Mitochondrial iron export therapy for doxorubicin-induced cardiotoxicity

线粒体铁输出疗法治疗阿霉素诱导的心脏毒性

基本信息

  • 批准号:
    10561788
  • 负责人:
  • 金额:
    $ 66.63万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-01-01 至 2026-12-31
  • 项目状态:
    未结题

项目摘要

PROJECT SUMMARY/ABSTRACT Doxorubicin is routinely prescribed in treatment of various cancers because of its extremely high efficacy. However, its use is severely limited because of its potential to cause irreversible cardiotoxicity. Since cessation of therapy is not viable in cancer patients, there is a need to explore the molecular mechanisms underlying cardiotoxicity to accurately identify risk factors as well as therapeutic targets for effective adjuncts. The primary mechanism by which doxorubicin exerts its cardiotoxic effects is due to preferential accumulation of excess iron in cardiac mitochondria, which generates cytotoxic free radicals, and disruption of cellular and subcellular iron utilization. Thus, chelating excess mitochondrial iron can prevent doxorubicin-induced cardiac dysfunction. Indeed, the only drug approved to treat doxorubicin cardiomyopathy, dexrazoxane, has demonstrated mitochondrial chelation potential. However, dexrazoxane alters topoisomerases, the enzymes responsible for DNA replication and doxorubicin’s pharmacological target, which thereby impairs doxorubicin’s anticancer activity. In addition, dexrazoxane has potential to induce fatal myelosuppression and acute leukemias, which consequently limit its clinical utility. Cancer survivors who subsequently develop cardiomyopathies have the worst survivals among all cardiomyopathies, and timely intervention results in a superior clinical outcome in those survivors treated with cardiotoxic chemotherapy. Thus, there is a major unmet need for mitochondria-specific iron chelators that do not impede doxorubicin’s antitumor activity. Earlier we have demonstrated that hinokitiol, a small molecule with high iron binding affinity and cell permeability, corrects abnormal iron buildup across the membrane caused by genetic deficiency in mitochondrial iron transporters. These findings prompted us to question if hinokitiol could rescue doxorubicin-induced mitochondrial accumulation of iron. Our pilot study has indicated a feasibility that hinokitiol corrects mitochondrial iron overload and improves survival in cardiac cells treated with doxorubicin with no influence on tumor-killing effect of doxorubicin. Thus, we hypothesize that hinokitiol mobilizes excess iron from the cardiac mitochondria and prevents oxidative damage, thereby reversing doxorubicin-induced cardiomyopathy, while preserving doxorubicin’s anticancer activity. The specific aims are to determine: i) mitochondrial iron export after hinokitiol administration, ii) the cardioprotective effect of hinokitiol on doxorubicin-induced cardiotoxicity, and iii) the effect of hinokitiol on the antineoplastic efficacy of doxorubicin using tumor-bearing mice. Our studies will provide a new therapeutic strategy to reverse abnormal accumulation of mitochondrial iron and correct doxorubicin-induced cardiotoxicity without compromising its antineoplastic effects. If successful, this drug can be safely co-administered with doxorubicin as a rescue factor to improve the therapeutic index of doxorubicin along with better clinical outcome. .
项目概要/摘要 阿霉素因其极高的功效而被常规用于治疗各种癌症。 然而,由于其可能导致不可逆的心脏毒性,其使用受到严重限制。自从停止以来 这种治疗方法对于癌症患者来说是不可行的,因此需要探索其潜在的分子机制 心脏毒性,以准确识别危险因素以及有效辅助治疗的目标。初级 阿霉素发挥心脏毒性作用的机制是由于过量铁的优先积累 在心脏线粒体中,产生细胞毒性自由基,并破坏细胞和亚细胞铁 利用率。因此,螯合过量的线粒体铁可以预防阿霉素引起的心脏功能障碍。 事实上,唯一被批准用于治疗阿霉素心肌病的药物右雷佐生已被证明 线粒体螯合电位。然而,右雷佐生会改变拓扑异构酶,这种酶负责 DNA 复制和阿霉素的药理靶点,从而损害阿霉素的抗癌作用 活动。此外,右雷佐生有可能诱发致命的骨髓抑制和急性白血病, 从而限制了其临床应用。随后患上心肌病的癌症幸存者有 所有心肌病中生存率最差的患者,及时干预可带来优异的临床结果 接受心脏毒性化疗的幸存者。因此,线粒体特异性的需求尚未得到满足 不妨碍阿霉素抗肿瘤活性的铁螯合剂。早些时候我们已经证明了扁柏酚, 具有高铁结合亲和力和细胞渗透性的小分子,可纠正整个细胞内异常的铁积聚 由线粒体铁转运蛋白遗传缺陷引起的膜。这些发现促使我们 质疑扁柏酚是否可以挽救阿霉素诱导的线粒体铁积累。我们的试点研究有 表明扁柏酚可以纠正线粒体铁超载并提高心肌细胞的存活率 用阿霉素治疗,不影响阿霉素的杀瘤效果。因此,我们假设 扁柏酚可动员心脏线粒体中多余的铁,防止氧化损伤,从而逆转 阿霉素诱导的心肌病,同时保留阿霉素的抗癌活性。具体目标是 确定:i) 桧木醇给药后线粒体铁输出,ii) 桧木酚的心脏保护作用 对阿霉素诱导的心脏毒性的影响,以及 iii) 扁柏酚对阿霉素抗肿瘤功效的影响 使用荷瘤小鼠。我们的研究将为逆转异常积累提供新的治疗策略 线粒体铁并纠正阿霉素引起的心脏毒性而不损害其抗肿瘤作用 影响。如果成功,该药物可以安全地与多柔比星联合给药,作为救援因素,以改善病情 阿霉素的治疗指数以及更好的临床结果。 。

项目成果

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Jonghan Kim其他文献

Jonghan Kim的其他文献

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{{ truncateString('Jonghan Kim', 18)}}的其他基金

Restoration of mitochondrial function by small-molecule iron transporter in Friedreich’s ataxia
小分子铁转运蛋白在弗里德赖希共济失调中恢复线粒体功能
  • 批准号:
    10451180
  • 财政年份:
    2022
  • 资助金额:
    $ 66.63万
  • 项目类别:
Restoration of Mitochondrial Function by Small-Molecule Iron Transporter in Friedreich’s Ataxia
小分子铁转运蛋白对弗里德赖希共济失调线粒体功能的恢复
  • 批准号:
    10558616
  • 财政年份:
    2022
  • 资助金额:
    $ 66.63万
  • 项目类别:
Influence of HFE on metal pharmacokinetics and neurotoxicity
HFE 对金属药代动力学和神经毒性的影响
  • 批准号:
    8536288
  • 财政年份:
    2012
  • 资助金额:
    $ 66.63万
  • 项目类别:
Influence of HFE on metal pharmacokinetics and neurotoxicity
HFE 对金属药代动力学和神经毒性的影响
  • 批准号:
    8719103
  • 财政年份:
    2012
  • 资助金额:
    $ 66.63万
  • 项目类别:
Influence of HFE on metal pharmacokinetics and neurotoxicity
HFE 对金属药代动力学和神经毒性的影响
  • 批准号:
    8525694
  • 财政年份:
    2012
  • 资助金额:
    $ 66.63万
  • 项目类别:
Influence of HFE on metal pharmacokinetics and neurotoxicity
HFE 对金属药代动力学和神经毒性的影响
  • 批准号:
    8019097
  • 财政年份:
    2010
  • 资助金额:
    $ 66.63万
  • 项目类别:
Influence of HFE on metal pharmacokinetics and neurotoxicity
HFE 对金属药代动力学和神经毒性的影响
  • 批准号:
    7771472
  • 财政年份:
    2010
  • 资助金额:
    $ 66.63万
  • 项目类别:

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