Antimicrobial Peptides as Probes for Electrochemical Detection of Methicillin-resistant Staphylococcus Aureus

项目总结/摘要 项目负责人Rebecca Lai的项目的长期目标是开发一种近实时方法， 检测耐甲氧西林金黄色葡萄球菌（MRSA）。近几十年来，MRSA菌株已成为 最常见的原因，卫生保健相关的感染，和新的多重耐药菌株，这种严重的 可能危及生命的疾病已经出现。虽然实施普遍入学 监测可以减少MRSA感染的传播，但在许多卫生保健系统中没有实施， 测试的成本。因此，迫切需要更先进的检测方法， 具体、快速且具有成本效益，足以在资源较少医院和卫生保健设施中使用 设置. MRSA传感器设计中值得关注的一个领域是选择适当的生物识别 元素在现有的生物识别元件中，抗菌肽（AMP）是短肽， 存在于各种形式的生命中的片段，是抗体的最佳替代品之一，因为它们结合在一起， 具有吸引人的特性：高稳定性，合成简单，易于获得，以及对它们的特异性高亲和力。 细菌目标。因此，本项目的目标是评估和表征MRSA特异性肽， 用于在E-PB MRSA传感器的制造中用作生物识别元件， 化学在纸基传感器阵列的构造中。主要实现两个目标：1）设计和制造 使用高效AMP检测MRSA的E-PB传感器和2）开发基于纸的E-PB传感器阵列 使用电势辅助的“点击”化学。为了确保传感器阵列的最佳性能， 将系统地评估生物缀合反应的条件，包括反应时间、施加的电位和 试剂的浓度。该项目将导致一个E-PB传感器阵列能够近实时检测 MRSA细菌细胞，这将是朝着开发一体化微流体的重要一步- 基于MRSA的传感器设备。项目成果将刺激未来对生物传感AMP的研究 应用.这种多功能传感平台的可用性可能对MRSA诊断产生重大影响， 管理和监督。该项目还将有助于实现内布拉斯加州中心的更广泛目标， 集成生物分子通信（CIBC），包括开发新型生物分子传感 疾病诊断的策略。它将增强CIBC在生物传感和生物分析方面的研究实力， 促进其他重要疾病传感器的未来发展。

PROJECT SUMMARY/ABSTRACT The broad, long-term goal of Project Leader Rebecca Lai’s project is to develop a near real-time method for detecting methicillin-resistant Staphylococcus aureus (MRSA). In recent decades, MRSA strains have become the most common cause of health care-associated infections, and new multi-drug-resistant strains of this severe and potentially life-threatening disease have emerged. Although implementation of universal admission surveillance can reduce the spread of MRSA infections, it is not practiced in many health care systems because of the cost of testing. Thus, there is a critical need for more advanced detection methods that are sensitive, specific, and rapid yet cost-effective enough to be employed in hospitals and health care facilities in low resource settings. An area that deserves attention in MRSA sensor design is the selection of appropriate biorecognition elements. Among available biorecognition elements, antimicrobial peptides (AMPs), which are short peptide fragments existing in various forms of life, are one of the best alternatives to antibodies because of a combination of attractive properties: high stability, synthetic simplicity, easy accessibility, and high affinity toward their specific bacterial targets. Thus, the goal of this project is to evaluate and characterize MRSA-specific peptides suitable for use as biorecognition elements in the fabrication of E-PB MRSA sensors and to use potential-assisted “click” chemistry in the construction of a paper-based sensor array. Two aims will be achieved: 1) design and fabricate E-PB sensors using high efficacy AMPs for detection of MRSA and 2) develop a paper-based E-PB sensor array using potential-assisted “click” chemistry. To ensure that the sensor array will perform optimally, critical aspects of the bioconjugation reaction will be systematically evaluated, including the reaction time, applied potential, and concentrations of the reagents. This project will result in an E-PB sensor array capable of near real-time detection of MRSA bacterial cells, which will be an important step toward the development of an all-in-one microfluidic- based MRSA sensor device. Project outcomes will stimulate future research on AMPs for biosensing applications. The availability of such a versatile sensing platform could have a major impact on MRSA diagnosis, management, and surveillance. This project will also contribute to the wider goal of the Nebraska Center for Integrated Biomolecular Communication (CIBC), which includes the development of novel biomolecular sensing strategies for disease diagnosis. It will enhance CIBC’s research strengths in biosensing and bioanalysis and facilitate future development of sensors for other important diseases.