Metabolomics Core

代谢组学核心

• 批准号: 10262784
• 负责人: Daniel Crooks
• 金额: $ 110.91万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10262784
• 关键词: Bacteria; Biochemical; Biological; Biological Markers; Biopsy; Blood; Cancer Detection; Cancer Model; Carcinogenesis Mechanism; Cells; Chemicals; Chills; Clinical; Coiled Bodies; Core Facility; Custom; Data Collection; Detection; Development; Dose; Electrospray Ionization; Equipment; Future; Goals; Housing; Human; Immune system; Infusion procedures; Ions; Isotopes; Laboratories; Lead; Magnetic Resonance Spectroscopy; Mammals; Mass Spectrum Analysis; Metabolic; Metabolism; Methodology; Methods; Monitor; NMR Spectroscopy; Nuclear; Operative Surgical Procedures; Pathway interactions; Patients; Plants; Preparation; Procedures; Pyruvate; Resolution; Sampling; Specimen; Therapeutic Intervention; Time; Tissue Sample; Tissues; Toxic effect; Tracer; Tumor-Derived; United States National Institutes of Health; Urine; Validation; base; cancer biomarkers; cancer diagnosis; cancer prevention; cancer recurrence; cancer therapy; clinical center; instrumentation; mass spectrometer; metabolic imaging; metabolomics; method development; new therapeutic target; novel; novel strategies; pre-clinical; small molecule; spectroscopic imaging; stable isotope; tumor; tumor metabolism; ultra high resolution

We have undertaken the development of facilities within Building 10 for the conduct of metabolomics studies and metabolic imaging with a focus on isotope-resolved analysis of clinical samples and patient-based studies. The Mass Spectrometry and Sample Preparation Core Facility is geared toward targeted, ultra-high resolution stable isotope-resolved metabolomics as well as careful clinical tissue sample extraction and preparation methodologies. Renovation of the laboratory spaces comprising the Mass Spectrometry and Sample Preparation Core Facility will be completed in FY20, and acquisition of analytical equipment and instrumentation is currently underway. The first mass spectrometer to be installed will be a 1,000,000 resolving power Thermo Orbitrap Fusion Lumos spectrometer equipped with a Dionex IC-6000 Ion Chromatograph and an Advion TriVersa Nanomate direct infusion electrospray ionization modules, which will be used to develop workflows tailored for targeted quantitation of approximately 120 cellular metabolites. Renovation of the space housing the Clinical NMR Metabolomics Facility was completed in November of FY20, and delivery and installation of a 700MHz Bruker NMR magnet, triple inverse resonance cryoprobe and high-capacity chilled autosampler was completed in May, 2020. Data collection and method development for NMR analysis of metabolite extracts from clinical tissue specimens obtained during surgical and biopsy procedures in the NIH Clinical Center are currently underway. Finally, procurement of a custom 13C body coil and consumables for validation and preparation of 13C1-pyruvate doses for future clinical 13C-hyperpolarized metabolic imaging are taking place.

我们已经开发了建筑物10中的设施，以进行代谢组学研究和代谢成像，重点是对临床样本和基于患者的研究的同位素分解分析。质谱和样品制备核心设施旨在实现靶向超高分辨率稳定的同位素分辨代谢组，以及仔细的临床组织样品提取和制备方法。包括质谱和样品制备核心设施的实验室空间的翻新工程将在200财年完成，目前正在收购分析设备和仪器。第一个要安装的质谱仪将是配备Dionex IC-6000离子色谱仪和Adriversa Nanomate Direct Infusion Extrospray电源电离模块的1,000,000个解决功率热Orbitrap Fusion Lumos光谱仪，该模块将用于开发用于针对目标定量的近似于120个细胞的定量工作表。临床NMR代谢组学设施的临床空间的翻新工程于11月20日完成，以及700MHz Bruker NMR磁铁的交付和安装，三重逆共振冷冻螺旋桨和高能力冰冷的冰冷的自动采样器在2020年的临床和方法中均进行了验证。 NIH临床中心的活检程序目前正在进行中。最后，正在进行定制的13C身体线圈和消耗品，以验证和制备13C1-丙酮酸剂量，以进行未来的临床13C-HYPERPARLALIZERIZER化代谢成像。