CORE C- Protein Expression and Purification

项目摘要/摘要 建议的动物模型和组织分析核心(核心C)是必不可少的组成部分 这个PPG的目标。支持个人的研究策略和成功 此PPG申请中提出的项目，核心C将为PPG的3个项目中的每个项目提供服务 动脉血栓形成、动脉粥样硬化、静脉血栓形成的各种动物模型 自发性和泼尼松诱导的系统性红斑狼疮(SLE)此外，它还将 为三个项目的每个项目提供全面的组织、血液和尿液样本分析。 此外，核心C将培训个别项目的研究员和技术人员以执行 动物研究。最后，核心C可以访问大学的FFPE档案样本 医院由吉尔莫博士监督，他是核心C的联合调查员，他将确保所有 IRB对废弃组织研究的要求已经达到。吉尔莫博士会找到 为PPG研究提供合适的组织，并为Core C提供必要的临床 为分析提供便利的信息。Core C将提供所需的专门服务 技术技能和设备。核心C将减轻每个调查人员需要 在各自的实验室建立技术，从而提高效率，减少 试验性变化，并提供规模经济。的主要优势和创新之处 核心C是为以下方面提供专门的、高度熟练的、可访问的且经济高效的资源 动物模型和组织分析。 建议的Core C在心血管动物模型方面拥有丰富的经验 专注于各种疾病模型的研究，包括血栓形成、动脉粥样硬化、血管炎、 中风、心肌肥大、小鼠组织和体液标本的分析和定量。 模型和分析已经很好地建立并发表了(见C核心的生物示意图 领袖)。在动物模型和组织分析经验的基础上，Core C还 建立了自发性和Pristane诱导的SLE模型(见图6和图7 研究计划)。核心C将由王云梅博士领导，他在以下领域拥有丰富的经验 血管损伤、血栓形成、动脉粥样硬化、中风和心肌肥厚的小鼠模型。 此外，王博士超过25年的动物研究经验也为 如果Core C需要任何与动物有关的其他专业知识和技能，请与她联系。 一旦任何创新的动物模型可用并确定下来，就会迅速采用它们 适用于项目的目的。此外，王博士还与所有 PPG的项目负责人(见Biosketch中的出版物)。

Project Summary/Abstract The proposed Animal Models and Tissue Analysis Core (Core C) is an essential component to the goals of this PPG. To support the research strategies and success of the individual projects proposed in this PPG application, Core C will serve each of the 3 projects of the PPG with a variety of animal models of arterial thrombosis, atherosclerosis, venous thrombosis, and spontaneous and pristane-induced systematic lupus erythematosus (SLE). In addition, it will provide comprehensive tissue, blood and urine sample analysis for each of the 3 projects. Furthermore, Core C will train fellows and technical staff of the individual projects to perform animal studies. Lastly, Core C has access to archival FFPE specimens at the University Hospitals that is overseen by Dr. Gilmore, a Co-Investigator of Core C, who will ensure that all IRB requirements for discarded tissue research have been met. Dr. Gilmore will locate appropriate tissues for the research of the PPG and provide Core C with the necessary clinical information to facilitate the analyses. The services Core C will provide require specialized technical skills and equipment. Core C will alleviate the need for each of the investigators to establish the techniques in their individual laboratories, thereby increasing efficiency, reducing experimental variation, and providing economy of scale. The main advantages and innovation of Core C are to provide a dedicated, highly skillful, accessible and cost effective resource for animal models and tissue analysis. The proposed Core C has extensive experience in animal models for cardiovascular research focusing on various disease models, including thrombosis, atherosclerosis, vasculitis, stroke, cardiac hypertrophy, analysis and quantitation of mouse tissue and fluids specimens. The models and analysis have been well-established and published (see Biosketch of Core C leader). Building on the experience with animal models and tissue analysis, Core C has also established both spontaneous and pristane-induced SLE models (See Figures 6 and 7 in Research Plan). Core C will be led by Yunmei Wang PhD, who has extensive experience in murine models of vascular injury, thrombosis, atherosclerosis, stroke, and cardiac hypertrophy. In addition, Dr. Wang's over 25-years' research experience working with animals has prepared her well for any additional expertise and skills involving animals if needed for Core C. Core C will quickly adopt any innovative animal models once they become available and are determined appropriate for the purpose of the projects. Furthermore, Dr. Wang has collaborated with all project leaders of the PPG (see publication in Biosketch).