Suppression of Host-Seeking Ixodes scapularis Abundances and Interruption of Pathogen Transmission Through Orally Delivered Systemic Acaricide Treatment of White-tailed Deer and Peromyscus spp.

1项目概要-威廉姆斯 2 RFA-CK-22-004 3 4.拟议的研究将口服现有的市售杀螨剂制剂， 5系统治疗肩突硬蜱的主要生殖宿主（白尾鹿）和主要病原体 6个水库（小型啮齿动物，重点是鹿鼠属）无论是单独还是组合。我们将使用 7 Cydectin®（莫昔克丁），人类食用的停药期为0天，用于包衣整个玉米， 8剂量鹿，并将使用Kaput®（0.005%氟虫腈）在三个不同的地点给小型啮齿动物剂量， 9种已知的替代陆生哺乳动物宿主的疾病。具体目标包括：（1）评估 图10该策略在抑制伯氏疏螺旋体感染的宿主寻找I中的功效和再现性。 在多个高风险环境中有11例肩胛肌撕裂。2)优化和规范实施 12系统杀螨剂治疗白尾鹿和鹿鼠属。来减少大量的寻找宿主的行为 13 I.感染了人类病原体的肩胛骨。3)制定标准作业程序， 14系统性杀螨宿主治疗，供房主或病媒控制专业人员使用， 15个社区有暴露于莱姆病螺旋体和其他I.肩胛骨传播的病原体 16这项工作的目的是研究系统杀螨宿主靶向的有效性。 17治疗作为一种综合蜱虫管理策略，可以在全镇范围内实施，类似于 18个蚊虫防治项目。在后勤方面，获得进入许多私人财产的机会进行蜱虫管理将 19挑战然而，白尾鹿的家通常包含许多私人财产， 20容易跨越人类衍生的财产边界。病媒控制专业人员或房主将 21只需要在几个地点建立喂养站，在春季和秋季给鹿服用莫昔克丁 22成人I.肩胛肌活动达到峰值，有效地中断蜱虫的生命周期。氟虫腈诱饵可能 23在自愿的私人财产上或由病媒控制专业人员或房主自己分发， 24目标少年I.肩胛肌对小型啮齿动物，进一步抑制丰度和减少病原体感染 25在载体和水库。此外，通过基于所提出的结果的建模工作， 26.将扩大战略，向不同生态和气候区域的利益攸关方提供以下信息： 27.各种干预措施的最佳组合，可以协同促进短期到长期的成本效益， 28采用病媒综合管理办法有效和可持续地控制蜱媒病原体。此外， 将公布29份可供公众查阅的文件，其中载有使用这一战略的标准作业程序 30并在网上提供，将面向房主和病媒控制专业人员。这 对主要生殖宿主和主要病原体储库进行系统治疗的联合策略， 32显着的潜力是非常有效的抑制宿主寻求丰富的病原体感染的I。 33肩胛肌在更大的区域，如在整个城镇的规模。

1 Project Summary-Williams 2 RFA-CK-22-004 3 4 The proposed research will orally deliver existing commercially available acaricide formulations to 5 systemically treat the major Ixodes scapularis reproductive host (white-tailed deer) and major pathogen 6 reservoir (small rodents with a focus on Peromyscus spp.) both singly and in combination. We will use 7 Cydectin® (moxidectin), which has a 0-day withdrawal period for human consumption, to coat whole corn to 8 dose deer and will use Kaput® (0.005% fipronil) to dose small rodents at three different locations with varying 9 known diversities of alternative terrestrial mammalian hosts. Specific aims include: 1) Evaluation of the 10 efficacy and reproducibility of this strategy in the suppression of Borrelia burgdorferi-infected host-seeking I. 11 scapularis nymphs in multiple high-risk settings. 2) Optimization and standardization of the implementation of 12 systemic acaricide treatment of white-tailed deer and Peromyscus spp. to reduce the abundance of host-seeking 13 I. scapularis nymphs infected with human pathogens. 3) Development of standard operating procedures for 14 systemic acaricidal host treatment to be used by homeowners or vector control professionals within 15 communities at risk for exposure to Lyme disease spirochetes and other I. scapularis-borne pathogens. 16 The objective of this work is to investigate the effectiveness of systemic acaricidal host-targeted 17 treatment as an integrated tick management strategy that can be implemented on a town-wide scale, similar to 18 mosquito control efforts. Logistically, procuring access to numerous private properties for tick management will 19 prove challenging. However, white-tailed deer home ranges often contain numerous private properties and they 20 readily traverse human-derived property boundaries. Vector control professionals or homeowners would then 21 only need to establish feeding stations at a few locations to dose deer with moxidectin during the spring and fall 22 adult I. scapularis activity peaks to effectively interrupt the tick life cycle. Fipronil-laced bait could be 23 distributed on willing private properties or by vector control professionals or the homeowners themselves to 24 target juvenile I. scapularis on small rodents, further suppressing abundances and reducing pathogen infection 25 in both vector and reservoir. Additionally, through modeling efforts based on results from the proposed, this 26 strategy will be expanded to provide information to stakeholders in different ecological and climatic regions on 27 the optimum combination of interventions that can synergistically contribute to the short- to long-term, cost- 28 effective and sustainable control of tick-borne pathogens in an integrated vector management approach. Also, a 29 publicly accessible document containing standard operating procedures for use of this strategy will be published 30 and made available online that will be geared toward homeowners and vector control professionals. This 31 combined strategy of systemic treatment of both the major reproductive host and major pathogen reservoir has 32 significant potential to be highly effective in suppression of host-seeking abundances of pathogen-infected I. 33 scapularis over larger areas such as at a town-wide scale.