Diabetes Models Phenotyping

糖尿病模型表型分析

• 批准号: 10583248
• 负责人: Irfan J Lodhi
• 金额: $ 13.34万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10583248
• 关键词: Affect; Animal Model; Animals; Biochemical; Biological Assay; Blood pressure determination; Body Composition; Cell Culture Techniques; Cells; Cholesterol; Clinical; Communities; Consultations; Data; Diabetes Mellitus; Disease; Dual-Energy X-Ray Absorptiometry; Energy Metabolism; Environment; Experimental Designs; Feedback; Funding; Glucose; Glucose Clamp; Goals; Human; Hyperinsulinism; In Vitro; Indirect Calorimetry; Laboratories; Measures; Metabolic; Metabolic Diseases; Metabolism; Methodology; Modeling; Modification; Molecular; Mus; Nonesterified Fatty Acids; Obesity; Organ; Organism; Paper; Patient Care; Phenotype; Productivity; Publications; Reproducibility; Research; Research Personnel; Research Project Grants; Sampling; Scanning; Scientist; Serum; Services; Surveys; Systemic disease; Technology; Testing; Time; Tissues; Training; Translating; Triglycerides; United States National Institutes of Health; Universities; Washington; clinical application; cost effective; cost effectiveness; diabetic; experience; experimental study; glucose tolerance; improved; innovation; insight; instrumentation; insulin tolerance; meetings; member; mouse model; novel; response; service utilization; translational potential

PROJECT SUMMARY: Diabetes Models Phenotyping Core The Diabetes Models Phenotyping (DMP) Core is an important component of the Washington University Diabetes Research Center (DRC). The long-term goal of the DMP Core is to enhance diabetes- related research at Washington University using sophisticated services and technologies with the potential to contribute to discoveries that can be translated to novel treatments for diabetes and related disorders. The DMP Core has three objectives: 1) To provide phenotyping services to DRC members to both facilitate and enhance the cost-effectiveness of NIH-funded diabetes/metabolism-related research; 2) To provide expert consultation and training to DRC members in experimental design and characterization of mouse models relevant to diabetes, obesity and associated metabolic diseases; and 3) To develop new research capabilities to enhance the ability of DRC members to perform diabetes and metabolic research. The Core offers a broad range of animal phenotyping services, including a) body composition analysis using EchoMRI and/or DEXA scan; b) indirect calorimetry to measure energy expenditure; c) glucose and insulin tolerance testing; d) blood pressure determinations; and e) biochemical analyses of serum and tissues to measure glucose, triglycerides, free fatty acids and cholesterol. Meeting a key need in the diabetes research community at Washington University, the Core provided services to 44 different DRC member laboratories during the past funding period, including 11 P&F recipients. The services utilized by these investigators over this time frame include biochemical assays of serum (>4,800 samples) and tissues (>1,100 samples), body composition determinations for >8500 mice, and indirect calorimetry assessments in >2,400 mice, to mention the services in greatest demand. These analyses supported the publication of 130 papers. The Core continually evolves in response to user needs through elimination of some services and addition of others. New and expanded services include hyperinsulinemic-euglycemic clamps, thermogenic phenotyping, and expanded training and consultation. Together, DMP Core services continue to help DRC investigators contribute important scholarly advances with substantial potential for translation to improved care for patients suffering from diabetes and related complications.

项目总结：糖尿病模型表型核心 糖尿病模型表型分析（Diabetes Models Phenotyping，简称DM）核心是华盛顿 大学糖尿病研究中心（DRC）长期目标的核心是提高糖尿病- 华盛顿大学的相关研究，利用先进的服务和技术， 有助于发现可以转化为糖尿病和相关疾病的新疗法。的 CNOCore有三个目标：1）为DRC成员提供表型分析服务， 并提高NIH资助的糖尿病/代谢相关研究的成本效益; 2）提供 为DRC成员提供实验设计和表征方面的专家咨询和培训 与糖尿病、肥胖症和相关代谢疾病相关的小鼠模型;以及3）开发新的 研究能力，以提高DRC成员的能力，以执行糖尿病和代谢 research. The Core提供广泛的动物表型分析服务，包括a）身体组成 使用EchoMRI和/或DEXA扫描的分析; B）间接量热法以测量能量消耗; c）葡萄糖 和胰岛素耐受性测试; d）血压测定;和e）血清和胰岛素的生物化学分析。 组织来测量葡萄糖、甘油三酯、游离脂肪酸和胆固醇。满足糖尿病患者的关键需求 作为华盛顿大学的一个研究社区，核心为44个不同的DRC成员提供了服务 在过去的资助期内，有11个实验室获得了P&F资助。这些机构使用的服务 在这段时间内，研究人员对血清（> 4，800个样本）和组织（> 1，100个样本）进行了生化分析。 样品），>8500只小鼠的身体组成测定，以及> 2，400只小鼠的间接热量测定评估。 老鼠，更别提那些需求量最大的服务了。这些分析支持了130篇论文的发表。 核心不断发展，以响应用户的需要，通过消除一些服务和增加 他人新的和扩展的服务包括高胰岛素-正常血糖钳夹，产热表型， 扩大培训和咨询。共同努力，核心服务继续帮助刚果民主共和国调查人员 贡献重要的学术进步，具有巨大的转化潜力，以改善对患者的护理 患有糖尿病和相关并发症。