CORE B- Animal Models and Tissue Analysis

项目摘要 该计划项目的主要目标是了解生理学 整合素的病理生理功能在血液和血管细胞中受到调节。“蛋白质表达” 核心B将帮助三个项目中的每一个通过提供蛋白质来实现这一共同目标 而cDNA需要分析这些分子机制。该计划项目所需的蛋白质 是整合素、talin、kindlins、IRAK1、paxlin以及这些蛋白质的片段。所需的cDNA是 表达整合素、talin、paxlin、kindlins和IRAK1，这些蛋白的突变形式和片段。 通过提供具有良好特性的蛋白质和cDNA，这个核心产生了对所有人都有用的可靠试剂 该计划的三个项目。处理各项目所建议的结构与职能的关系 该计划需要大量的野生型和突变型的目标蛋白。两种细菌 并且需要在哺乳动物中表达蛋白质。蛋白质表达的集中化与诱变 避免在各个实验室内重复不必要的程序，提供对符合以下条件的设备的访问 蛋白质表达和纯化所需的，并允许这些任务有效地执行和 经济上由经验丰富的人员负责。核心B也有一个培训要素。核心人员将培训 个别项目中的研究员和技术人员，以执行蛋白质纯化，细菌和哺乳动物 表达、诱变策略和蛋白质表征方法。

Project Summary The primary goal of this Program Project is to understand the molecular mechanisms by which the physiological and pathophysiological functions of integrins are regulated in blood and vascular cells. The “Protein Expression and Purification” Core B will assist each of the three projects realize this common goal by providing the proteins and cDNAs needed to analyze these molecular mechanisms. The proteins needed by the projects of the program are integrins, talin, kindlins, IRAK1, paxillin, and fragments of these proteins. The cDNAs needed are for expression of the integrins, talin, paxillin, kindlins, and IRAK1, mutant forms of and fragments of these proteins. By providing well-characterized proteins and cDNAs, this core creates reliable reagents that are useful to all three Projects of the Program. To address the structure-function relationships proposed in each project of the program requires substantial amounts of the wild-type and mutant forms of the target proteins. Both bacterial and mammalian expression of proteins is needed. The centralization of protein expression and mutagenesis avoids unneeded replication of procedures within individual laboratories, provides access to equipment that is needed for protein expression and purification, and allows these tasks to be performed efficiently and economically by experienced personnel. There is also a training element to Core B. The Core personnel will train fellows and technical staff within the individual projects to perform protein purification, bacterial and mammalian expression, mutagenesis strategies and in protein characterization approaches.