Suppression of Host-Seeking Ixodes scapularis Abundances and Interruption of Pathogen Transmission Through Orally Delivered Systemic Acaricide Treatment of White-tailed Deer and Peromyscus spp.

1个项目摘要--Williams 2 RFA-CK-22-004 3. 4拟议的研究将以口头方式将现有的商用杀螨剂配方提供给 5系统治理肩部主要生殖宿主(白尾鹿)及主要病原菌 6水库(小型啮齿动物，主要集中在Permyscus spp.)既有单独的，也有组合的。我们将使用 7有0天撤除期供人类食用的Cydectin®(莫西菌素)，将整个玉米涂抹到 8剂量鹿，并将使用KAPUT®(0.005%氟虫腈)在三个不同的位置给小啮齿动物注射不同的剂量 9另类陆生哺乳动物宿主的已知多样性。具体目标包括：1)评估 10该策略在抑制伯氏疏螺旋体感染的寻找宿主I中的有效性和重复性。 多个高危环境中的11名肩胛若虫。2)优化和规范实施 12全身杀螨剂对白尾鹿和青霉的药效。为了减少寻找宿主的丰富性 13.感染人体病原体的肩部若虫。3)制定标准操作程序 14房主或病媒控制专业人员将使用的系统性杀螨剂 15个社区面临接触莱姆病、螺旋体和其他由肩周炎传播的病原体的风险。 16这项工作的目的是调查全身杀螨宿主靶向的有效性。 17作为可在全镇范围内实施的综合扁虱管理战略进行治疗，类似于 18项灭蚊工作。在后勤方面，获得大量私人物业的使用权以进行蚊虫管理将 事实证明，19个国家具有挑战性。然而，白尾鹿的养殖场往往包含许多私人财产，它们 20可以很容易地跨越人类衍生的财产边界。然后病媒控制专业人员或房主 21只需要在几个地点建立饲养站，在春秋两季给鹿注射莫西菌素 22个成人的肩胛肌活动高峰，有效地中断了扁虱的生命周期。氟虫腈诱饵可能是 23分发到有意愿的私人物业或由病媒控制专业人员或房主自己分发到 24靶向小鼠肩关节幼虫，进一步抑制丰度，减少病原体感染 在媒介和水库中均为25。此外，通过基于建议的结果的建模工作，这 26将扩大战略，向不同生态和气候区域的利益攸关方提供信息 27能够协同促进短期到长期成本的干预措施的最佳组合-- 28采用病媒综合管理办法，有效和可持续地控制壁虱传播的病原体。此外，还有一个 将公布29份载有使用这一战略的标准操作程序的可公开查阅的文件 30并在网上提供，面向房主和病媒控制专业人士。这 31对主要繁殖宿主和主要病原库进行系统治疗的综合策略 32在高效抑制病原菌感染的I. 33个肩胛骨分布在更大的区域，例如全镇范围内。

1 Project Summary-Williams 2 RFA-CK-22-004 3 4 The proposed research will orally deliver existing commercially available acaricide formulations to 5 systemically treat the major Ixodes scapularis reproductive host (white-tailed deer) and major pathogen 6 reservoir (small rodents with a focus on Peromyscus spp.) both singly and in combination. We will use 7 Cydectin® (moxidectin), which has a 0-day withdrawal period for human consumption, to coat whole corn to 8 dose deer and will use Kaput® (0.005% fipronil) to dose small rodents at three different locations with varying 9 known diversities of alternative terrestrial mammalian hosts. Specific aims include: 1) Evaluation of the 10 efficacy and reproducibility of this strategy in the suppression of Borrelia burgdorferi-infected host-seeking I. 11 scapularis nymphs in multiple high-risk settings. 2) Optimization and standardization of the implementation of 12 systemic acaricide treatment of white-tailed deer and Peromyscus spp. to reduce the abundance of host-seeking 13 I. scapularis nymphs infected with human pathogens. 3) Development of standard operating procedures for 14 systemic acaricidal host treatment to be used by homeowners or vector control professionals within 15 communities at risk for exposure to Lyme disease spirochetes and other I. scapularis-borne pathogens. 16 The objective of this work is to investigate the effectiveness of systemic acaricidal host-targeted 17 treatment as an integrated tick management strategy that can be implemented on a town-wide scale, similar to 18 mosquito control efforts. Logistically, procuring access to numerous private properties for tick management will 19 prove challenging. However, white-tailed deer home ranges often contain numerous private properties and they 20 readily traverse human-derived property boundaries. Vector control professionals or homeowners would then 21 only need to establish feeding stations at a few locations to dose deer with moxidectin during the spring and fall 22 adult I. scapularis activity peaks to effectively interrupt the tick life cycle. Fipronil-laced bait could be 23 distributed on willing private properties or by vector control professionals or the homeowners themselves to 24 target juvenile I. scapularis on small rodents, further suppressing abundances and reducing pathogen infection 25 in both vector and reservoir. Additionally, through modeling efforts based on results from the proposed, this 26 strategy will be expanded to provide information to stakeholders in different ecological and climatic regions on 27 the optimum combination of interventions that can synergistically contribute to the short- to long-term, cost- 28 effective and sustainable control of tick-borne pathogens in an integrated vector management approach. Also, a 29 publicly accessible document containing standard operating procedures for use of this strategy will be published 30 and made available online that will be geared toward homeowners and vector control professionals. This 31 combined strategy of systemic treatment of both the major reproductive host and major pathogen reservoir has 32 significant potential to be highly effective in suppression of host-seeking abundances of pathogen-infected I. 33 scapularis over larger areas such as at a town-wide scale.