Human Target Verification and Thymic Function Core

核心C-摘要 人类目标验证和胸腺功能核心（核心C）被整合到该计划中， 在人胸腺、淋巴结（LN）和T细胞老化中提供靶点的概念验证， 通过创新方法和研究项目的小鼠模型确定的途径。的资产 核心C带来的程序包括核心领导人谁是世界公认的专业知识 人类胸腺生物学和衰老方面的专家以及杜克人类疫苗研究所（DHVI）人类胸腺 Biobank包含来自890多名1天至78岁的健康人类供体的组织和分离细胞， 正在收集新的标本。核心C还提供全面的组织学，专家委员会认证 病理学支持、小鼠和人sjTREC分析和胸腺基因表达谱分析。在未来 在该计划的五年中，核心C将继续加速对项目确定的概念验证。 人胸腺、LN和T细胞老化中的小鼠治疗靶点/途径，并独立监测胸腺 输出，通过两个重点服务任务或核心目标：1）定义、表征和提供人体组织 由指定年龄和性别的捐赠者组成的小组，以满足特定项目的翻译验证需求;以及2） 提供人类胸腺的中心组织学和流式细胞术分析，并从分子水平获得数据。 人和小鼠胸腺组织的分析。预计在续订期内使用核心C服务的情况如下： 这三个项目。向项目1-3提供核心C服务是整个项目协同的关键组成部分， 非常重要的是，将提供关键的概念验证，验证小鼠靶点/途径的适用性 人类胸腺的衰老这将有助于实现振兴战略的项目目标，以改善年龄- 相关的免疫缺陷和提高免疫防御感染和癌症作为个人的年龄。

CORE C- Abstract The Human Target Verification and Thymic Function Core (Core C) was integrated into this program to provide proof-of-concept verification, in human thymus, lymph node (LN), and T cell aging, of the targets and pathways identified by the innovative approaches and mouse models of the research projects. Assets that Core C brings to the program include the expertise of Core leaders who are widely acknowledged as world experts in human thymus biology and aging and the Duke Human Vaccine Institute (DHVI) Human Thymus Biobank that contains tissues and isolated cells from 890+ healthy human donors aged 1 day to 78 years, with ongoing collection of new specimens. Core C also provides comprehensive histology, expert board-certified pathology support, mouse and human sjTREC analysis and thymus gene expression profiling. Over the next five years of the Program, Core C will continue to accelerate proof-of-concept verification of project-identified mouse therapeutic targets/pathways in human thymus, LN, and T cell aging and independently monitor thymic output, through two focused Service Tasks or Core Aims: 1) To define, characterize, and supply human tissue panels from donors of defined age and sex to meet project-specific translation verification needs; and 2) To provide central histologic and flow cytometric analyses on human thymus and obtain data from molecular analyses on human and mouse thymus tissues. Use of Core C services in the renewal period is anticipated by all three projects. Delivery of Core C services to Projects 1-3 is a key component of overall program synergy, and very importantly, will provide critical proof-of-concept verification of applicability of mouse targets/pathways to aging in human thymus. This will facilitate project goals for rejuvenation strategies to ameliorate age- associated immune deficiencies and improve immune defense against infection and cancer as individuals age.