The Role of Sleep in Innate Immune Homeostasis: Toward Mechanistic Understanding Through Genome-Wide Enhancer Analysis

睡眠在先天免疫稳态中的作用:通过全基因组增强子分析实现机制理解

基本信息

  • 批准号:
    10589548
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-10-01 至 2027-09-30
  • 项目状态:
    未结题

项目摘要

The sleep-immune axis is highly complex, and the negative impact of inadequate sleep on immune dysfunction is well recognized. Unfortunately, sleep disorders are highly prevalent among Veterans; as a result, understanding how sleep influences immune regulation offers novel therapeutic strategies for inflammatory diseases associated with poor sleep. The transcription factors (TFs) and gene regulatory programs involved in the sleep-immune axis are not fully understood. Analysis of genome-wide changes in the activity of cis-regulatory elements, or cistrome, offers an unbiased assessment of TF activity to identify key pathways regulating the sleep- immune axis. The studies I propose in this CDA-2 application will identify and test TF network pathways contributing to alterations in neutrophil function during sleep disruption. Because of my clinical practice in critical care medicine, I am focusing on understanding the mechanistic impact of sleep on sepsis. Using an established murine model of sleep fragmentation, I have identified phenotypes of immune dysfunction related to sleep disruption. Previous studies showed sleep disrupted mice had worse outcomes in pathogen-based sepsis models. Whether the mortality is a consequence of an early hyperinflammatory response or a sequala of poor pathogen clearance is uncertain. In this proposal, I specifically tested for the impact of sleep on the early inflammatory response to sepsis using the pathogen-free, endotoxin-induced peritonitis model. Mice with sleep disruption had less mortality than matched controls upon LPS-induced peritonitis, suggesting a dampened acute inflammatory response. Consistent with this notion, peritoneal neutrophils from sleep-disrupted septic mice have lower expression of Myeloperoxidase (Mpo), a critical enzyme in reactive oxygen species (ROS) production. Single-cell RNA sequencing from the bone marrow of sleep-disrupted mice showed global transcriptomic changes in mature neutrophils with activity signature of Stat3, a TF that mediates immunosuppressive response. These findings support my hypothesis that neutrophil phenotype and function are sensitive to sleep perturbation. Using the innovative approach of cistrome analysis, I aim to identify the mechanistic TF pathways by which sleep disruption impacts neutrophil function. The overarching goal is to identify pathways that could be targeted to modulate neutrophil activation to treat Veterans suffering from the adverse consequences of sleep disruption. This proposal addresses the impact of sleep on neutrophil function through transcriptional regulation. First, I will investigate whether sleep disruption reduces the fundamental capabilities of neutrophils, including ROS production, phagocytosis, and NETosis (AIM1). I will then use cistromic tools to test whether the sleep disruption affects the transcriptional activity of Stat3 (AIM2). Using ChIP-seq, I expect a global change in Stat3 binding in cis-regulatory elements at a genome-wide scale. I will corroborate the Stat3 analysis with an unbiased activity profiling of regulatory elements using a novel technique, capped-short RNA sequencing. Identifying where Stat3 binds and profiling the activity of these regulatory elements is a powerful strategy to determine Stat3 activity under sleep disruption. Finally, I will test the impact of sleep recovery on sepsis survival, neutrophil phenotype, and the kinetic changes in cistromic activity (AIM3). Understanding which TFs are activated during sleep recovery has the translational potential for restoring neutrophil function when sleep is persistently disrupted. The findings of these experiments will have a significant impact on the care of Veterans and advance the field by 1) identifying fundamental mechanisms of how healthy versus disrupted sleep affects neutrophils 2) refining the model of sleep-immune homeostasis during acute inflammatory responses; and 3) identifying new therapeutic targets and management strategies to modulate immune dysfunction due to sleep disruption, especially in situations where sleep is difficult to preserve or restore.
睡眠-免疫轴是高度复杂的,睡眠不足对免疫的负面影响

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Michael Tun Yin Lam其他文献

Dynamic activity in emcis/em-regulatory elements of leukocytes identifies transcription factor activation and stratifies COVID-19 severity in ICU patients
白细胞 emcis/em 调节元件中的动态活性可确定转录因子激活并对 ICU 患者的 COVID-19 严重程度进行分层
  • DOI:
    10.1016/j.xcrm.2023.100935
  • 发表时间:
    2023-02-21
  • 期刊:
  • 影响因子:
    10.600
  • 作者:
    Michael Tun Yin Lam;Sascha H. Duttke;Mazen F. Odish;Hiep D. Le;Emily A. Hansen;Celina T. Nguyen;Samantha Trescott;Roy Kim;Shaunak Deota;Max W. Chang;Arjun Patel;Mark Hepokoski;Mona Alotaibi;Mark Rolfsen;Katherine Perofsky;Anna S. Warden;Jennifer Foley;Sydney I. Ramirez;Jennifer M. Dan;Robert K. Abbott;Nicole G. Coufal
  • 通讯作者:
    Nicole G. Coufal

Michael Tun Yin Lam的其他文献

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