A Synchrotron Radiation Structural Biology Resource

同步辐射结构生物学资源

• 批准号: 10578805
• 负责人: Aina E. Cohen
• 金额: $ 119.63万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10578805
• 关键词: Acute; Automatic Data Processing; Automation; Biomedical Research; Clinic; Collaborations; Collection; Communities; Complex; Computer software; Coupled; Cryoelectron Microscopy; Cryopreservation; Crystallization; Crystallography; Data; Data Analyses; Data Collection; Data Set; Dedications; Dehydration; Discipline; Documentation; Education and Outreach; Educational workshop; Fostering; Freezing; Generations; Goals; Humidity; Ice; In Situ; Individual; Institution; Instruction; Internet; Investigation; Light; Location; Manuals; Measurement; Membrane Proteins; Methodology; Methods; Molecular Biology; Monitor; Multiprotein Complexes; Peer Review; Performance; Process; Productivity; Protein Dynamics; Publications; Radiation; Raman Spectrum Analysis; Reaction; Recording of previous events; Research; Resolution; Resources; Roentgen Rays; Running; Sampling; Schools; Science; Services; Site; Source; Structure; Synchrotrons; System; Techniques; Technology; Time; Training; Vision; absorption; beamline; biological systems; computerized data processing; data structure; design; detector; experience; experimental study; flexibility; innovation; instrumentation; lectures; metalloenzyme; novel; online resource; operation; outreach; outreach program; programs; software development; spectroscopic imaging; structural biology; success; synchrotron radiation; temperature jump; tool; user-friendly; web portal; x-ray free-electron laser

Synchrotron radiation (SR) is an extremely bright and tunable x-ray source that enables forefront research in structural molecular biology (SMB). The goal of “A Synchrotron Structural Biology Resource" at the Stanford Synchrotron Radiation Lightsource (SSRL) is to provide users access to an integrated, mature SMB Resource with state-of-the-are synchrotron beam line facilities for macromolecular crystallography, x-ray absorption/emission/imaging spectroscopy and small angle x-ray scattering/diffraction, that form the three Resource Technical Operations Cores. Special emphasis is to create streamlined user access, provide dedicated user support and services, to train users, and to perform outreach on the scientific benefits of the capabilities of these specialized, yet mature facilities to the biomedical research community. Multiple innovative access mechanisms will be provided, that will include both single- and multiple-technique approaches to access the Cores, based on a transparent and robust external peer-review process. A multifaceted training and outreach program will include individual training, workshops, summer schools, on-the-road, and support clinic segments by experienced science and technical staff. A Resource web portal will provide streamlined scientific, technical, training, outreach and administrative material and other relevant information. A principal aim is to optimize and maintain, for reliable and forefront performance, experimental facilities and instrumentation, detectors, software and computing and network capabilities on the SMB Resource’s 8+ beam lines at SSRL, capitalizing on the high SR x-ray performance of SSRL’s 3rd generation storage ring SPEAR3, and at the MFX station at LCLS. A multi-facility proposal mechanism will facilitate access to complementary Cryo-EM and LCLS stations on-site at SLAC. With a specialized focus on user training, support and service, the SMB Resource will enable the scientific success for challenging and routine projects in the biomedical sciences.

同步辐射（SR）是一种非常明亮和可调谐的X射线源， 结构分子生物学（SMB）“同步加速器结构生物学”的目标是 资源”在斯坦福大学同步辐射光源（SSRL）是为用户提供访问 到一个集成的，成熟的SMB资源与国家的是同步加速器光束线设施， 大分子晶体学、X射线吸收/发射/成像光谱学和小 角X射线散射/衍射，形成三个资源技术操作核心。 特别强调的是创建简化的用户访问，提供专门的用户支持， 服务，培训用户，并就 这些专业的，但成熟的设施，以生物医学研究界。多个创新 将提供访问机制，包括单技术和多技术 在透明和强有力的外部同行审查的基础上， 过程一个多方面的培训和推广计划将包括个人培训，讲习班， 暑期学校，在路上，并支持诊所部分由经验丰富的科学和技术 工作人员一个资源门户网站将提供精简的科学、技术、培训、外联和 行政材料和其他有关资料。一个主要目标是优化和维护， 可靠和前沿的性能，实验设施和仪器，探测器， 在SSRL的SMB资源的8+光束线上的软件、计算和网络能力， 利用SSRL第三代储存环SPEAR 3的高SR X射线性能， 以及LCLS的MFX电台一个多设施提案机制将促进获得 补充Cryo-EM和LCLS站在SLAC现场。专注于用户 培训、支持和服务，中小企业资源将使科学成功， 和生物医学科学的常规项目。