Studies of a new checkpoint regulator in the control of intestinal inflammation

控制肠道炎症的新检查点调节剂的研究

• 批准号: 10271880
• 负责人: Zhiqiang Zhang
• 金额: $ 40.38万
• 依托单位: METHODIST HOSPITAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-07 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10271880
• 关键词: Address; Antifungal Agents; Autoimmunity; Biological Assay; Biological Response Modifiers; Candida albicans; Caspase; Cell physiology; Cells; Chronic; Colitis; DNA Methylation; DNA mapping; Dendritic Cells; Disease; Environment; Epigenetic Process; Epithelial; Epithelial Cells; Family; Family member; Gastrointestinal tract structure; Gene Expression; Genes; Homeostasis; Host Defense; Human; Hyperactivity; Immune; Immune System Diseases; Immune response; Immunity; Infection; Inflammasome; Inflammation; Inflammatory; Inflammatory Response; Innate Immune Response; Innate Immune System; Interleukin-1; Interleukin-1 beta; Interleukin-18; Intestines; Invaded; Investigation; Knockout Mice; Lead; Link; Lupus; Mediating; Modeling; Modification; Molecular; Mucosal Immune System; Mus; Mycoses; Natural Immunity; Pathogenicity; Pathway interactions; Physiological; Play; Positioning Attribute; Production; Proteins; RNA Virus Infections; Receptor Signaling; Respiratory System; Role; Scaffolding Protein; Signal Transduction; Site; Stimulus; System; T-Lymphocyte; TRIM Family; Testing; Therapeutic; Tissues; Toll-like receptors; Ubiquitination; Work; cell type; chromatin modification; cytokine; fungus; gain of function; gastrointestinal; histone modification; immune activation; in vivo; inflammatory disease of the intestine; innate immune function; innate immune mechanisms; insight; intestinal epithelium; loss of function; macrophage; microorganism; mouse model; multicatalytic endopeptidase complex; novel; novel strategies; pathogen; protein degradation; response; scaffold; screening; tool; treatment strategy; ubiquitin-protein ligase

Project Summary The innate immune system is the first line of host defense against invading pathogenic microorganisms. Innate immune cells can recognize these pathogens to induce cytokines against infections. However, this immune response must be tightly regulated to effectively eliminate invading microorganisms while minimizing tissue inflammation. Protein ubiquitination is important both in signal transduction and in proteasome-mediated protein degradation and is a pivotal regulatory mechanism for the innate immune system. A key component of the ubiquitination system is E3 ligase to specifically recognize the substrate for modification. We recently found that a TRIM family member, E3 ligase TRIM29, is highly expressed in intestinal epithelial cells (IECs). IECs act as a physical barrier between the external environment and the mucosal immune system. Importantly, after activation, IECs become innate immune-like cells to produce robust proinflammatory cytokines. We posit that TRIM29 is a key immune regulator to suppress innate immune response in a cell/tissue-specific manner to reduce intestinal inflammation. Notably, in the gut, genes encoding inflammasome components such as CARD9, NLRP3 and Caspases are associated with intestinal inflammation, and mice deficient in these genes are susceptible to colitis and fungal infection. However, hyperactive inflammasome signaling and uncontrolled IL-1 family cytokines lead to immune disorders and inflammatory disease. For instance, overproduction of inflammasome cytokine IL-18, excessive activation of NLRP3-inflammasome, or aberrant immune response to fungus play a major pathogenic role in colitis. Thus, this immune response must be finely tuned during immune activation for optimal protection against infections while avoiding unwanted inflammation. However, little is known about how such activation is terminated. Our preliminary studies show that TRIM29 negatively regulates IL-1 family cytokines produced by IECs during colitis or fungal infection. Moreover, expression of TRIM29 itself is significantly downregulated in inflamed colons, whereas production of IL-1 family cytokines is elevated. However, it is unknown whether lost expression of TRIM29 is regulated by IL-1 family cytokines and/or epigenetic modifications to control responses to environment stimuli in the intestines. We hypothesize that TRIM29 expression in IECs is a checkpoint regulator of IL-1 family cytokines crucial to reduce intestinal inflammation in colitis and fungal infection. We propose the following three aims to test this hypothesis: (1) To dissect molecular pathways that control TRIM29 expression in epithelial cells under normal and inflammatory conditions; (2) To define the mechanism by which TRIM29 inhibits IL-1 inflammation; (3) To investigate in vivo functions of TRIM29 in regulating IECs function in colitis and anti-fungal immunity.

项目总结