CORE C- Protein Expression and Purification

CORE C-蛋白表达和纯化

• 批准号: 10268696
• 负责人: Yunmei Wang
• 金额: $ 31.88万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10268696
• 关键词: Adopted; Animal Model; Animals; Archives; Atherosclerosis; Blood; Blood Vessels; Cardiovascular Models; Cell Adhesion; Cells; Clinical; Disease model; Doctor of Philosophy; Ensure; Equipment; Goals; Heart Hypertrophy; Individual; Institutional Review Boards; Laboratories; Liquid substance; Lupus Erythematosus; Modeling; Mus; Pristane; Publications; Publishing; Research; Research Personnel; Research Support; Sampling; Services; Signal Transduction; Specimen; Stroke; Technical Expertise; Techniques; Thrombosis; Tissues; Training; University Hospitals; Urine; Variant; Vasculitis; Venous Thrombosis; animal resource; animal tissue; cost effective; experience; in vivo; innovation; mouse model; protein expression; protein purification; skills; success; vascular injury

Project Summary/Abstract The proposed Animal Models and Tissue Analysis Core (Core C) is an essential component to the goals of this PPG. To support the research strategies and success of the individual projects proposed in this PPG application, Core C will serve each of the 3 projects of the PPG with a variety of animal models of arterial thrombosis, atherosclerosis, venous thrombosis, and spontaneous and pristane-induced systematic lupus erythematosus (SLE). In addition, it will provide comprehensive tissue, blood and urine sample analysis for each of the 3 projects. Furthermore, Core C will train fellows and technical staff of the individual projects to perform animal studies. Lastly, Core C has access to archival FFPE specimens at the University Hospitals that is overseen by Dr. Gilmore, a Co-Investigator of Core C, who will ensure that all IRB requirements for discarded tissue research have been met. Dr. Gilmore will locate appropriate tissues for the research of the PPG and provide Core C with the necessary clinical information to facilitate the analyses. The services Core C will provide require specialized technical skills and equipment. Core C will alleviate the need for each of the investigators to establish the techniques in their individual laboratories, thereby increasing efficiency, reducing experimental variation, and providing economy of scale. The main advantages and innovation of Core C are to provide a dedicated, highly skillful, accessible and cost effective resource for animal models and tissue analysis. The proposed Core C has extensive experience in animal models for cardiovascular research focusing on various disease models, including thrombosis, atherosclerosis, vasculitis, stroke, cardiac hypertrophy, analysis and quantitation of mouse tissue and fluids specimens. The models and analysis have been well-established and published (see Biosketch of Core C leader). Building on the experience with animal models and tissue analysis, Core C has also established both spontaneous and pristane-induced SLE models (See Figures 6 and 7 in Research Plan). Core C will be led by Yunmei Wang PhD, who has extensive experience in murine models of vascular injury, thrombosis, atherosclerosis, stroke, and cardiac hypertrophy. In addition, Dr. Wang's over 25-years' research experience working with animals has prepared her well for any additional expertise and skills involving animals if needed for Core C. Core C will quickly adopt any innovative animal models once they become available and are determined appropriate for the purpose of the projects. Furthermore, Dr. Wang has collaborated with all project leaders of the PPG (see publication in Biosketch).

项目概要/摘要 拟议的动物模型和组织分析核心（核心 C）是一个重要组成部分 实现本 PPG 的目标。支持个人的研究策略和成功 在此 PPG 申请中提出的项目中，Core C 将服务于 PPG 的 3 个项目中的每一个 建立动脉血栓、动脉粥样硬化、静脉血栓等多种动物模型 自发性和降植烷诱发的系统性红斑狼疮（SLE）。此外，还将 为这 3 个项目中的每一个项目提供全面的组织、血液和尿液样本分析。 此外，Core C 将培训各个项目的研究员和技术人员来执行 动物研究。最后，核心 C 可以访问大学的档案 FFPE 样本 由 Core C 联合研究员 Gilmore 博士监督的医院，他将确保所有医院 已满足 IRB 对废弃组织研究的要求。吉尔摩博士将找到 用于 PPG 研究的合适组织，并为 Core C 提供必要的临床 信息以促进分析。 Core C 将提供的服务需要专门的 技术能力和设备。核心 C 将减轻每个研究人员的需要 在各自的实验室建立技术，从而提高效率，减少 实验变化，并提供规模经济。主要优点及创新点 核心 C 是为以下人员提供专用、高技能、可访问且具有成本效益的资源： 动物模型和组织分析。 拟议的核心C在心血管动物模型方面拥有丰富的经验 研究重点是各种疾病模型，包括血栓形成、动脉粥样硬化、血管炎、 中风、心脏肥大、小鼠组织和体液样本的分析和定量。 模型和分析已经完善并发表（参见 Core C 的 Biosketch） 领导者）。基于动物模型和组织分析的经验，Core C 还 建立了自发性和降植烷诱导的 SLE 模型（见图 6 和 7） 研究计划）。 Core C将由王云梅博士领导，她在以下方面拥有丰富的经验 血管损伤、血栓形成、动脉粥样硬化、中风和心脏肥大的小鼠模型。 此外，王博士超过25年的动物研究经验为 如果核心 C 需要，她很乐意提供涉及动物的任何其他专业知识和技能。 核心 C 一旦可用并确定，将迅速采用任何创新的动物模型 适合项目的目的。此外，王博士还与所有 PPG 的项目负责人（参见 Biosketch 中的出版物）。