ABSTRACT The neurotoxicity of organophosphate (OP) insecticides in humans may result from dermal or inhalation exposure on clothing and machinery or orally from the residue on food. In the latter context, the health consequences associated with the use of OP insecticides, especially in children, could be reduced by monitoring produce and eliminating the consumption of OP- contaminated food. PlantVax currently employs the Nicotiana benthamiana (N.b.) plant expression system to produce high levels of recombinant human acetylcholinesterase (rHuAChE) which is used in devices e.g. the FLIR CAD-pen, for the detection of trace amounts of OP nerve agents for military purposes. In this proposal, PlantVax aims to develop for food safety purposes, a simplified, hand-held, portable, self-contained and inexpensive PEST-pen to rapidly detect phosphorothionate insecticides on food, clothing and machinery. However unlike active OP nerve agents, OP insecticides exhibit a wide range of bimolecular rate constants (ki) ranging from ki = 101M-1min-1 to 109M-1min-1) against esterases e.g AChE. These fall into two categories (i) those with high ki (>104M-1min-1) which contain an “oxon-like” bond and can be used immediately to produce a functional PEST-pen in Phase II and (ii) those with low ki (~101-3M-1min-1) which have a P=S bond and will need to be converted by oxidative desulfuration into the active oxon form by cytochrome P450 and NADPH for their toxicity and detection. The new PEST-pen device will utilize the same esterase-based embedded chemistry as the CAD-pen with a yellow to red color change as a read-out, but with two important modifications required to increase the ki and thus the color change (i) the use of recombinant human CES (rHuCES) also produced in N.b. plants immobilized into the pen sponge as the target enzyme since Phase I results indicated a 10-fold to 1,000-fold increase in ki against both thion and oxon forms with this enzyme and (ii) the integration of P450 and NADPH into the pen to convert kilow to kihigh to enable the detection of many highly toxic OP insecticides which might have otherwise gone undetected. In Phase II, the chemistry will be optimized in twenty functional printed pens and then two thousand beta- prototype PEST-pens, which are compatible with the chemistry, will be designed and produced by injection molding for testing in the field. It is planned to initially test vegetable and fruit samples in markets and farms in the USA, Asia and Central America to demonstrate the utility of the PEST- pen globally.
摘要 有机磷(OP)杀虫剂对人体的神经毒性可通过皮肤或 吸入暴露于衣服和机器或口服暴露于食物残留物。在后一 在这方面,与使用OP杀虫剂有关的健康后果,特别是在 儿童,可以减少监测生产和消除消费的OP- 被污染的食物PlantVax目前雇用本氏烟草(Nicotiana benthamiana(N.b.)植物 生产高水平重组人乙酰胆碱酯酶(rHuAChE)的表达系统 用于FLIR CAD笔等设备中,用于检测痕量OP神经 用于军事目的。在这项提案中,PlantVax旨在为食品安全目的开发, 一种简单的、手持式、便携式、独立的和廉价的PEST笔, 硫代磷酸酯杀虫剂在食品、衣物和机械上的应用。然而,与活动OP神经不同, OP杀虫剂表现出宽范围的双分子速率常数(ki),范围为ki = 101 M-1 min-1至109 M-1 min-1)对酯酶如AChE的抑制作用。这分为两类:(一) 具有高Ki(> 104 M-1 min-1),含有“oxon样”键,可立即用于 在阶段II中产生功能性PEST-笔和(ii)具有低ki(~101- 3 M-1 min-1)的那些,其具有 P=S键,需要通过氧化脱硫转化为活性氧磷形式, 细胞色素P450和NADPH的毒性和检测。新的PEST笔设备将 使用与CAD笔相同的基于酯酶的嵌入式化学物质,颜色为黄色至红色 改变为读出,但需要两个重要的修改,以增加ki, 颜色变化(i)使用也在N.b.中产生的重组人CES(rHuCES)。植物 固定到笔海绵中作为靶酶,因为I期结果表明, 1,000倍增加ki对硫和oxon形式与这种酶和(ii) 将P450和NADPH整合到笔中,将kilow转换为kihigh,以检测 许多高毒性有机磷杀虫剂,否则可能会被发现。在第二阶段, 化学将在20支功能性打印笔中进行优化,然后是2000支beta- 与化学相容的原型PEST笔将由以下公司设计和生产: 用于现场测试的注塑成型。计划在2004年对蔬菜和水果样品进行初步检测, 市场和农场在美国,亚洲和中美洲,以证明实用的PEST- 笔全球。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Yvonne J Rosenberg其他文献

Yvonne J Rosenberg的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Yvonne J Rosenberg', 18)}}的其他基金

Zwitterionic oxime (RS194B) to reverse advanced symptoms and lethality of organophosphate pesticide exposure
两性离子肟 (RS194B) 可逆转有机磷农药暴露的晚期症状和致死率
  • 批准号:
    9621314
  • 财政年份:
    2018
  • 资助金额:
    $ 51.51万
  • 项目类别:
Development of a handheld PEST-pen device for the rapid detection of organophosphate insecticides on food and clothing
开发手持式 PEST 笔装置,用于快速检测食品和衣物上的有机磷杀虫剂
  • 批准号:
    10079784
  • 财政年份:
    2018
  • 资助金额:
    $ 51.51万
  • 项目类别:
Plant-derived HIV neutralizing mAbs for passive immunotherapy in newborn macaques
用于新生猕猴被动免疫治疗的植物源性 HIV 中和单克隆抗体
  • 批准号:
    9312216
  • 财政年份:
    2016
  • 资助金额:
    $ 51.51万
  • 项目类别:
Efficacy and Safety of an Aerosolized Recombinant Butyrylcholinesterase Pretreatm
雾化重组丁酰胆碱酯酶预处理的功效和安全性
  • 批准号:
    8738719
  • 财政年份:
    2009
  • 资助金额:
    $ 51.51万
  • 项目类别:
Aerosol Delivery of a Recombinant Butyrylcholinesterase "BioShield" to Protect Ag
气溶胶递送重组丁酰胆碱酯酶“BioShield”以保护 Ag
  • 批准号:
    7612606
  • 财政年份:
    2009
  • 资助金额:
    $ 51.51万
  • 项目类别:
Passive immunotherapy using plant-derived broadly HIV-1 neutralizing MAbs to prev
使用植物来源的广泛 HIV-1 中和单克隆抗体进行被动免疫疗法来预防
  • 批准号:
    8210828
  • 财政年份:
    2009
  • 资助金额:
    $ 51.51万
  • 项目类别:
Efficacy and Safety of an Aerosolized Recombinant Butyrylcholinesterase Pretreatm
雾化重组丁酰胆碱酯酶预处理的功效和安全性
  • 批准号:
    8523566
  • 财政年份:
    2009
  • 资助金额:
    $ 51.51万
  • 项目类别:
Aerosol Delivery of a Recombinant Butyrylcholinesterase "BioShield" to Protect Ag
气溶胶递送重组丁酰胆碱酯酶“BioShield”以保护 Ag
  • 批准号:
    7888255
  • 财政年份:
    2009
  • 资助金额:
    $ 51.51万
  • 项目类别:
Efficacy and Safety of an Aerosolized Recombinant Butyrylcholinesterase Pretreatm
雾化重组丁酰胆碱酯酶预处理的功效和安全性
  • 批准号:
    9121644
  • 财政年份:
    2009
  • 资助金额:
    $ 51.51万
  • 项目类别:
Passive immunotherapy using plant-derived broadly HIV-1 neutralizing MAbs to prev
使用植物来源的广泛 HIV-1 中和单克隆抗体进行被动免疫疗法来预防
  • 批准号:
    7685238
  • 财政年份:
    2009
  • 资助金额:
    $ 51.51万
  • 项目类别:

相似海外基金

Effect of Acetylcholinesterase inhibitors on Bone Metabolism and Fracture Risk Factors among older adults with mild to moderate Alzheimer's Disease
乙酰胆碱酯酶抑制剂对患有轻至中度阿尔茨海默病的老年人骨代谢和骨折危险因素的影响
  • 批准号:
    10739853
  • 财政年份:
    2023
  • 资助金额:
    $ 51.51万
  • 项目类别:
Acetylcholinesterase Complex Protein-Protein Interactions as Drug Targets Against Organophosphate-induced Neurotoxicity.
乙酰胆碱酯酶复合物蛋白质-蛋白质相互作用作为抗有机磷诱导的神经毒性的药物靶点。
  • 批准号:
    10772738
  • 财政年份:
    2023
  • 资助金额:
    $ 51.51万
  • 项目类别:
Acetylcholinesterase Complex Protein-Protein Interactions as Drug Targets Against Organophosphate-induced Neurotoxicity.
乙酰胆碱酯酶复合物蛋白质-蛋白质相互作用作为抗有机磷诱导的神经毒性的药物靶点。
  • 批准号:
    10303546
  • 财政年份:
    2021
  • 资助金额:
    $ 51.51万
  • 项目类别:
Machine learning approaches to predict Acetylcholinesterase inhibition
预测乙酰胆碱酯酶抑制的机器学习方法
  • 批准号:
    10378934
  • 财政年份:
    2021
  • 资助金额:
    $ 51.51万
  • 项目类别:
Investigating the in vivo targets and mechanism of action of a toxic acetylcholinesterase-derived peptide upregulated in Alzheimer's disease
研究阿尔茨海默病中上调的有毒乙酰胆碱酯酶衍生肽的体内靶点和作用机制
  • 批准号:
    2480200
  • 财政年份:
    2020
  • 资助金额:
    $ 51.51万
  • 项目类别:
    Studentship
Improved Therapeutics for the Resurrection of the Aged Form of Acetylcholinesterase
老化乙酰胆碱酯酶复活的改进疗法
  • 批准号:
    9977281
  • 财政年份:
    2019
  • 资助金额:
    $ 51.51万
  • 项目类别:
Improved Therapeutics for the Resurrection of the Aged Form of Acetylcholinesterase
老化乙酰胆碱酯酶复活的改进疗法
  • 批准号:
    10238898
  • 财政年份:
    2019
  • 资助金额:
    $ 51.51万
  • 项目类别:
Elucidation of the cell protective mechanism of acetylcholinesterase variants in cardiomyocyte and approach to therapy for myocardial infarction
心肌细胞乙酰胆碱酯酶变异体的细胞保护机制的阐明及心肌梗死的治疗方法
  • 批准号:
    18K15850
  • 财政年份:
    2018
  • 资助金额:
    $ 51.51万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Effects of Alzheimer's disease drugs inhibiting acetylcholinesterase on prion replication.
阿尔茨海默病药物抑制乙酰胆碱酯酶对朊病毒复制的影响。
  • 批准号:
    481759-2015
  • 财政年份:
    2015
  • 资助金额:
    $ 51.51万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Reactivation of Aged Acetylcholinesterase: Design and Development of Novel Therap
老化乙酰胆碱酯酶的重新激活:新型疗法的设计和开发
  • 批准号:
    8735550
  • 财政年份:
    2014
  • 资助金额:
    $ 51.51万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了