A Synchrotron Radiation Structural Biology Resource

同步辐射结构生物学资源

• 批准号: 10578805
• 负责人: Aina E. Cohen
• 金额: $ 119.63万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10578805
• 关键词: Acute; Automatic Data Processing; Automation; Biomedical Research; Clinic; Collaborations; Collection; Communities; Complex; Computer software; Coupled; Cryoelectron Microscopy; Cryopreservation; Crystallization; Crystallography; Data; Data Analyses; Data Collection; Data Set; Dedications; Dehydration; Discipline; Documentation; Education and Outreach; Educational workshop; Fostering; Freezing; Generations; Goals; Humidity; Ice; In Situ; Individual; Institution; Instruction; Internet; Investigation; Light; Location; Manuals; Measurement; Membrane Proteins; Methodology; Methods; Molecular Biology; Monitor; Multiprotein Complexes; Peer Review; Performance; Process; Productivity; Protein Dynamics; Publications; Radiation; Raman Spectrum Analysis; Reaction; Recording of previous events; Research; Resolution; Resources; Roentgen Rays; Running; Sampling; Schools; Science; Services; Site; Source; Structure; Synchrotrons; System; Techniques; Technology; Time; Training; Vision; absorption; beamline; biological systems; computerized data processing; data structure; design; detector; experience; experimental study; flexibility; innovation; instrumentation; lectures; metalloenzyme; novel; online resource; operation; outreach; outreach program; programs; software development; spectroscopic imaging; structural biology; success; synchrotron radiation; temperature jump; tool; user-friendly; web portal; x-ray free-electron laser

Synchrotron radiation (SR) is an extremely bright and tunable x-ray source that enables forefront research in structural molecular biology (SMB). The goal of “A Synchrotron Structural Biology Resource" at the Stanford Synchrotron Radiation Lightsource (SSRL) is to provide users access to an integrated, mature SMB Resource with state-of-the-are synchrotron beam line facilities for macromolecular crystallography, x-ray absorption/emission/imaging spectroscopy and small angle x-ray scattering/diffraction, that form the three Resource Technical Operations Cores. Special emphasis is to create streamlined user access, provide dedicated user support and services, to train users, and to perform outreach on the scientific benefits of the capabilities of these specialized, yet mature facilities to the biomedical research community. Multiple innovative access mechanisms will be provided, that will include both single- and multiple-technique approaches to access the Cores, based on a transparent and robust external peer-review process. A multifaceted training and outreach program will include individual training, workshops, summer schools, on-the-road, and support clinic segments by experienced science and technical staff. A Resource web portal will provide streamlined scientific, technical, training, outreach and administrative material and other relevant information. A principal aim is to optimize and maintain, for reliable and forefront performance, experimental facilities and instrumentation, detectors, software and computing and network capabilities on the SMB Resource’s 8+ beam lines at SSRL, capitalizing on the high SR x-ray performance of SSRL’s 3rd generation storage ring SPEAR3, and at the MFX station at LCLS. A multi-facility proposal mechanism will facilitate access to complementary Cryo-EM and LCLS stations on-site at SLAC. With a specialized focus on user training, support and service, the SMB Resource will enable the scientific success for challenging and routine projects in the biomedical sciences.

同步加速器辐射（SR）是一种极其明亮且可调的X射线源，可实现最前沿 结构分子生物学（SMB）的研究。 “同步结构生物学的目标 资源“在斯坦福同步辐射光线（SSRL）的资源”是为用户访问 提供具有最新的同步光束线设备的集成，成熟的SMB资源 大分子晶体学，X射线抽象/发射/成像光谱和小 角度X射线散射/衍射，形成了三个资源技术操作核心。 特别重点是创建精简的用户访问，提供专用的用户支持和 服务，培训用户，并对能力的科学利益进行宣传 这些专门的但成熟的设施是生物医学研究界的。多重创新 将提供访问机制，其中包括单一和多技术 基于透明且强大的外部同行评审访问核心的方法 过程。多方面的培训和外展计划将包括个人培训，研讨会， 经验丰富的科学和技术的暑期学校，公路和支持诊所 职员。资源Web门户将提供精简的科学，技术，培训，外展和 行政材料和其他相关信息。主要目的是优化和维护， 为了获得可靠和最前沿的性能，实验设施和仪器，探测器， SMB资源在SSRL的8+梁线上的软件，计算和网络功能， 利用SSRL的第三代存储环Spear3的高SR X射线性能， 在LCLS的MFX站。多物种提案机制将有助于访问 SLAC的现场完全冷冻EM和LCLS站。专门关注用户 培训，支持和服务，SMB资源将为挑战带来科学成功 和生物医学科学中的常规项目。