Core 3: The Affinity Reagent Characterization Core

核心 3：亲和试剂表征核心

基本信息

批准号：
10359190
负责人：
ANDREW N HOOFNAGLE
金额：
$ 48.47万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-15 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10359190
关键词：
Affinity Alzheimer&apos s Disease Alzheimer&apos s disease related dementia Antibodies Applications Grants Autoantibodies Binding Biological Assay Biological Markers Body Fluids Brain imaging Cell Separation Cerebrospinal Fluid Clinical Complex Mixtures Deposition Digestion Elements Ensure Family Goals Human Hybridomas Image Immunoassay Investigation Iowa Label Laboratories Lipoproteins Liquid Chromatography Modernization Monoclonal Antibodies Mus Natural regeneration North America Oryctolagus cuniculus Patients Peptides Plasma Proteins Proteolysis Reagent Reproducibility Research Research Personnel Running Sampling Serum Specificity Techniques Technology Thyroglobulin Time Tissues Trypsin Universities Washington assay development data dissemination design direct patient care experimental study extracellular vesicles human disease imaging study improved instrument interest mass spectrometer molecular mass next generation novel particle tandem mass spectrometry tool translational proteomics

项目摘要

Abstract Affinity reagents are essential to almost any modern scientific investigation of human disease. Antibodies are the most widely used family of affinity reagents and when cloned and produced in culture represent a powerful tool that can be continually regenerated. Many monoclonal antibodies boast strong interactions, slow off-rates, and good specificity. To supplement monoclonal antibodies, the Baker laboratory at the University of Washington has developed techniques to design small proteins de novo that bind molecules of interest. The overall goal of this Core entitled, “Affinity Regent Characterization Core,” is to provide the reagents needed for Projects 2 and 3 by (1) developing new monoclonal antibodies to be used in different assays, (2) labeling antibodies and novel small protein affinity reagents for use in CyTOF and brain imaging, and (3) carefully characterizing each reagent to ensure that different batches of affinity reagents behave similarly.

抽象的亲和力试剂对于几乎任何现代人类疾病的科学研究至关重要。抗体是使用最广泛的亲和力试剂家族，克隆和产生的文化代表强大的可以连续再生的工具。许多单克隆抗体具有强烈的相互作用，较慢的偏移速率，和良好的特异性。为了补充单克隆抗体，大学的贝克实验室华盛顿已经开发了设计从头开始结合感兴趣分子的小蛋白质的技术。这这个核心的总体目标标题为“亲和力摄政表征核心”，是为了提供所需的试剂项目2和3 by（1）开发新的单克隆抗体，用于不同的测定法，（2）标签用于细胞和脑成像的抗体和新型的小蛋白亲和力试剂，（3）仔细表征每种试剂以确保不同的亲和力试剂的行为类似。