Core 3: The Affinity Reagent Characterization Core

核心 3：亲和试剂表征核心

• 批准号: 10573250
• 负责人: ANDREW N HOOFNAGLE
• 金额: $ 49.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10573250
• 关键词: Affinity; Alzheimer' s Disease; Alzheimer' s disease related dementia; Antibodies; Applications Grants; Autoantibodies; Binding; Biological Assay; Biological Markers; Body Fluids; Brain imaging; Cell Separation; Cerebrospinal Fluid; Clinical; Column Chromatography; Complex Mixtures; Deposition; Digestion; Elements; Ensure; Family; Goals; Human; Hybridomas; Image; Immunoassay; Investigation; Iowa; Label; Laboratories; Lipoproteins; Liquid Chromatography; Modernization; Monoclonal Antibodies; Mus; Natural regeneration; North America; Oryctolagus cuniculus; Patients; Peptides; Plasma; Proteins; Proteolysis; Reagent; Reproducibility; Research; Research Personnel; Running; Sampling; Serum; Specificity; Techniques; Technology; Thyroglobulin; Time; Tissues; Trypsin; Universities; Washington; assay development; data dissemination; design; direct patient care; experimental study; extracellular vesicles; human disease; imaging study; improved; instrument; interest; mass spectrometer; molecular mass; next generation; novel; particle; tandem mass spectrometry; tool; translational proteomics

Abstract Affinity reagents are essential to almost any modern scientific investigation of human disease. Antibodies are the most widely used family of affinity reagents and when cloned and produced in culture represent a powerful tool that can be continually regenerated. Many monoclonal antibodies boast strong interactions, slow off-rates, and good specificity. To supplement monoclonal antibodies, the Baker laboratory at the University of Washington has developed techniques to design small proteins de novo that bind molecules of interest. The overall goal of this Core entitled, “Affinity Regent Characterization Core,” is to provide the reagents needed for Projects 2 and 3 by (1) developing new monoclonal antibodies to be used in different assays, (2) labeling antibodies and novel small protein affinity reagents for use in CyTOF and brain imaging, and (3) carefully characterizing each reagent to ensure that different batches of affinity reagents behave similarly.

摘要 亲和试剂对于几乎任何现代人类疾病的科学研究都是必不可少的。抗体是 最广泛使用的亲和试剂家族，当克隆并在培养物中产生时， 这是一种可以不断再生的工具。许多单克隆抗体都具有很强的相互作用，缓慢的解离速率， 特异性好。为了补充单克隆抗体，贝克大学实验室 华盛顿已经开发出了新设计小蛋白质的技术，这些蛋白质可以结合感兴趣的分子。的 该核心的总体目标是“亲和试剂表征核心”， 项目2和3通过（1）开发新的单克隆抗体用于不同的测定，（2）标记 用于CyTOF和脑成像的抗体和新型小蛋白亲和试剂，以及（3）仔细地 表征每种试剂以确保不同批次的亲和试剂表现相似。