Novel Transparent, Ultra-soft Neuroelectrode Arrays Based on Nanomeshing Conventional Electrode Materials SUPPLEMENT

摘要 我们的母公司奖项RF1NS118301的目标是开发和验证概念验证、纳米网格- 至少256个基于微电极、透明、超软、高密度(纳米网格)的阵列 高性能纳米网格微电极和伪迹拒绝无线数据链路。建议数 传播补充属于家长奖励范围内，但超出了原传播计划。 由于Parent奖的重点是为纳米网格阵列生成概念验证，我们在 在申请我们的母公司奖项时，建议的技术传播计划可能是 还为时过早。在过去的项目持续时间里，皮方的实验室已经建立了柔性的制造和测试 纳米网格微电极阵列(MEA)，有线连接高达256个通道。尽管家长奖 除了完全无线系统、有线连接之外，还旨在实现阵列的超柔软 纳米网格MEA仍然非常有用，特别适合于电生理学应用 同时进行活体成像，无论如何，动物都将被头部固定。这一传播不会 只有满足了社会上对这种电极阵列的一些迫切需求，它还会反过来提供有价值的 关于进一步的技术发展和如何最好地传播新的神经电子学的反馈 设备。因此，我们认为适当地继续我们现有的和/或 易于实现的有线、软纳米网格Meas，这将使三个最终用户实验室显著受益，同时 与家长奖高度协同。 这项赠款补充将通过以下方式大大促进预期的小额传播努力：(1)提供 两台设备将扩大和自动化软纳米网格的器件制造过程 MEAS，(2)购买材料和用品以建造更多的设备，(3)支持研究助理 在PI的实验室中进行关于正在传播的软纳米网状MEA的培训 处理/利用，以及(4)为我们的一个最终用户提供专用的录音系统，用于慢性疾病 实验。

Abstract The objective of our parent award RF1NS118301 is to develop and validate proof-of-concept, nanomesh- microelectrode-based, transparent, ultra-soft, high-density (NANOMESH) array with at least 256 high-performance nanomesh microelectrodes and artifact rejecting wireless data link. The proposed dissemination supplement is within the scope of the parent award, but beyond the original dissemination plans. As the focus of the parent award is to generate proof-of-concept for NANOMESH arrays, we decided at the time when applying for our parent award that a plan for dissemination of proposed technology in it may be premature. Over the past project duration, PI Fang’s lab has established the fabrication and testing of flexible nanomesh microelectrode arrays (MEAs) with up to 256-ch in wired connections. Although the parent award aims to achieve also ultra-softness for the arrays in addition to fully wireless system, wire-connected nanomesh MEAs are still highly useful and particularly suitable for electrophysiology applications with concurrent in vivo imaging, where the animals are going to be head-fixed anyway. This dissemination will not only fulfill some urgent needs in the community for such electrode arrays, it will also in turn, provide valuable feedback on both the further technology development and how to best disseminate novel neuroelectronic devices. Consequently, we feel it’s appropriate to pursue small dissemination efforts of our existing and/or easily-achievable wired, soft nanomesh MEAs, which will significantly benefit three end-user laboratories, while being highly synergistic with the parent award. This grant supplement will significantly facilitate the intended small dissemination efforts by (1) providing two pieces of equipment that will scale-up and automate the device fabrication process of soft nanomesh MEAs, (2) purchasing materials and supplies for building additional devices, (3) supporting research assistants in the PI’s lab to conduct training on the soft nanomesh MEAs being disseminated and their handling/utilization, and (4) supplying dedicated recording systems for one of our end-users for chronic experiments.