Deposition of GBM invasion chip data to the Microphysiology Systems Database

将 GBM 入侵芯片数据沉积到微生理学系统数据库

• 批准号: 10382952
• 负责人: Yu Huang
• 金额: $ 5.06万
• 依托单位: UTAH STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10382952
• 关键词: 3-Dimensional; Brain Neoplasms; Cell Line; Chemicals; Data; Data Set; Database Management Systems; Deposition; Devices; Disease; Drug Kinetics; Environment; General Population; Glioblastoma; Grant; Individual; Malignant neoplasm of brain; Microfluidic Microchips; Modeling; Outcome Study; Patients; Perfusion; Pharmaceutical Preparations; Research; System; Time; United States National Institutes of Health; base; brain tissue; cancer cell; cell motility; cellular imaging; effective therapy; interstitial; microphysiology system

PROJECT SUMMARY Glioblastoma (GBM) is the most prevalent and aggressive primary malignant brain tumor. The patient median survival is only fifteen months, due to its chemotactic invasion into adjacent brain tissues through the 3D-confined interstitial space. The underlying mechanism is still poorly understood, and effective therapies are still lacking. This is due to the lack of adequate research platforms. There is currently no representation of this disease in the Microphysiology System Database. Through an active NIH grant study, we recently developed a microfluidic device to study GBM invasion. This device recapitulates the native environment for individual cancer cell migration through interstitial space. We further integrated the chemical perfusion system that can manipulate the chemotactic environment (e.g., chemical composition, absolute concentration, concentration gradient) in real-time. So far, we have collected a significant amount of cell images and migration data, in various chemotactic conditions. We also collected the pharmacokinetic data for a potential inhibitory drug. To render the data to the general public, we propose to re-format and deposit them in the Microphysiology System Database. We will also expand the dataset by collecting the pharmacokinetic data from additional patient-derived cell lines. This study's outcome will significantly enhance our understanding of the GBM invasion and promote the discovery of more effective therapy.

项目摘要 胶质母细胞瘤（GBM）是最常见和最具侵袭性的原发性脑恶性肿瘤 肿瘤由于其趋化性，患者的中位生存期仅为15个月。 通过3D限制的间隙侵入邻近脑组织。的 其潜在机制仍然知之甚少，并且仍然缺乏有效的治疗方法。 这是因为缺乏足够的研究平台。目前没有 在微生理学系统数据库中描述这种疾病。 通过一项积极的NIH资助研究，我们最近开发了一种微流体装置来研究 GBM入侵。这个装置重现了个体癌症的自然环境 细胞通过间隙迁移。我们进一步整合了化学灌注 可以操纵趋化环境的系统（例如，化学成分， 绝对浓度、浓度梯度）。到目前为止，我们已经收集了 大量的细胞图像和迁移数据，在各种趋化条件下。 我们还收集了潜在抑制药物的药代动力学数据。 为方便公众查阅，我们建议把资料重新格式化，然后存款 微生理学系统数据库我们还将通过收集 来自其他患者来源的细胞系的药代动力学数据。这项研究的结果 将大大提高我们对GBM入侵的理解，并促进 发现更有效的治疗方法