The Role of Sleep in Innate Immune Homeostasis: Toward Mechanistic Understanding Through Genome-Wide Enhancer Analysis

睡眠在先天免疫稳态中的作用：通过全基因组增强子分析实现机制理解

• 批准号: 10589548
• 负责人: Michael Tun Yin Lam
• 金额: --
• 依托单位: VA SAN DIEGO HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2027-09-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10589548
• 关键词: ATAC-seq; Activities of Daily Living; Acute; Address; Affect; Animals; Automobile Driving; Binding; Bone Marrow; Caring; Cells; ChIP-seq; Child Care; Chromatin; Chronic; Clinical; Complex; Coupled; Critical Care; Critical Illness; DNA Sequence; Data; Disease; Drowsiness; Endotoxins; Enhancers; Enzymes; Exhibits; Financial Hardship; Foundations; Gene Expression; Generations; Genetic Transcription; Genomics; Global Change; Goals; Health; Homeostasis; Immune; Immune System Diseases; Immune response; Immune system; Immunosuppression; Infection; Inflammation; Inflammatory; Inflammatory Response; Innate Immune System; Kinetics; Life; Link; Low Back Pain; Mediating; Medical; Medicine; Mental Depression; Mental Health; Modeling; Modernization; Molecular; Mus; Neutrophil Activation; Obstructive Sleep Apnea; Outcome; Pathway interactions; Patients; Peritoneal; Peritonitis; Peroxidases; Phagocytosis; Phenotype; Physicians; Population; Post-Traumatic Stress Disorders; Procedures; Production; Reactive Oxygen Species; Recovery; Regulator Genes; Regulatory Element; Reporting; Research; Risk; Role; Sepsis; Septic Shock; Shock; Signal Pathway; Signal Transduction; Sleep; Sleep Deprivation; Sleep Disorders; Sleep Fragmentations; Sleep disturbances; Sleeplessness; Societal Factors; Societies; Techniques; Testing; Time; Transcriptional Regulation; Translating; Vaccines; Veterans; adequate sleep; adverse outcome; chronic inflammatory disease; clinical practice; cytokine release syndrome; experience; experimental study; extracellular; genome-wide; genome-wide analysis; genomic tools; high risk; immune function; immunoregulation; improved; infection risk; innovation; insight; military veteran; mimetics; mortality; mouse model; neutrophil; new therapeutic target; novel; novel therapeutic intervention; pathogen; pharmacologic; poor sleep; preservation; programs; septic; shift work; single-cell RNA sequencing; sleep regulation; tool; transcription factor; transcriptome; transcriptome sequencing; transcriptomics; translational potential

The sleep-immune axis is highly complex, and the negative impact of inadequate sleep on immune dysfunction is well recognized. Unfortunately, sleep disorders are highly prevalent among Veterans; as a result, understanding how sleep influences immune regulation offers novel therapeutic strategies for inflammatory diseases associated with poor sleep. The transcription factors (TFs) and gene regulatory programs involved in the sleep-immune axis are not fully understood. Analysis of genome-wide changes in the activity of cis-regulatory elements, or cistrome, offers an unbiased assessment of TF activity to identify key pathways regulating the sleep- immune axis. The studies I propose in this CDA-2 application will identify and test TF network pathways contributing to alterations in neutrophil function during sleep disruption. Because of my clinical practice in critical care medicine, I am focusing on understanding the mechanistic impact of sleep on sepsis. Using an established murine model of sleep fragmentation, I have identified phenotypes of immune dysfunction related to sleep disruption. Previous studies showed sleep disrupted mice had worse outcomes in pathogen-based sepsis models. Whether the mortality is a consequence of an early hyperinflammatory response or a sequala of poor pathogen clearance is uncertain. In this proposal, I specifically tested for the impact of sleep on the early inflammatory response to sepsis using the pathogen-free, endotoxin-induced peritonitis model. Mice with sleep disruption had less mortality than matched controls upon LPS-induced peritonitis, suggesting a dampened acute inflammatory response. Consistent with this notion, peritoneal neutrophils from sleep-disrupted septic mice have lower expression of Myeloperoxidase (Mpo), a critical enzyme in reactive oxygen species (ROS) production. Single-cell RNA sequencing from the bone marrow of sleep-disrupted mice showed global transcriptomic changes in mature neutrophils with activity signature of Stat3, a TF that mediates immunosuppressive response. These findings support my hypothesis that neutrophil phenotype and function are sensitive to sleep perturbation. Using the innovative approach of cistrome analysis, I aim to identify the mechanistic TF pathways by which sleep disruption impacts neutrophil function. The overarching goal is to identify pathways that could be targeted to modulate neutrophil activation to treat Veterans suffering from the adverse consequences of sleep disruption. This proposal addresses the impact of sleep on neutrophil function through transcriptional regulation. First, I will investigate whether sleep disruption reduces the fundamental capabilities of neutrophils, including ROS production, phagocytosis, and NETosis (AIM1). I will then use cistromic tools to test whether the sleep disruption affects the transcriptional activity of Stat3 (AIM2). Using ChIP-seq, I expect a global change in Stat3 binding in cis-regulatory elements at a genome-wide scale. I will corroborate the Stat3 analysis with an unbiased activity profiling of regulatory elements using a novel technique, capped-short RNA sequencing. Identifying where Stat3 binds and profiling the activity of these regulatory elements is a powerful strategy to determine Stat3 activity under sleep disruption. Finally, I will test the impact of sleep recovery on sepsis survival, neutrophil phenotype, and the kinetic changes in cistromic activity (AIM3). Understanding which TFs are activated during sleep recovery has the translational potential for restoring neutrophil function when sleep is persistently disrupted. The findings of these experiments will have a significant impact on the care of Veterans and advance the field by 1) identifying fundamental mechanisms of how healthy versus disrupted sleep affects neutrophils 2) refining the model of sleep-immune homeostasis during acute inflammatory responses; and 3) identifying new therapeutic targets and management strategies to modulate immune dysfunction due to sleep disruption, especially in situations where sleep is difficult to preserve or restore.

睡眠-免疫轴是高度复杂的，睡眠不足对免疫的负面影响