Equipment Supplement to 1R01GM141391-01A1 (Easily Used Kits to Evolve Reagents that Covalently Tag and Inactivate Proteins)

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Equipment Supplement for 1R01GM141391-01A1 (Easily Used Kits to Evolve Reagents that Covalently Tag and Inactivate Proteins) Foundation for Applied Molecular Evolution Steven Benner Abstract The Foundation for Applied Molecular Evolution (FfAME) holds a current NIGMS R01 research award (R01GM141391, Easily Used Kits to Evolve Reagents that Covalently Tag and Inactivate Proteins). Under this award, FfAME is requesting supplemental equipment funds that will be used to replace a 16-year-old BioRad PMI phosphorimager, an instrument that serves as the workhorse for the R01 project. The main goal of the funded R01 grant is to utilize FfAME’s artificially expanded genetic alphabet (AEGIS) to develop a platform to create receptors, ligands, and catalysts on demand. These molecules can be used to covalently modify, tag, and destroy proteins that are chosen by researchers and clinicians to target and solve many scientific, diagnostics, and disease management problems. This administrative supplement, if granted, will facilitate the purchase of an Amersham Typhoon Biomolecular Imager, which will replace the BioRad PMI and will serve a critical role in the success of the funded R01 project.