Interactive Omics: Black raspberry metabolites and the oral microbiome in smokers

交互式组学：黑树莓代谢物和吸烟者的口腔微生物组

• 批准号: 8769882
• 负责人: STEVEN K CLINTON
• 金额: $ 63.68万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8769882
• 关键词: Adherence; Affect; American; Anthocyanins; Apoptosis; Area; Bacteria; Bioinformatics; Biological; Biological Availability; Biological Markers; Cancer Patient; Cell model; Characteristics; Clinical Research; Communities; Development; Diagnosis; Disease; Dose; Ecosystem; Epidemic; Exposure to; Food; Fruit; Future; Gene Expression Profile; Goals; Health; High Pressure Liquid Chromatography; Hour; Inflammation; Inflammatory Response; Interdisciplinary Study; Malignant Neoplasms; Mediating; Metabolism; Methodology; Metric; Microbial Biofilms; Molecular; Molecular Models; Operative Surgical Procedures; Oral; Oral cavity; Oral mucous membrane structure; Outcome Study; Patients; Phase III Clinical Trials; Phytochemical; Play; Population; Predisposition; Prevention strategy; Process; Processed Genes; Raspberries; Recurrence; Research; Research Design; Resolution; Retinoids; Risk; Rodent Model; Role; Saliva; Sensory; Shotguns; Smoker; Smoking; System; Testing; Time; TimeLine; Tobacco; Toxic effect; Treatment outcome; attenuation; base; cigarette smoke-induced; disorder prevention; high risk; liquid chromatography mass spectrometry; malignant mouth neoplasm; metabolomics; metagenomic sequencing; microbial; microorganism interaction; molecular modeling; never smoker; novel; oral bacteria; oral biofilm; oral carcinogenesis; oral microbiome; outcome forecast; pathogenic bacteria; point-of-care diagnostics; pre-clinical; public health relevance; smoking cessation; tobacco exposure; transcriptome sequencing

DESCRIPTION (provided by applicant): Oral cancer affects nearly 300,000 Americans each year and is a serious health concern due to its poor prognosis and high recurrence rate. Moreover, the worldwide tobacco epidemic is leading to thousands of new cases of oral cancer being diagnosed each year. Targeting preventive strategies for those at highest risk for oral cancer due to tobacco exposure is thus a high priority. Our ultimate goal is to employ a food-based cancer preventive strategy for high-risk populations that allows excellent long-term adherence and efficacy. The oral cavity is an open microbial ecosystem that plays host to over 700 species of bacteria that form health- compatible communities called biofilms. We have previously demonstrated that these biofilms are rapidly enriched for pathogenic bacteria in smokers, resulting in an early hyper-inflammatory response. Furthermore, smoking cessation reverses this pathogenic bacterial recolonization, demonstrating that smoking has a direct effect on the oral microbiome and may increase the risk for oral carcinogenic processes by disturbing normal host-bacterial interactions. Therefore, our central hypothesis is that a critical bi-directional interaction exists between oral bacteria and phytochemical-rich black raspberry food products, which ultimately results in attenuation of inflammation and amelioration of disease. First, we will determine the effect of black raspberry phytochemicals (BRBs) on community dynamics within oral biofilms by combining a longitudinal clinical study design and a novel BRB delivery system (BRB nectar) with the resolution provided by shotgun metagenomic sequencing and computational bioinformatics. Next, we will examine the effect of oral bacterial communities on metabolism of BRBs in current and never smokers. Targeted HPLC-MS/MS analysis will be used to identify known phenolics and metabolites, while untargeted UHPLC Q-TOF metabolome analysis will identify novel compounds. Finally, we will evaluate the efficacy of BRBs and their metabolites in reversing the effect of smoking on oral host-microbial interactions by combining a longitudinal clinical study design with a novel, high-throughput "dual RNA-Seq" methodology to simultaneously quantify both oral bacterial and oral mucosal transcriptome changes before and after exposure to BRB nectar. Following the successful completion of these Specific Aims, we will demonstrate for the first time the ability of BRBs to mitigate pathogenic metabolic process and gene expression patterns induced by cigarette smoke in a manner that supports oral carcinogenesis prevention strategies.

描述（由申请人提供）：口腔癌每年影响近30万美国人，由于其预后不良和复发率高，是一个严重的健康问题。此外，全球烟草流行导致每年诊断出数千例新的口腔癌病例。因此，针对那些由于烟草暴露而面临口腔癌最高风险的人制定预防战略是一个高度优先事项。我们的最终目标是为高风险人群采用基于食物的癌症预防策略，从而实现出色的长期依从性和疗效。口腔是一个开放的微生物生态系统，其中有超过700种细菌，这些细菌形成了称为生物膜的健康相容性群落。我们以前已经证明，这些生物膜在吸烟者中迅速富集致病菌，导致早期的高度炎症反应。此外，戒烟逆转了这种致病细菌的繁殖，表明吸烟对口腔微生物组有直接影响，并可能通过干扰正常的宿主-细菌相互作用增加口腔致癌过程的风险。因此，我们的中心假设是，口腔细菌和富含植物化学物质的黑树莓食品之间存在关键的双向相互作用，最终导致炎症的减弱和疾病的改善。首先，我们将通过将纵向临床研究设计和新型BRB递送系统（BRB花蜜）与鸟枪宏基因组测序和计算生物信息学提供的分辨率相结合，确定黑乌藨子植物化学物质（BRB）对口腔生物膜内群落动态的影响。接下来，我们将研究口腔细菌群落对当前吸烟者和从不吸烟者的BRB代谢的影响。有针对性的HPLC-MS/MS分析将用于识别已知的酚类化合物和代谢物，而非有针对性的UHPLC Q-TOF代谢组分析将识别新化合物。最后，我们将评估BRB及其代谢产物在逆转吸烟对口腔宿主-微生物相互作用的影响方面的功效，方法是将纵向临床研究设计与新型高通量“双重RNA-Seq”方法相结合，以同时量化暴露于BRB花蜜前后口腔细菌和口腔粘膜转录组的变化。在成功完成这些特定目标之后，我们将首次证明BRB能够以支持口腔癌预防策略的方式减轻香烟烟雾诱导的致病代谢过程和基因表达模式。