Development-Associated Linear Chromosome Segregation in Streptomyces
链霉菌中发育相关的线性染色体分离
基本信息
- 批准号:10579038
- 负责人:
- 金额:$ 40.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-20 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:ATP phosphohydrolaseATPase DomainActinobacteria classAlanineAnimal ModelAnthelminticsAntibiotic ResistanceAntibioticsAntifungal AgentsArchitectureBacteriaBacterial GenomeBacterial InfectionsBasic ScienceBindingBinding ProteinsBiotechnologyBiotinCell divisionCell physiologyCellular biologyCelluloseCentromereCharacteristicsChitinChromosome SegregationChromosomesComplexCytologyDNADepositionDevelopmentEcosystemEnvironmentEventFamilyFirmicutesGene ClusterGene MutationGenesGeneticGenomeGenomicsGoalsGrowthHealthHerbicidesHumanHyphaeImmunosuppressive AgentsKnowledgeLabelLaboratoriesLaboratory StudyLeadLeucine ZippersLifeLife Cycle StagesLigaseMalignant NeoplasmsMedicalMedicineModelingMorphologyMotorNatureNutrientOdorsOrganismPharmacologic SubstancePhysical condensationPlant RootsPlantsPolymersProductionPropertyProtein FamilyProtein SecretionProteinsQuality of lifeReproduction sporesRodScanningSideSoilStreptomycesStructureSystemTimeVeterinary Medicineanti-cancercombatcondensincostdaughter cellgenetic approachin vivomembermutantnext generationnovelnull mutationprotein protein interactionsegregationyeast two hybrid system
项目摘要
Project Summary
Streptomyces are ubiquitous filamentous sporulating soil bacteria, important in the environment
because they degrade and recycle the nutrients locked in many recalcitrant natural polymers in the
soil (e.g., cellulose and chitin) and produce the majority of a wide range of biologically active
compounds that are used extensively in human and veterinary medicine (e.g., antibiotic, antiviral,
anticancer, antitumor, antifungal, immunosuppressant and anthelmintic pharmaceuticals).
Streptomyces are also well known as one of the major producers of the compounds that give soil its
characteristic earthy odor. These organisms have large linear genomes. Little is known about the
architecture of the linear chromosome within the Streptomyces spore and the mechanisms which
govern its development-associated genome segregation and nucleoid condensation during spore
formation are poorly understood. The ultimate goal from the proposed basic research is to have a
better understanding of the novel cellular processes of these distinctive organisms of tremendous
medical importance.
The unusual mycelial life cycle and linear structure of the Streptomyces genome are likely to require
some unique solutions to the fundamental problems of genome segregation and condensation. The
first specific aim of the proposal is to characterize a novel small coiled-coil protein that is exclusive to
this group of organisms. A genetic approach was used to provide evidence that this protein is
involved in development-associated genome segregation. A proximity-labeling approach, with a
promiscuous biotin ligase fusion, is being used to identify proteins that interact with this novel protein
in vivo. Alanine-scanning and random PCR-directed mutageneses are being used to identify residues
important for protein-protein interaction with one known binding partner. The second specific aim is to
characterize type VII secretion system ATPase proteins. In addition to their functions in protein
secretion, the ATPase proteins are moonlighting in development-associated segregation and nucleoid
condensation. A genetic approach will be used to dissect the first type VII secretion ATPase to
identify the region(s) needed for genome segregation. A genetic approach will also be used to
determine the parts of both type VII secretion ATPases to identify the regions necessary for correct
nucleoid condensation in the spore. Mutants lacking genes encoding both type VII secretion ATPases
have genomes organized against the spore periphery (doughnut-shaped) instead of being condensed
as an ellipsoid nucleoid in the center of the spore.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JOSEPH R MC CORMICK其他文献
JOSEPH R MC CORMICK的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JOSEPH R MC CORMICK', 18)}}的其他基金
CHROMOSOME SEGREGATION IN A FILAMENTOUS BACTERIUM
丝状细菌中的染色体分离
- 批准号:
8036672 - 财政年份:2011
- 资助金额:
$ 40.11万 - 项目类别:
BIOCHEMICAL CHARACTERIZATION OF BACTERIAL CYTOKINESIS
细菌细胞分裂的生化特征
- 批准号:
6739235 - 财政年份:2004
- 资助金额:
$ 40.11万 - 项目类别:
DEVELOPMENTAL GENE REGULATION IN STREPTOMYCES COELICOLOR
蓝色链霉菌的发育基因调控
- 批准号:
3043937 - 财政年份:1991
- 资助金额:
$ 40.11万 - 项目类别:
DEVELOPMENTAL GENE REGULATION IN STREPTOMYCES COELICOLOR
蓝色链霉菌的发育基因调控
- 批准号:
3043936 - 财政年份:1990
- 资助金额:
$ 40.11万 - 项目类别:
DEVELOPMENTAL GENE REGULATION IN STREPTOMYCES COELICOLOR
蓝色链霉菌的发育基因调控
- 批准号:
3043935 - 财政年份:1989
- 资助金额:
$ 40.11万 - 项目类别:
相似海外基金
CRYSTAL STRUCTURE OF ADP COMPLEX OF ATPASE DOMAIN OF CHAPERONE HSC66
伴侣HSC66的ATP酶域ADP复合物的晶体结构
- 批准号:
6119556 - 财政年份:1999
- 资助金额:
$ 40.11万 - 项目类别: