The effect of the microbiota on male Aedes aegypti life history traits

微生物群对雄性埃及伊蚊生活史性状的影响

• 批准号: 10574165
• 负责人: Sarah M Short
• 金额: $ 23.63万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-11-22 至 2024-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10574165
• 关键词: Accounting; Adult; Aedes; Affect; Bacteria; Biology; Body Size; Cessation of life; Communities; Complex; Copulation; Culicidae; Data; Dengue Virus; Development; Dietary Supplementation; Disease; Exposure to; Fat Body; Female; Gastrointestinal tract structure; Gland; Gnotobiotic; Goals; Insecta; Insemination; Investigation; La Crosse virus; Laboratories; Length; Location; Longevity; Male Sterility; Male Sterilizations; Measurement; Methods; Microbe; Mosquito Control; Mosquito-borne infectious disease; Nutrient; Organ; Partner in relationship; Pathogenicity; Physiology; Population; Proteins; Quality of life; Reproduction; Reproductive History; Role; Scourge; Seminal fluid; Sexual sterilization; Site; Sterility; Target Populations; Testing; Time; Tissues; Transcript; United States; Water; West Nile; Wing; Work; Zika Virus; disease transmission; fitness; fungus; immune system function; improved; insight; life history; male; microbial; microbiota; novel; novel strategies; offspring; prevent; reproductive; reproductive success; sperm cell; sterile insect technique; success; trait; transcriptome; transmission process; vector control

Project Summary: Mosquitoes transmit multiple diseases worldwide accounting for millions of disease cases and hundreds of thousands of deaths each year. The United States is not immune to the scourge of mosquito-borne disease, as mosquitoes in the USA can transmit dengue and Zika viruses as well as West Nile and La Crosse encephalitis virus. Mosquitoes are also a substantial nuisance that reduce quality of life and enjoyment of outdoor activity. Control of mosquito populations is the primary way we prevent disease transmission and nuisance issues. Many new methods of mosquito population reduction involve a modified version of a method called sterile insect technique (SIT) in which males are made reproductively sterile in a laboratory and released to mate with wild females. This results in zero offspring and a reduction in the population of that targeted mosquito species. SIT requires mass rearing of male mosquitoes in large rearing facilities and these males must be healthy and able to compete with wild males for mates. Here, we propose to investigate the potential impact the mosquito bacterial microbiota (i.e. bacteria in the mosquito body) may have on male Aedes aegypti mosquito traits that are relevant for SIT including longevity, body size, and ability to compete for mates. We will also investigate the way the microbiota affects the transcriptome of males. This work will be the first in-depth investigation into the role of the microbiota in male mosquito fitness traits and will provide key insight into ways the microbiota might be leveraged to improve SIT for mosquito control.

项目概要： 蚊子在全世界传播多种疾病，造成数百万病例， 每年有成千上万的人死亡。美国也不能幸免于 蚊子传播的疾病，因为美国的蚊子可以传播登革热和寨卡病毒， 以及西尼罗河和拉克罗斯脑炎病毒。蚊子也是一个很大的麻烦 这降低了生活质量和户外活动的乐趣。控制蚊子数量是 这是我们预防疾病传播和滋扰问题的主要方式。许多新的方法 减少蚊子数量的方法包括一种叫做不育昆虫的改良方法 一种技术（SIT），在实验室中使雄性生殖不育，然后释放到 与野生雌性交配这导致零后代和人口减少， 目标蚊子种类。SIT需要在大型饲养中大量饲养雄性蚊子 这些雄性必须是健康的，能够与野生雄性竞争配偶。在这里， 我们建议调查蚊子细菌微生物群（即细菌）的潜在影响。 蚊子身体）可能具有与SIT相关的雄性埃及伊蚊蚊子特征 包括寿命、体型和求偶能力。我们也会调查方式 微生物群影响雄性的转录组。这项工作将是第一次深入 调查微生物群在雄性蚊子健康特征中的作用，并将提供关键的 深入了解微生物群可能被利用来改善SIT以控制蚊子的方式。