Core B: Animal Core and Germ-Free Mouse Core

核心B：动物核心和无菌小鼠核心

• 批准号: 10588796
• 负责人: Lucy Hoffmann Kennedy
• 金额: $ 11.44万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10588796
• 关键词: Accreditation; Adherence; American; Animal Experimentation; Animals; Behavior; Blood; Body Weight; Caring; Certification; Consultations; Derivation procedure; Diabetes Mellitus; Diet; Dose; Ensure; Environmental Monitoring; Experimental Designs; Fasting; Feces; Film; Genetic Engineering; Germ-Free; Glucose; Gnotobiotic; Goals; Guidelines; Health; Health Status; Housing; IACUC; Inbred BALB C Mice; Institution; Laboratories; Laboratory Animal Medicine; Laboratory mice; Maintenance; Michigan; Modeling; Monitor; Mus; Obesity; Personal Satisfaction; Phenotype; Play; Process; Protocols documentation; Quarantine; Reproducibility; Research; Research Personnel; Role; Services; Techniques; Technology; Training; United States Department of Agriculture; Universities; Urine; Veterinarians; Workplace; animal care; college; flexibility; germ free condition; metabolic phenotype; pathogen; sample collection; segregation; ventilation

Project Summary / Abstract – Animal Care and Germ-Free Mouse Core The Animal Care and Germ-Free Mouse Core of the Michigan MPMOD will play a central role in the management of animal flow through the Center’s MPB Phenotyping Core from initial acquisition to final disposition of imported mice. Both the Core Director and Associate Director are veterinarians and diplomates of the American College of Laboratory Animal Medicine. Specific functions of the Animal Care and Germ-Free Mouse Core include the coordination of mouse importation, quarantine, and environmental testing for a panel of murine pathogens, as well as providing housing, husbandry, and veterinary care of mice in accordance with the MPMOD (Administrative Core) guidelines. Research services such as hands-on technical support and germ-free technology and expertise will be unique components of our Core. Germ-free C57BL/6J, C57BL/6NCrl, BALB/c, and Swiss-Webster mice as well as several genetically engineered strains will be available to MPMOD investigators. In addition, investigators may import germ-free mice from other institutions or request rederivation of new germ-free strains. Experimental manipulations available for germ-free and SPF mice include routine treatments, sample collections, and in-house analysis (blood, feces, urine, etc.), training, and experimental design consultation, among others. Goals of the Animal Care and Germ-Free Mouse Core are to provide the highest level of health and well-being as well as veterinary expertise for all research animals in our USDA-certified and AAALAC-accredited facilities. All Core services will be performed under University of Michigan IACUC approved protocols. All SPF mice will be housed in Allentown ventilated racks. Germ-free or gnotobiotic mice will be maintained in flexible-film isolators or Tecniplast Isocage racks. Barrier conditions including cage changing in vertical laminar flow changing stations, strict adherence to microisolator techniques, and use of isolated rooms for colony segregation will assure maintenance of pathogen-free animals. The Animal Core will also provide oversight for technical support such as dose administration, obtaining body weights, fasting, glucose levels, providing and monitoring special diets, and transfer of mice to and from ULAM housing to the laboratories of the MPB Phenotyping Core.

项目摘要/摘要-爱护动物和无菌鼠芯 密歇根州MPMOD的动物保护和无菌小鼠核心将在 通过中心的MPB表型核心从最初获得到最终的动物流动管理 进口老鼠的处置。核心主任和副主任都是兽医和外交官 来自美国实验动物医学院。动物保育与无菌的特殊功能 鼠标核心包括协调鼠标输入、隔离和面板的环境测试 鼠类病原体，以及为小鼠提供住房、饲养和兽医护理 MPMOD(管理核心)指南。研究服务，如实践技术支持和 无菌技术和专业知识将是我们核心的独特组成部分。无菌C57BL/6J， C57BL/6NCrl，BALB/c，Swiss-Webster小鼠以及几个基因工程菌株将被 可供MPMOD调查人员使用。此外，调查人员可能会从其他机构进口无菌小鼠 或者要求重新衍生新的无菌菌株。可用于无菌和SPF的实验操作 小鼠包括常规治疗、样本采集和内部分析(血液、粪便、尿液等)、培训、 以及实验设计咨询等。爱护动物和无菌鼠芯的目标 为所有研究动物提供最高水平的健康和福祉以及兽医专业知识 在我们的USDA认证和AAALAC认证的设施中。所有核心服务将在加州大学 密歇根州IACUC批准的协议。所有SPF小鼠将被安置在Allentown通风架上。无菌或无菌 灵知生菌小鼠将被保存在软膜隔离器或Tecniplast等位架中。障碍条件 包括垂直层流换流站的换笼，严格遵守微隔离器技术， 使用隔离的房间进行群体隔离将确保维持无病原体的动物。这个 动物核心还将提供监督的技术支持，如剂量管理，获得身体 体重、禁食、血糖水平、提供和监测特殊饲料，以及来往乌兰姆的小鼠转移 住房到MPB表型核心的实验室。