Antimicrobial Peptides as Probes for Electrochemical Detection of Methicillin-resistant Staphylococcus Aureus

抗菌肽作为电化学检测耐甲氧西林金黄色葡萄球菌的探针

• 批准号: 10271841
• 负责人: Rebecca Y Lai
• 金额: $ 11.15万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10271841
• 关键词: Address; Admission activity; Affinity; Antibodies; Area; Attention; Biological; Biological Assay; Biosensing Techniques; Buffers; Carbon; Cells; Chemistry; Collaborations; Communication; Communication Research; Consumption; Detection; Development; Devices; Diagnosis; Disease; Early identification; Electrochemistry; Electrodes; Elements; Ensure; Future; Generations; Genus staphylococcus; Goals; Gold; Health care facility; Healthcare Systems; Hospitals; Immunoassay; Infection; Ions; Knowledge; Length; Length of Stay; Life; Medical center; Metals; Methods; Microfluidic Microchips; Microfluidics; Modification; Morbidity - disease rate; Multi-Drug Resistance; Nebraska; Nosocomial Infections; Outcome; Paper; Patients; Peptide Fragments; Peptides; Polymerase Chain Reaction; Property; Proteins; Reaction; Reaction Time; Reagent; Research; Resources; Saliva; Sampling; Spectrum Analysis; Staphylococcus aureus; Staphylococcus aureus infection; Surface Plasmon Resonance; Technology; Testing; Time; Universities; antimicrobial peptide; base; cost; cost effective; design; detection limit; detection method; disease diagnosis; healthcare-associated infections; innovation; methicillin resistant Staphylococcus aureus; mortality; nasal swab; novel; resistant strain; sensor

PROJECT SUMMARY/ABSTRACT The broad, long-term goal of Project Leader Rebecca Lai’s project is to develop a near real-time method for detecting methicillin-resistant Staphylococcus aureus (MRSA). In recent decades, MRSA strains have become the most common cause of health care-associated infections, and new multi-drug-resistant strains of this severe and potentially life-threatening disease have emerged. Although implementation of universal admission surveillance can reduce the spread of MRSA infections, it is not practiced in many health care systems because of the cost of testing. Thus, there is a critical need for more advanced detection methods that are sensitive, specific, and rapid yet cost-effective enough to be employed in hospitals and health care facilities in low resource settings. An area that deserves attention in MRSA sensor design is the selection of appropriate biorecognition elements. Among available biorecognition elements, antimicrobial peptides (AMPs), which are short peptide fragments existing in various forms of life, are one of the best alternatives to antibodies because of a combination of attractive properties: high stability, synthetic simplicity, easy accessibility, and high affinity toward their specific bacterial targets. Thus, the goal of this project is to evaluate and characterize MRSA-specific peptides suitable for use as biorecognition elements in the fabrication of E-PB MRSA sensors and to use potential-assisted “click” chemistry in the construction of a paper-based sensor array. Two aims will be achieved: 1) design and fabricate E-PB sensors using high efficacy AMPs for detection of MRSA and 2) develop a paper-based E-PB sensor array using potential-assisted “click” chemistry. To ensure that the sensor array will perform optimally, critical aspects of the bioconjugation reaction will be systematically evaluated, including the reaction time, applied potential, and concentrations of the reagents. This project will result in an E-PB sensor array capable of near real-time detection of MRSA bacterial cells, which will be an important step toward the development of an all-in-one microfluidic- based MRSA sensor device. Project outcomes will stimulate future research on AMPs for biosensing applications. The availability of such a versatile sensing platform could have a major impact on MRSA diagnosis, management, and surveillance. This project will also contribute to the wider goal of the Nebraska Center for Integrated Biomolecular Communication (CIBC), which includes the development of novel biomolecular sensing strategies for disease diagnosis. It will enhance CIBC’s research strengths in biosensing and bioanalysis and facilitate future development of sensors for other important diseases.

项目摘要/摘要 项目负责人Rebecca Lai的项目的广泛、长期的目标是开发一种近乎实时的方法 检测耐甲氧西林金黄色葡萄球菌(MRSA)。近几十年来，MRSA菌株已经成为 卫生保健相关感染的最常见原因，以及这种严重的新的多重耐药菌株 潜在威胁生命的疾病已经出现。虽然实施了全民入学 监测可以减少MRSA感染的传播，但在许多医疗保健系统中没有实施，因为 测试的成本。因此，迫切需要更先进的、灵敏的检测方法， 具体、快速但成本效益高，足以在资源较少的医院和卫生保健机构中使用 设置。在MRSA传感器设计中值得注意的一个方面是选择合适的生物识别 元素。在现有的生物识别元件中，抗菌肽是一种短肽 存在于各种形式的生命中的片段，是抗体的最佳替代品之一，因为它们的结合 极具吸引力的特性：高稳定性、合成简单、易于获得以及对其特定特性的高亲和力 细菌靶标。因此，本项目的目标是评估和鉴定适合mrsa的特异性多肽。 用作E-PB MRSA传感器制造中的生物识别元件，并使用电势辅助的“点击” 化学在构建纸基传感器阵列中的作用。将实现两个目标：1)设计和制造 利用高效AMPS检测MRSA的E-PB传感器和2)研制了纸基E-PB传感器阵列 利用电势辅助的“点击”化学。为了确保传感器阵列将以最佳状态运行，关键方面 将对生物偶联反应的性能进行系统的评估，包括反应时间、施加电位和 试剂的浓度。该项目将产生能够近乎实时检测的E-PB传感器阵列 这将是朝着发展一体化微流控技术迈出的重要一步。 基于MRSA的传感器设备。项目成果将刺激未来用于生物传感的AMP研究 申请。这种多功能传感平台的可用性可能会对MRSA诊断产生重大影响， 管理和监控。该项目还将有助于内布拉斯加州中心实现更广泛的目标 集成生物分子通信(CIBC)，包括新型生物分子传感的发展 疾病诊断策略。它将增强CIBC在生物传感和生物分析方面的研究实力 促进其他重要疾病传感器的未来发展。