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Core C: Human Thymus Core

核心C：人类胸腺核心

基本信息

批准号：
10251296
负责人：
Laura P. Hale
金额：
$ 36.59万
依托单位：
UNIVERSITY OF TX MD ANDERSON CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10251296
关键词：
Address Adolescent Adult Age Antigen-Presenting Cells Architecture Bioinformatics Biology Biostatistics Core Cell Compartmentation Cell Separation Cells Characteristics Collection Communities Complement Consultations Critical Pathways Data Databases Doctor of Philosophy Ensure Flow Cytometry Foundations Frozen Sections Gene Expression Gene Expression Profile Generations Goals Growth Hematopoietic Heterogeneity Histology Homeostasis Human Immunophenotyping Institutes Institution Knowledge Longevity Mediating Modernization Molecular Molecular Analysis Monitor Mus Newborn Infant Pathologist Pathology Pathway interactions Perinatal Phenotype Population Population Analysis Procedures Production Quality Control Records Research Research Personnel Research Project Grants Research Support Resource Sharing Resources Scheme Self Tolerance Services Signal Pathway Specific qualifier value Standardization Stromal Cells T-Cell Receptor Genes T-Lymphocyte Thinking Thymic Tissue Thymic epithelial cell Thymus Gland Translations Vaccines Work base biobank cell preparation effector T cell gene expression database human tissue innovation molecular phenotype mouse model pathogen phenotypic data programs service utilization synergism transcriptome sequencing

项目摘要

Abstract The Research Projects (RP) of this comprehensive discovery-based P01 seek to identify specific changes in the transcriptional profiles and signaling pathways that control thymic epithelial cell (TEC), stromal cell, and thymic hematopoietic antigen presenting cell (HAPC) compartment dynamics and lineage hierarchies during the transition from perinatal thymus expansion to juvenile/adult homeostasis. Studies in the RPs will focus on murine models, but the questions they seek to address require comparison of mouse and human thymus biology across the perinatal to juvenile transition. However, human thymus tissue is not readily available at most institutions and cross-species comparisons are not always straight-forward. In addition, the emphasis of this P01 on deciphering heterogeneity in mostly non-overlapping cellular subsets of TECs (RP1, RP2), other stromal cells, (RP2), and HAPCs (RP3) mean that data relevant to all three RPs can potentially be obtained from each human tissue studied. Integration of this centralized Human Thymus Core into the P01 has resolved these issues. Core C services will allow RP 1-3 to address the knowledge gaps regarding translation of their mouse data and previously unstudied aspects of human thymus biology, via three focused specific aims/Service Tasks: 1) Procurement and processing of human thymus tissues from healthy donors to meet project-specific translation needs; 2) Enrichment and immunophenotyping of TECs, other stromal cells, and HAPCs to validate human equivalent markers analogous to those identified in mice in RP 1-3; and 3) Molecular analysis of thymic stromal cells and HAPC subsets through generation of standardized, quality- controlled single cell (sc) and bulk RNA-seq data from sorted human thymus populations for analysis by Core B. In addition to providing critical services to the RPs, the comprehensive database of gene expression in subsets of TEC, stromal cells, and HAPCs from human thymus that will be generated by this Core will be a significant new resource for both human and mouse thymus research communities.

摘要