Novel functions for Sm-class RNAs in the regulation of gene expression

Sm 类 RNA 在基因表达调控中的新功能

• 批准号: 10574621
• 负责人: Demian Cazalla
• 金额: $ 38.45万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-16 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10574621
• 关键词: 3' Untranslated Regions; Base Pairing; Binding; Binding Sites; Biochemical; Biological Models; Cells; Code; Disease; Eukaryota; Gene Expression; Gene Expression Regulation; Genetic; Goals; Herpesviridae; Homeostasis; Laboratories; Link; Mammalian Cell; Mechanics; Mediating; Messenger RNA; MicroRNAs; Molecular Biology; Nuclear; Primates; RNA; RNA Binding; RNA Processing; RNA Splicing; Research; Ribonucleoproteins; Saimiriine Herpesvirus 2; Small Nuclear RNA; Spider Monkey; Techniques; Untranslated RNA; Viral; Viral Cancer; Virus Diseases; Work; flexibility; genome-wide; in vivo; interest; mRNA Precursor; novel; particle; snRNP Structural Core Protein

Summary The overarching goal of my laboratory is to define the functions and mechanisms of a group of non- coding RNAs (ncRNAs): the small nuclear, U-rich RNAs (snRNAs). Cellular snRNAs associate with Sm proteins to form ribonucleoprotein particles (RNPs) that are essential components of cellular machineries responsible for the precursor messenger RNA (pre-mRNA) processing steps of splicing and 3¢-end formation, and their perturbation underlies several diseases. Recent research from my lab and others suggests that this group of ncRNAs is more functionally diverse than previously thought and that the canonical functions of snRNAs in splicing and 3¢-end processing constitute only the tip of the iceberg. Using Herpesvirus saimiri (HVS) as a model system, we recently showed that snRNAs perform functions beyond pre-mRNA processing. This primate g-herpesvirus expresses seven snRNAs in latently infected cells called HSURs (Herpesvirus saimiri U-rich RNAs). We have focused on two of these ncRNAs, HSURs 1 and 2, because they are the most highly conserved HSURs among different HVS groups and the only two expressed by the related Herpesvirus ateles. HSUR1 and HSUR2 basepair with host microRNAs (miRNAs) and target one miRNA, miR-27, for degradation. Work from our laboratory showed that HSUR2 also basepairs with host mRNAs and links bound miRNAs with specific mRNAs to destabilize them. This constitutes the first example of an snRNA function after pre-mRNA processing. We also developed iRICC, a technique to determine RNA binding partners of a single RNA of interest and the sequences mediating RNA-RNA interactions in vivo. Using iRICC we showed that HSUR2 binding sites reside mostly in the 3¢ untranslated regions (3¢UTRs) of target mRNAs. We also showed that HSUR2 does not present a “seed” or specialized region that is used to interact with most targets, but rather acts as a flexible adaptor that interacts through different base-pairing arrangements with different mRNAs. iRICC revealed that HSUR1 profusely binds to coding sequences in addition to 3¢UTRs of target mRNAs. These findings suggest that HSUR1 might regulate host gene expression through mechanisms different from those employed by HSUR2. Finally, we have also developed an approach for identifying low-abundance snRNAs and discovered that mammalian cells express previously uncharacterized snRNAs. We will use a combination of genetic, genome-wide, molecular biology and biochemical approaches to further characterize mechanical aspects of HSUR1 and HSUR2 function and to functionally characterize novel cellular snRNAs. This work will advance our understanding of the mechanisms underlying the regulation of gene expression by this class on ncRNAs during viral infection. It will also illuminate novel mechanisms of gene regulation in the broad range of eukaryotes that express snRNAs.

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