Protein Production Shared Resources Core

蛋白质生产共享资源核心

• 批准号: 10582667
• 负责人: CHARALAMPOS KALODIMOS
• 金额: $ 23.27万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10582667
• 关键词: Antineoplastic Agents; BAX gene; Bacteria; Binding; Biochemical; Biology; Biophysics; Calorimetry; Cancer Center Support Grant; Cell Culture Techniques; Cell Death; Cells; Cellular Stress; Chairperson; Chemicals; Childhood Cancer Genome Project; Chimeric Proteins; Chromatography; Cloning; Collaborations; Consultations; Cytoplasmic Tail; DNA Sequence Alteration; Department chair; Doctor of Philosophy; Documentation; Ensure; Equipment; Fermentation; Funding; Goals; Grant; Guanosine Triphosphate Phosphohydrolases; Human Resources; Immunologics; Insecta; Institution; Journals; Leadership; Ligands; Macrophage Colony-Stimulating Factor; Malignant Childhood Neoplasm; Malignant Neoplasms; Mammalian Cell; Manuscripts; Metabolism; Missense Mutation; Pharmaceutical Preparations; Population; Production; Protein Biochemistry; Proteins; Publications; Rana; Reagent; Recommendation; Recurrence; Research; Research Personnel; Resource Sharing; Resources; Review Committee; Robotics; Saint Jude Children' s Research Hospital; Savings; Science; Services; Signal Pathway; Structure; Surface Plasmon Resonance; System; TP53 gene; Technology; Testing; Therapeutic; Titrations; Universities; Update; Vision; Washington; Work; analytical ultracentrifugation; anticancer research; cost; cytokine; drug discovery; economic value; equipment acquisition; experience; improved; instrumentation; member; novel anticancer drug; pro-apoptotic protein; programs; protein expression; protein function; protein purification; repaired; response; screening services; structural biology; working group

PROJECT SUMMARY/ABSTRACT–Protein Technology Shared Resource The goal of the Protein Technology Shared Resource (PTSR) is to generate protein reagents and provide analytical instrumentation that enables SJCCC members to explore the biology and therapeutic consequences of altered protein function in cancer. The PTSR offers state-of-the-art technologies for protein expression and purification from bacteria, insect cells, and mammalian host systems, and provides expert consultation to SJCCC members regarding their needs for these services. The core has recently upgraded its protein expression pipeline by acquiring contemporary automated protein purification systems to enhance the quality of the final product and the documentation of its purity. The PTSR was founded and has been directed by Charles Rock, PhD, an expert in protein expression and purification. Moving forward, the leadership of the PTSR is transitioning in the next funding period to Charalampos Kalodimos, PhD (CBP), the new Chair of the Department of Structural Biology. Kalodimos has decades of experience in protein biochemistry and brings new energy and vision to enhancing the impact of the PTSR on SJCCC research. Currently, Rock and Kalodimos are co- directing the PTSR for one year to ensure a smooth transition in leadership. When new components are fully integrated into the PTSR, the resource will have 9 technical staff, 1 manager, and the Director (Kalodimos). The PTSR is a highly specialized Shared Resource that provides a relatively small but crucial service with a significant impact on the science of the SJCCC. The centralization of resources for protein production, purification, and analysis provides economic value to SJCCC members, and the PTSR can usually offer substantial savings even for commercially available proteins. Cytokines, for example, cost $10 to $30 per microgram commercially, whereas PTSR recently produced 250 micrograms of M-CSF for Kanneganti (CBP) for $0.50 per microgram, saving hundreds of thousands of dollars. Of the 26 manuscripts using the PTSR, 7 (27%) involved intraprogrammatic collaboration, and 4 (15%) involved interprogrammatic collaboration. Work involving the PTSR has had marked scientific impact, including publications from 4 of the 5 Programs, CBP (n=20), DBSTP (n=7), HMP (n=3), and NBTP (n=1). In the upcoming funding period, the PTSR will continue to offer bacterial, insect, and mammalian cell expression systems for protein production, supported by existing fermentation equipment and cell culture facilities. The transition in Directors will be completed, and services will be significantly expanded and improved, including expansion of the protein-expression capabilities and the integration of the molecular-interaction components. State-of-the art cloning and expression services will be augmented by the addition of a Hudson Robotics ProLink Protein Expression System capable of performing high-throughput expression screens of multiple clones, which will be operational by July 2018.

项目概要/摘要-蛋白质技术共享资源 蛋白质技术共享资源（PTSR）的目标是生成蛋白质试剂并提供 分析仪器，使SJCCC成员能够探索生物学和治疗结果 癌症中蛋白质功能的改变。PTSR提供了最先进的蛋白质表达技术， 从细菌，昆虫细胞和哺乳动物宿主系统中纯化，并为SJCCC提供专家咨询 了解他们对这些服务的需求。核心最近升级了它的蛋白质表达 通过收购现代自动化蛋白质纯化系统，以提高最终产品的质量， 产品及其纯度的文件。PTSR由查尔斯·洛克创立并执导， 博士，蛋白质表达和纯化专家。展望未来，PTSR的领导层正在过渡 在下一个资助期Charalampos Kalodimos，博士（CBP），该部门的新主席 结构生物学Kalodimos在蛋白质生物化学方面拥有数十年的经验， 愿景是增强PTSR对SJCCC研究的影响。目前，Rock和Kalodimos正在合作， 指导PTSR一年，以确保领导层的平稳过渡。当新组件完全 整合到PTSR，资源将有9名技术人员，1名经理和主任（Kalodimos）。的 PTSR是一种高度专业化的共享资源，它提供相对较小但至关重要的服务， 对SJCCC科学的影响。集中资源用于蛋白质生产、纯化和 分析为SJCCC成员提供了经济价值，PTSR通常可以提供大量的节省， 用于商业上可获得的蛋白质。例如，细胞因子的商业成本为每微克10至30美元， 而PTSR最近为Kanneganti（CBP）生产了250微克M-CSF，每微克0.50美元， 节省了数十万美元。在使用PTSR的26篇稿件中，7篇（27%）涉及 方案内协作，4项（15%）涉及方案间协作。工作涉及 PTSR具有显著的科学影响，包括5个项目中的4个项目的出版物，CBP（n=20），DBSTP （n=7）、HMP（n=3）和NBTP（n=1）。在即将到来的资助期内，PTSR将继续提供细菌， 用于蛋白质生产昆虫和哺乳动物细胞表达系统 设备和细胞培养设施。董事的过渡将完成，服务将大大增加。 扩大和改善，包括扩大蛋白质表达能力和整合的蛋白质， 分子相互作用成分。最先进的克隆和表达服务将得到加强， 增加了能够进行高通量分析的哈德逊Robotics ProLink蛋白表达系统 多个克隆的表达筛选，将于2018年7月投入使用。