Advanced Tissue Pathology and Imaging

摘要 可视化和量化组织和细胞病理是理解发病机制的核心， 代谢疾病的进展和并发症，并监测其治疗的有效性。 标记、成像和量化细胞成分的方法已经迅速改进，并且正在提供新的 在亚细胞、细胞和整个器官水平评估表型的能力。先进的组织 病理学和成像核心（ATPI）在当前的资助周期中需求很大， 自上次提交报告以来，为满足刚果民主共和国调查人员不断变化的需求，该报告的编写工作有了很大变化。 ATPI已被证明在实现其协助个人调查员的使命方面取得了广泛的成功， 组织表型最初设想和组织作为一个组织学的核心，随后扩大到 包括显微镜，ATPI不断发展，以满足DRC调查人员不断变化的需求。这是 在当前的资助期内成功地进行了重组和扩展，以包括最先进的清算， 分子“染色”和成像技术。为了满足对这些服务的大量和不断增长的需求， 在上一个供资周期，我们简化了在调查员调查中确定并由审查人员推荐的 通过建立在线服务请求系统和加强努力， 为最重的用户提供所需的设备和培训，以执行许多 技术为自己。一位在大脑和大脑中实施这些先进技术的专家 洛里·泽尔泽博士领导了核心的重组。ATPI服务补充 其他DRC核心提供的信息，以便利用DRC资源的调查人员能够充分利用 表征小鼠的组织学、免疫学和代谢功能和表型。因此， 哥伦比亚、Naomi Berrie糖尿病中心和DRC的投资已经被用来提供 优质的服务，低成本。

ABSTRACT Visualizing and quantifying tissue and cellular pathologies are central to understanding the pathogenesis, progression, and complications of metabolic disease, and to monitor the effectiveness of its treatment. Methods of labeling, imaging, and quantifying cellular elements have improved rapidly and are providing new capabilities to evaluate phenotypes at the sub-cellular, cellular, and whole-organ levels. The Advanced Tissue Pathology and Imaging Core (ATPI) has been in great demand during the current funding cycle and has evolved significantly since the last submission to meet the changing needs of DRC investigators. The ATPI has proven broadly successful in achieving its mission of assisting individual investigators in histophenotyping. Originally conceived and organized as a histology core and subsequently expanded to include microscopy, the ATPI continues to evolve to meet the everchanging needs of DRC investigators. It was successfully reorganized and expanded during the current funding period to include state-of-the-art clearing, molecular “staining” and imaging technologies. To meet the large and growing demands for these services identified in investigator surveys and recommended by reviewers in the previous funding cycle, we streamlined the administrative components of the ATPI by creating an on-line service request system and increased efforts to provide the heaviest users with the access to equipment and training needed to perform many of the techniques for themselves. An expert in the implementation of these advanced techniques in both the brain and peripheral organs, Dr. Lori Zeltser has led the restructuring of the Core. The ATPI services complement those provided by other DRC Cores, so that investigators who take advantage of DRC resources can fully characterize the histologic, immunologic, and metabolic function and phenotypes of mice. Thus, the combined investments by Columbia, the Naomi Berrie Diabetes Center and the DRC have been leveraged to provide excellent services at low costs.