Modulation of neutrophil function through emperipolesis

通过伸入调节中性粒细胞功能

基本信息

  • 批准号:
    10091401
  • 负责人:
  • 金额:
    $ 17.59万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-02-01 至 2021-06-30
  • 项目状态:
    已结题

项目摘要

Project Summary For almost 50 years it has been known that neutrophils regularly appear within megakaryocytes (MKs), a histological phenomenon termed emperipolesis. Using new tools we developed to study this process in vitro and in vivo, we found that emperipolesis is common and increases with experimental inflammatory stress in a murine system. Neutrophils penetrate into the MK cytoplasm, donating a fraction of their surface membrane to platelets before emerging viable and intact. Our preliminary data show that neutrophils take up MK-derived exosomes during emperipolesis and emerge with enhanced migratory capacity. Building on these results, we explore the hypothesis that emperipolesis is a novel cell-in-cell interaction that modulates the function of neutrophils. We test this hypothesis in two independent but complementary aims. In Aim I, we examine the effect of emperipolesis, and in particular of MK exosomes, on neutrophil effector functions, metabolism, and in vivo migration in neutrophil-dependent K/BxN serum transfer arthritis. In Aim II, we employ whole-mount 3D marrow imaging, 2-photon in vivo microscopy, and specific MK deletion and potentially emperipolesis blockade to test the possibility that emperipolesis represents a quantitatively important pathway of neutrophil egress from bone marrow in vivo, including in experimental arthritis. Together, these studies will define a new form of cooperation between MKs and neutrophils that modulates the ability of neutrophils to participate in health and disease. More generally, building upon our recent identification of MKs as a source of IL-1 in arthritis, these studies continue to develop the understanding of MKs and neutrophils as participants in systemic inflammatory disease. These “high risk, high reward” studies of a previously overlooked biological process, widely conserved across mammalian species, will set the stage for an extended investigation of emperipolesis as a novel cell-in-cell phenomenon.
项目总结

项目成果

期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Peter A Nigrovic其他文献

Development of consensus best treatment plans for new-onset systemic juvenile idiopathic arthritis
  • DOI:
    10.1186/1546-0096-10-s1-a50
  • 发表时间:
    2012-07-13
  • 期刊:
  • 影响因子:
    2.300
  • 作者:
    Esi M Morgan DeWitt;Timothy Beukelman;Peter A Nigrovic;Karen Onel;Sampath Prahalad;Rayfel Schneider;Matthew Stoll;Carol A Wallace;Yukiko Kimura
  • 通讯作者:
    Yukiko Kimura
Disease characteristics and medication use in a multicenter cohort of children with juvenile idiopathic arthritis (JIA): preliminary analyses from the CARRAnet registry
  • DOI:
    10.1186/1546-0096-10-s1-a46
  • 发表时间:
    2012-07-13
  • 期刊:
  • 影响因子:
    2.300
  • 作者:
    Sarah Ringold;Timothy Beukelman;Esi M Morgan DeWitt;Marc Natter;Peter A Nigrovic;Yukiko Kimura
  • 通讯作者:
    Yukiko Kimura
Juvenile idiopathic arthritis is associated with potentially pathogenic glycosylation of IgG
  • DOI:
    10.1186/1546-0096-10-s1-a5
  • 发表时间:
    2012-07-13
  • 期刊:
  • 影响因子:
    2.300
  • 作者:
    Altan Ercan;Melissa Hazen;Mary Beth Son;Susan Kim;Fatma Dedeoglu;Robert P Sundel;Robert C Fuhlbrigge;Jing Cui;Nancy A Shadick;Michael E Weinblatt;Michael Spigarelli;David N Glass;Susan D Thompson;Peter A Nigrovic
  • 通讯作者:
    Peter A Nigrovic

Peter A Nigrovic的其他文献

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{{ truncateString('Peter A Nigrovic', 18)}}的其他基金

Impact of emperipolesis on platelet function
伸入对血小板功能的影响
  • 批准号:
    10705905
  • 财政年份:
    2022
  • 资助金额:
    $ 17.59万
  • 项目类别:
Modulation of neutrophil function through emperipolesis
通过伸入调节中性粒细胞功能
  • 批准号:
    10656013
  • 财政年份:
    2020
  • 资助金额:
    $ 17.59万
  • 项目类别:
T resident memory cells in arthritis
关节炎中的 T 常驻记忆细胞
  • 批准号:
    10179324
  • 财政年份:
    2019
  • 资助金额:
    $ 17.59万
  • 项目类别:
T resident memory cells in arthritis
关节炎中的 T 常驻记忆细胞
  • 批准号:
    10609770
  • 财政年份:
    2019
  • 资助金额:
    $ 17.59万
  • 项目类别:
T resident memory cells in arthritis
关节炎中的T常驻记忆细胞
  • 批准号:
    10684862
  • 财政年份:
    2019
  • 资助金额:
    $ 17.59万
  • 项目类别:
Bridging the gap between GWAS and mechanism in JIA
弥合 GWAS 和 JIA 机制之间的差距
  • 批准号:
    10064581
  • 财政年份:
    2018
  • 资助金额:
    $ 17.59万
  • 项目类别:
Bridging the gap between GWAS and mechanism in JIA
弥合 GWAS 和 JIA 机制之间的差距
  • 批准号:
    10675585
  • 财政年份:
    2018
  • 资助金额:
    $ 17.59万
  • 项目类别:
Bridging the gap between GWAS and mechanism in JIA
弥合 GWAS 和 JIA 机制之间的差距
  • 批准号:
    10622118
  • 财政年份:
    2018
  • 资助金额:
    $ 17.59万
  • 项目类别:
Administrative Core
行政核心
  • 批准号:
    10454987
  • 财政年份:
    2016
  • 资助金额:
    $ 17.59万
  • 项目类别:
Joint Biology Consortium Resource-based Center
联合生物学联盟资源中心
  • 批准号:
    10684880
  • 财政年份:
    2016
  • 资助金额:
    $ 17.59万
  • 项目类别:

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