Enable high-content live imaging in the study of constraint related morphome

点击翻译按钮获取中文摘要

PROJECT SUMMARY Brain cerebral developments are fundamental and impactful bio-behaviors under spatial constraints. Understanding their biophysical process and molecular mechanisms can be benefited using the emergent organoids and other 3D tissue models. We aim to characterize the morphomics of the complex cell organizations in these 3D tissue models through advanced imaging tools. This proposed equipment is a high-content, confocal, live imaging system, providing a technological leap in our current organoid analysis. The technological leap through this equipment will enable three major enhancements. 1) 3D deep imaging allows tracing tissue morphology and differentiation patterns. 2) Confocal live imaging can capture the dynamic translocation of individual cells, providing experimental data for time- dependent theoretical modeling. 3) High-content imaging makes it possible to scale up the experiments. In summary, this imaging system will help our current study seize new research opportunities and support the development of novel scientific ideas in the morphomic study of brain organoids.