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Determining the impact of early adversity on the developing vertebrate brain

确定早期逆境对脊椎动物大脑发育的影响

基本信息

批准号：
10580285
负责人：
Erik Rolando Duboue
金额：
$ 45.75万
依托单位：
FLORIDA ATLANTIC UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-20 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10580285
关键词：
Address Adolescent Adult Aggressive behavior Anatomy Animals Anxiety Anxiety Disorders Atlases Back Behavior Behavioral Biological Biological Models Brain Brain imaging Brain region CRISPR/Cas technology Candidate Disease Gene Child Chronic Chronic stress Data Dependence Development Dissection Environmental Risk Factor Exposure to Feeds Fishes Genes Genetic Genetic Determinism Genetic Screening Genetic study Glucocorticoid Receptor Glucocorticoids Hormonal Human Image Imaging Techniques Investigation Larva Learning Life Life Experience Light Measures Mental disorders Microscopy Modeling Molecular Molecular Genetics Mutate Neuroanatomy Neurons Neurosecretory Systems Pathway interactions Physiological Physiology Predisposition Prevalence Regulation Reporter Schizophrenia Signal Transduction Stress Structure Substance abuse problem System Techniques Testing Time Transgenic Organisms Up-Regulation Work Zebrafish critical period early experience early life adversity experimental study genetic testing hands on research hormonal signals human disease insight mind control mutant neurodevelopment neurogenetics neuronal circuitry novel pediatric trauma post-traumatic stress programs stress related disorder tool transcriptomics two-photon undergraduate student

项目摘要

PROJECT SUMMARY In humans, chronic stress in children and adolescents has long lasting and debilitating effects that persist well into adulthood. Victims of childhood trauma have increased susceptibility to develop anxiety disorders such as post-traumatic stress (PTSD), increased propensity to substance abuse, hyper-aggression, and, in some cases, psychiatric disorders such as schizophrenia. Despite the prevalence of childhood trauma, little is known about how early life adversity impacts the development and function of the brain. This proposal utilizes a novel model of early life adversity (ELA) in the developmental and molecular neurogenetic system, the zebrafish. We will examine the impacts of ELA on hormonal, neuronal and genetic factors underlying brain development, and study how they are altered by ELA. The study utilizes powerful tools and approaches established in zebrafish, including whole-brain imaging of neuroanatomy and neuronal activity, transgenic reporters of glucocorticoid singling, and genetic screening using CRISPR/Cas9 technology at multiple developmental time points. Because of the high homology of genes and neuronal circuits underlying stress between fish and humans, we expect the findings from this study to reveal critical insight into what biological effects childhood trauma has on the developing brain.

项目总结