Shared Resource 12: Nutrient and Phytochemical Analytics (NPASR)

项目摘要--营养和植物化学分析共享资源(NPASR) NPASR的使命是提供利基服务，以高质量的分析支持研究 烟草暴露的生物标记物、特定营养素或在食品中发现的大量抗癌植物化学物质 消息来源。NPASR主要支持分子致癌和化学预防(MCC)和癌症 控制(CC)计划，其中有强有力的研究检查饮食和烟草在癌症病因学中的作用， 预防和生存。NPASR的联席主任是史蒂文·克林顿博士，他是 癌症预防(临时联席主任、高级教员顾问；MCC联席负责人)和德文·彼得森，大四学生 食品、农业和环境科学学院的食品科学化学家。《公约》的具体目标 NPASR将：1)提供专业的、前沿的生物分析方法开发和定量分析 食品中的营养素和生物活性植物化学物质；2)对营养素进行有针对性的定量分析， 体外、体内和人体生物样品中的生物活性植物化学物质及其代谢物 使用高效液-质联用技术的研究；以及3)执行非靶向代谢组学和脂类组学服务 癌症相关研究。NPASR技术包括最先进的超高性能液体 色谱学(UHPLC)、质谱学(MS)和液相色谱三重四极杆MS/MS(LC-MS) MS/MS)。NPASR在当前的资金周期中增加了几个新的分析能力，包括：1)离子 移动硬件促进了广泛的半定量脂质组学平台；2)支持第二台MS仪器 非靶向代谢组学和脂类组学需求；以及3)最先进的MS/MS，以实现最高的灵敏度。在.期间 在当前的供资周期中，NPASR支持了49种出版物(2&>10个影响因素)、55个用户和6个NCI 奖助金，包括1个P50，3个R01，1个U19和1个U01，涉及所有五个CCC计划。这些努力和 出版物对定义暴露或摄入的生物标志物做出了贡献，并提供了对 植物化学物质的吸收、分布、代谢和排泄。最近，NPASR已经扩展到 靶向和非靶向代谢组学支持我们对烟草引起的个体癌症风险的理解 暴露、营养素、特定食物和饮食模式。满足OSUCC调查人员未来的需求 与OSUCCC的战略优先事项保持一致，NPASR将进一步支持，例如，代谢组学研究- 微生物组相互作用、代谢的遗传决定因素和烟草使用生物标记物。在下一次拨款期间 周期、脂质组学和生物活性脂质分析能力将得到扩展，以满足不断增长的用户的需求 所有五个项目的基础。NPASR为CCC成员评估食物、营养物质提供关键服务 以及从细胞培养和实验动物研究到人类的跨学科研究的致癌物质 试验和分子流行病学。NPASR的年度预算为387,249美元，但CCSG的请求是 74239美元。因此，NPASR利用广泛的机构支持，仅寻求19.2%的支持 CCSG基金。

PROJECT SUMMARY – NUTRIENT AND PHYTOCHEMICAL ANALYTICS SHARED RESOURCE (NPASR) The mission of the NPASR is to provide niche services supporting research with high quality assays for biomarkers of tobacco exposure, specific nutrients, or a vast array of anti-cancer phytochemicals found in food sources. NPASR primarily supports the Molecular Carcinogenesis and Chemoprevention (MCC) and the Cancer Control (CC) Programs, which have robust research examining the role of diet and tobacco in cancer etiology, prevention, and survivorship. The NPASR co-Directors are Drs. Steven Clinton, a translational researcher in cancer prevention (interim co-Director, Senior Faculty Advisor; MCC co-Leader) and Devin Peterson, a senior food science chemist in the College of Food, Agricultural, and Environmental Sciences. The Specific Aims of the NPASR are to: 1) provide expert, leading-edge bioanalytical method development and quantitative analysis of nutrients and bioactive phytochemicals in foodstuffs; 2) conduct targeted quantitative analysis of nutrients, bioactive phytochemicals and their metabolites in biological samples generated from in vitro, in vivo and human studies using HPLC-MS/MS techniques; and 3) perform untargeted metabolomics and lipidomics services for cancer-related studies. NPASR technologies include state-of-the-art ultra-high performance liquid chromatography (UHPLC), mass spectrometry (MS) and liquid chromatography triple quadrupole MS/MS (LC- MS/MS). NPASR has added several new analytical capabilities during the current funding cycle including: 1) ion mobility hardware facilitating a broad semi-quantitative lipidomics platform; 2) a second MS instrument to support untargeted metabolomics and lipidomics demand; and 3) a state-of-the-art MS/MS for utmost sensitivity. During the current funding cycle, the NPASR supported 49 publications (2 > 10 impact factor), 55 users, and 6 NCI grants, including 1 P50, 3 R01s, 1 U19 and 1 U01, involving all five CCC programs. These efforts and publications have contributed to defining biomarkers of exposure or intake, and provided novel insight into phytochemical absorption, distribution, metabolism, and excretion. More recently, NPASR has expanded into targeted and untargeted metabolomics supporting our understanding of individual cancer risk due to tobacco exposure, nutrients, specific foods, and dietary patterns. To meet future demands of OSUCC Investigators aligned with OSUCCC strategic priorities, NPASR will further support, for example, studies of metabolomic- microbiome interactions, genetic determinants of metabolism and tobacco use biomarkers. During the next grant cycle, lipidomics and bioactive lipid analytic capabilities will be expanded to meet the needs of a growing user base of all five programs. The NPASR provides a critical service for CCC members evaluating foods, nutrients and carcinogens that spans research disciplines from cell culture and experimental animal studies to human trials and molecular epidemiology. The annual budget of the NPASR is $387,249, yet the CCSG request is $74,239. As such, the NPASR leverages extensive institutional support and seeks only 19.2% support from CCSG funds.