Multimetallic Catalysis in Biology and Synthesis

项目总结 提出这项行政补充申请是为了资助购买微型光纤 UV/可见光/近红外光谱仪，附带软件和采样附件。这台设备 将显著加快父奖项的研究，该奖项的目标是推动多金属 通过解决公开的科学问题和关于(A)仿生模型的挑战来实现催化 金属酶激发的络合物，以及(B)用于有机合成方法学的非生物催化剂。 本赠款周期内的大部分研究计划侧重于专题(A)，即 将通过拟议采购一台Ocean-HDX-XR光谱仪以及随行提供的 来自Ocean Insights的采样工具。这一工具将补充现有的 研究网站，该网站提供Windows-95时代的UV/可见光光谱仪，没有近红外功能。不仅 Ocean-HDX-XR是否会针对这台老化仪器的灾难性故障提供安全保障并扩展 在近红外区的光谱窗口，但它也将有助于在惰性大气下的测量 通过使用Wifi连接可转移到手套盒中。添加DIP探头附件将 还有助于反应中间体的现场反应监控和表征。案例研究包括 重点介绍了这些新增功能在了解反应途径方面的潜在优势 一氧化二氮还原酶的CuZ部位，好氧一氧化碳的Mo/Cu活性部位 脱氢酶和其他金属酶在父母奖下进行研究。

PROJECT SUMMARY This administrative supplement request is proposed to fund procurement of a miniature fiber-optic UV/visible/near-IR spectrometer with accompanying software and sampling accessories. This equipment will significantly accelerate research in the parent award, whose goals are to advance multimetallic catalysis by addressing open scientific questions and challenges regarding (a) biomimetic model complexes inspired by metalloenzymes, and (b) abiological catalysts for organic synthesis methodology. A majority of the research plan during the current grant cycle focused on topic (a), which is the one that will be aided by the proposed procurement of an Ocean-HDX-XR spectrometer with accompanying sampling tools from Ocean Insights. This instrument will complement existing core facilities at the research site, which offer a Windows-95 era UV/visible spectrometer with no near-IR capabilities. Not only will the Ocean-HDX-XR provide security against catastrophic failure of this aging instrument and expand the spectral window in the near-IR region, but it will also facilitate measurements under inert atmosphere by being transferable into gloveboxes using Wifi connectivity. The addition of a dip probe accessory will also facilitate in-situ reaction monitoring and characterization of reactive intermediates. Case studies are presented that highlight the potential benefits of these added capabilities to understand reaction pathways of the CuZ site of nitrous oxide reductase, the Mo/Cu active site of aerobic carbon monoxide dehydrogenase, and other metalloenzymes being studied under the parent award.