Multi-omics profiling of individual exosomes for their origin-tracing, biomarker discovery, and biological function characterization

对单个外泌体进行多组学分析，以进行起源追踪、生物标志物发现和生物功能表征

• 批准号: 10572470
• 负责人: Yuchao Chen
• 金额: $ 0.65万
• 依托单位: WELLSIM BIOMEDICAL TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-01 至 2022-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10572470
• 关键词: Address; Algorithms; Antibodies; Bar Codes; Biogenesis; Bioinformatics; Biological; Biological Assay; Biological Markers; Biological Process; Biomedical Technology; Blood specimen; Communities; DNA Sequence; Data; Detection; Disease; Early Diagnosis; Emulsions; Encapsulated; Expression Profiling; Gene Expression Profiling; Goals; Heterogeneity; High-Throughput DNA Sequencing; Human Cell Line; Individual; Label; Membrane Proteins; Messenger RNA; Methodology; Methods; Microfluidic Microchips; Modeling; Nucleic Acids; Oligonucleotides; Outcome; Performance; Phase; Population; Preparation; Prognosis; Proteins; Proteomics; RNA; Research; Resolution; Sampling; Small Business Innovation Research Grant; Speed; System; Techniques; Technology; Tissues; Untranslated RNA; Variant; base; biomarker discovery; disease diagnosis; exosome; extracellular vesicles; imaging modality; improved; individual variation; innovation; insight; interest; multiple omics; nanolitre; next generation sequencing; particle; protein profiling; targeted treatment; technological innovation; tool; transcriptomics

PROJECT SUMMARY / ABSTRACT Exosomes, the small extracellular vesicles (30-200 nm), are highly heterogeneous in biofluids. However, traditional bulk-level analysis approaches fail to represent their individual variations. A technique for multi-omics profiling of exosomes at single-particle resolution is of great interest to the exosome research field, but has yet to be developed. WellSIM proposes to develop and validate a method for multi-omics profiling of individual exosomes based on our exosome isolation system (EXODUS), a droplet-based microfluidic device (EXOSeq), and next-generation sequencing (NGS). Our proposed technique will be the first integrative proteomic and transcriptomic profiling of exosomes at the single-particle level, offering high-resolution multi-dimensional biological insights for exosome study and application.

项目摘要/摘要 胞外体是一种小的胞外小泡(30-200 nm)，在生物体液中具有高度的异质性。然而， 传统的批量分析方法不能代表它们的个体差异。一种多组学技术 在单粒子分辨率下分析外切体是外切体研究领域的一项重要内容，但尚未 有待开发。WellSIM建议开发和验证一种用于个人多组学分析的方法 基于我们的Exosome分离系统(ExoDus)的Exosome，一个基于液滴的微流体设备(Exoseq)， 和下一代测序(NGS)。我们提出的技术将是第一个整合的蛋白质组和 单颗粒水平外切体的转录图谱，提供高分辨率的多维 外切体研究和应用的生物学见解。